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Production of Graft Copolymers of Cod Collagen with Butyl Acrylate and Vinyl Butyl Ether in the Presence of Triethylborane—Prospects for Use in Regenerative Medicine

Collagen is a suitable material for regenerative medicine because it is characterized by its good biocompatibility. However, due to its fibrillar structure, it cannot organize itself into three-dimensional porous structures without additional modification. The introduction of synthetic monomer eleme...

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Autores principales: Semenycheva, Lyudmila, Chasova, Victoria O., Pegeev, Nikita L., Uromicheva, Marina A., Mitin, Alexander V., Kuznetsova, Yulia L., Farafontova, Ekaterina A., Rubtsova, Yulia P., Linkova, Daria D., Egorikhina, Marfa N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421105/
https://www.ncbi.nlm.nih.gov/pubmed/37571053
http://dx.doi.org/10.3390/polym15153159
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author Semenycheva, Lyudmila
Chasova, Victoria O.
Pegeev, Nikita L.
Uromicheva, Marina A.
Mitin, Alexander V.
Kuznetsova, Yulia L.
Farafontova, Ekaterina A.
Rubtsova, Yulia P.
Linkova, Daria D.
Egorikhina, Marfa N.
author_facet Semenycheva, Lyudmila
Chasova, Victoria O.
Pegeev, Nikita L.
Uromicheva, Marina A.
Mitin, Alexander V.
Kuznetsova, Yulia L.
Farafontova, Ekaterina A.
Rubtsova, Yulia P.
Linkova, Daria D.
Egorikhina, Marfa N.
author_sort Semenycheva, Lyudmila
collection PubMed
description Collagen is a suitable material for regenerative medicine because it is characterized by its good biocompatibility. However, due to its fibrillar structure, it cannot organize itself into three-dimensional porous structures without additional modification. The introduction of synthetic monomer elements into the collagen macromolecules is a technique used to form three-dimensional, collagen-based, branched, and crosslinked structures. New types of graft copolymers made from cod collagen with a butyl acrylate and vinyl butyl ether copolymer in aqueous dispersion were obtained in the presence of triethylborane by a radical mechanism. The process of graft copolymer formation proceeded as usual by radical initiation, through radicals formed during triethylborane oxidation by oxygen residues, collagen borination, and reversible inhibition with the participation of a boroxyl radical. The characteristics of the graft copolymers were determined using methods of physical and chemical analysis (GPC, SEM, IR spectroscopy, etc.), while the cytotoxicity was assessed using the MTT assay method. It is shown that the grafting of alternating blocks of butyl acrylate and vinyl butyl ether to the protein macromolecules results in changes in the morphological pattern of the graft co-polymer in comparison with native collagen. This is manifested in the development of consolidations around the collagen fibers of the structural matrices, with the co-polymer cellular structure consisting of interpenetrating pores of unequal size. Additionally, it is important that the graft co-polymer solutions are not toxic at a certain concentration. The above properties confirm the promising nature of the technique’s application as the basis for producing new materials for regenerative medicine.
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spelling pubmed-104211052023-08-12 Production of Graft Copolymers of Cod Collagen with Butyl Acrylate and Vinyl Butyl Ether in the Presence of Triethylborane—Prospects for Use in Regenerative Medicine Semenycheva, Lyudmila Chasova, Victoria O. Pegeev, Nikita L. Uromicheva, Marina A. Mitin, Alexander V. Kuznetsova, Yulia L. Farafontova, Ekaterina A. Rubtsova, Yulia P. Linkova, Daria D. Egorikhina, Marfa N. Polymers (Basel) Article Collagen is a suitable material for regenerative medicine because it is characterized by its good biocompatibility. However, due to its fibrillar structure, it cannot organize itself into three-dimensional porous structures without additional modification. The introduction of synthetic monomer elements into the collagen macromolecules is a technique used to form three-dimensional, collagen-based, branched, and crosslinked structures. New types of graft copolymers made from cod collagen with a butyl acrylate and vinyl butyl ether copolymer in aqueous dispersion were obtained in the presence of triethylborane by a radical mechanism. The process of graft copolymer formation proceeded as usual by radical initiation, through radicals formed during triethylborane oxidation by oxygen residues, collagen borination, and reversible inhibition with the participation of a boroxyl radical. The characteristics of the graft copolymers were determined using methods of physical and chemical analysis (GPC, SEM, IR spectroscopy, etc.), while the cytotoxicity was assessed using the MTT assay method. It is shown that the grafting of alternating blocks of butyl acrylate and vinyl butyl ether to the protein macromolecules results in changes in the morphological pattern of the graft co-polymer in comparison with native collagen. This is manifested in the development of consolidations around the collagen fibers of the structural matrices, with the co-polymer cellular structure consisting of interpenetrating pores of unequal size. Additionally, it is important that the graft co-polymer solutions are not toxic at a certain concentration. The above properties confirm the promising nature of the technique’s application as the basis for producing new materials for regenerative medicine. MDPI 2023-07-25 /pmc/articles/PMC10421105/ /pubmed/37571053 http://dx.doi.org/10.3390/polym15153159 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Semenycheva, Lyudmila
Chasova, Victoria O.
Pegeev, Nikita L.
Uromicheva, Marina A.
Mitin, Alexander V.
Kuznetsova, Yulia L.
Farafontova, Ekaterina A.
Rubtsova, Yulia P.
Linkova, Daria D.
Egorikhina, Marfa N.
Production of Graft Copolymers of Cod Collagen with Butyl Acrylate and Vinyl Butyl Ether in the Presence of Triethylborane—Prospects for Use in Regenerative Medicine
title Production of Graft Copolymers of Cod Collagen with Butyl Acrylate and Vinyl Butyl Ether in the Presence of Triethylborane—Prospects for Use in Regenerative Medicine
title_full Production of Graft Copolymers of Cod Collagen with Butyl Acrylate and Vinyl Butyl Ether in the Presence of Triethylborane—Prospects for Use in Regenerative Medicine
title_fullStr Production of Graft Copolymers of Cod Collagen with Butyl Acrylate and Vinyl Butyl Ether in the Presence of Triethylborane—Prospects for Use in Regenerative Medicine
title_full_unstemmed Production of Graft Copolymers of Cod Collagen with Butyl Acrylate and Vinyl Butyl Ether in the Presence of Triethylborane—Prospects for Use in Regenerative Medicine
title_short Production of Graft Copolymers of Cod Collagen with Butyl Acrylate and Vinyl Butyl Ether in the Presence of Triethylborane—Prospects for Use in Regenerative Medicine
title_sort production of graft copolymers of cod collagen with butyl acrylate and vinyl butyl ether in the presence of triethylborane—prospects for use in regenerative medicine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421105/
https://www.ncbi.nlm.nih.gov/pubmed/37571053
http://dx.doi.org/10.3390/polym15153159
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