The Small RNA Component of Arabidopsis thaliana Phloem Sap and Its Response to Iron Deficiency

In order to discover sRNA that might function during iron deficiency stress, RNA was prepared from phloem exudates of Arabidopsis thaliana, and used for RNA-seq. Bioanalyzer results indicate that abundant RNA from phloem is small in size—less than 200 nt. Moreover, typical rRNA bands were not observ...

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Autores principales: Bakirbas, Ahmet, Castro-Rodriguez, Rosario, Walker, Elsbeth L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421156/
https://www.ncbi.nlm.nih.gov/pubmed/37570935
http://dx.doi.org/10.3390/plants12152782
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author Bakirbas, Ahmet
Castro-Rodriguez, Rosario
Walker, Elsbeth L.
author_facet Bakirbas, Ahmet
Castro-Rodriguez, Rosario
Walker, Elsbeth L.
author_sort Bakirbas, Ahmet
collection PubMed
description In order to discover sRNA that might function during iron deficiency stress, RNA was prepared from phloem exudates of Arabidopsis thaliana, and used for RNA-seq. Bioanalyzer results indicate that abundant RNA from phloem is small in size—less than 200 nt. Moreover, typical rRNA bands were not observed. Sequencing of eight independent phloem RNA samples indicated that tRNA-derived fragments, specifically 5′ tRFs and 5′ tRNA halves, are highly abundant in phloem sap, comprising about 46% of all reads. In addition, a set of miRNAs that are present in phloem sap was defined, and several miRNAs and sRNAs were identified that are differentially expressed during iron deficiency.
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spelling pubmed-104211562023-08-12 The Small RNA Component of Arabidopsis thaliana Phloem Sap and Its Response to Iron Deficiency Bakirbas, Ahmet Castro-Rodriguez, Rosario Walker, Elsbeth L. Plants (Basel) Article In order to discover sRNA that might function during iron deficiency stress, RNA was prepared from phloem exudates of Arabidopsis thaliana, and used for RNA-seq. Bioanalyzer results indicate that abundant RNA from phloem is small in size—less than 200 nt. Moreover, typical rRNA bands were not observed. Sequencing of eight independent phloem RNA samples indicated that tRNA-derived fragments, specifically 5′ tRFs and 5′ tRNA halves, are highly abundant in phloem sap, comprising about 46% of all reads. In addition, a set of miRNAs that are present in phloem sap was defined, and several miRNAs and sRNAs were identified that are differentially expressed during iron deficiency. MDPI 2023-07-27 /pmc/articles/PMC10421156/ /pubmed/37570935 http://dx.doi.org/10.3390/plants12152782 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bakirbas, Ahmet
Castro-Rodriguez, Rosario
Walker, Elsbeth L.
The Small RNA Component of Arabidopsis thaliana Phloem Sap and Its Response to Iron Deficiency
title The Small RNA Component of Arabidopsis thaliana Phloem Sap and Its Response to Iron Deficiency
title_full The Small RNA Component of Arabidopsis thaliana Phloem Sap and Its Response to Iron Deficiency
title_fullStr The Small RNA Component of Arabidopsis thaliana Phloem Sap and Its Response to Iron Deficiency
title_full_unstemmed The Small RNA Component of Arabidopsis thaliana Phloem Sap and Its Response to Iron Deficiency
title_short The Small RNA Component of Arabidopsis thaliana Phloem Sap and Its Response to Iron Deficiency
title_sort small rna component of arabidopsis thaliana phloem sap and its response to iron deficiency
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421156/
https://www.ncbi.nlm.nih.gov/pubmed/37570935
http://dx.doi.org/10.3390/plants12152782
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