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Nitrile-Specific Protein NSP2 and Its Interacting Protein MPK3 Synergistically Regulate Plant Disease Resistance in Arabidopsis

Glucosinolates and their degradation products have a wide range of actions and are important components of plant defense. NSP2 (nitrile-specific protein 2) is a key regulator in the breakdown process of glucosinolates. However, the precise function of NSP2 in plant disease resistance beyond its role...

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Autores principales: Zhai, Tingting, Teng, Jun, Fan, Xintong, Yu, Shaowei, Wang, Chen, Guo, Xingqi, Yang, Wei, Zhang, Shuxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421354/
https://www.ncbi.nlm.nih.gov/pubmed/37571011
http://dx.doi.org/10.3390/plants12152857
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author Zhai, Tingting
Teng, Jun
Fan, Xintong
Yu, Shaowei
Wang, Chen
Guo, Xingqi
Yang, Wei
Zhang, Shuxin
author_facet Zhai, Tingting
Teng, Jun
Fan, Xintong
Yu, Shaowei
Wang, Chen
Guo, Xingqi
Yang, Wei
Zhang, Shuxin
author_sort Zhai, Tingting
collection PubMed
description Glucosinolates and their degradation products have a wide range of actions and are important components of plant defense. NSP2 (nitrile-specific protein 2) is a key regulator in the breakdown process of glucosinolates. However, the precise function of NSP2 in plant disease resistance beyond its role in glucosinolate degradation is still unclear. In this study, we discovered that NSP2 which was induced by Pst DC3000, influenced PR genes expression and reactive oxygen burst. Additionally, omics analysis revealed that NSP2 was engaged in plant-pathogen interaction and several hormone signal transduction pathways. Furthermore, immunoprecipitation-tandem mass spectrometry analysis (IP-MS), bimolecular fluorescence complementation (BiFC), and co-immunoprecipitation demonstrated that NSP2 interacts with MPK3. Genetic analysis shows that NSP2 may be a function downstream of MPK3. Upon pathogen inoculation, NSP2 protein levels increase while MPK3 protein levels decrease. Moreover, the level of phosphorylated NSP2 decreases. Taken together, this study sheds light on a new mode of synergistic action between NSP2 and MPK3 in the disease resistance process.
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spelling pubmed-104213542023-08-12 Nitrile-Specific Protein NSP2 and Its Interacting Protein MPK3 Synergistically Regulate Plant Disease Resistance in Arabidopsis Zhai, Tingting Teng, Jun Fan, Xintong Yu, Shaowei Wang, Chen Guo, Xingqi Yang, Wei Zhang, Shuxin Plants (Basel) Article Glucosinolates and their degradation products have a wide range of actions and are important components of plant defense. NSP2 (nitrile-specific protein 2) is a key regulator in the breakdown process of glucosinolates. However, the precise function of NSP2 in plant disease resistance beyond its role in glucosinolate degradation is still unclear. In this study, we discovered that NSP2 which was induced by Pst DC3000, influenced PR genes expression and reactive oxygen burst. Additionally, omics analysis revealed that NSP2 was engaged in plant-pathogen interaction and several hormone signal transduction pathways. Furthermore, immunoprecipitation-tandem mass spectrometry analysis (IP-MS), bimolecular fluorescence complementation (BiFC), and co-immunoprecipitation demonstrated that NSP2 interacts with MPK3. Genetic analysis shows that NSP2 may be a function downstream of MPK3. Upon pathogen inoculation, NSP2 protein levels increase while MPK3 protein levels decrease. Moreover, the level of phosphorylated NSP2 decreases. Taken together, this study sheds light on a new mode of synergistic action between NSP2 and MPK3 in the disease resistance process. MDPI 2023-08-03 /pmc/articles/PMC10421354/ /pubmed/37571011 http://dx.doi.org/10.3390/plants12152857 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhai, Tingting
Teng, Jun
Fan, Xintong
Yu, Shaowei
Wang, Chen
Guo, Xingqi
Yang, Wei
Zhang, Shuxin
Nitrile-Specific Protein NSP2 and Its Interacting Protein MPK3 Synergistically Regulate Plant Disease Resistance in Arabidopsis
title Nitrile-Specific Protein NSP2 and Its Interacting Protein MPK3 Synergistically Regulate Plant Disease Resistance in Arabidopsis
title_full Nitrile-Specific Protein NSP2 and Its Interacting Protein MPK3 Synergistically Regulate Plant Disease Resistance in Arabidopsis
title_fullStr Nitrile-Specific Protein NSP2 and Its Interacting Protein MPK3 Synergistically Regulate Plant Disease Resistance in Arabidopsis
title_full_unstemmed Nitrile-Specific Protein NSP2 and Its Interacting Protein MPK3 Synergistically Regulate Plant Disease Resistance in Arabidopsis
title_short Nitrile-Specific Protein NSP2 and Its Interacting Protein MPK3 Synergistically Regulate Plant Disease Resistance in Arabidopsis
title_sort nitrile-specific protein nsp2 and its interacting protein mpk3 synergistically regulate plant disease resistance in arabidopsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421354/
https://www.ncbi.nlm.nih.gov/pubmed/37571011
http://dx.doi.org/10.3390/plants12152857
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