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Electrospun Scaffolds Enriched with Nanoparticle-Associated DNA: General Properties, DNA Release and Cell Transfection

Biomaterial-mediated, spatially localized gene delivery is important for the development of cell-populated scaffolds used in tissue engineering. Cells adhering to or penetrating into such a scaffold are to be transfected with a preloaded gene that induces the production of secreted proteins or cell...

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Autores principales: Chernonosova, Vera, Khlebnikova, Marianna, Popova, Victoriya, Starostina, Ekaterina, Kiseleva, Elena, Chelobanov, Boris, Kvon, Ren, Dmitrienko, Elena, Laktionov, Pavel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421399/
https://www.ncbi.nlm.nih.gov/pubmed/37571096
http://dx.doi.org/10.3390/polym15153202
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author Chernonosova, Vera
Khlebnikova, Marianna
Popova, Victoriya
Starostina, Ekaterina
Kiseleva, Elena
Chelobanov, Boris
Kvon, Ren
Dmitrienko, Elena
Laktionov, Pavel
author_facet Chernonosova, Vera
Khlebnikova, Marianna
Popova, Victoriya
Starostina, Ekaterina
Kiseleva, Elena
Chelobanov, Boris
Kvon, Ren
Dmitrienko, Elena
Laktionov, Pavel
author_sort Chernonosova, Vera
collection PubMed
description Biomaterial-mediated, spatially localized gene delivery is important for the development of cell-populated scaffolds used in tissue engineering. Cells adhering to or penetrating into such a scaffold are to be transfected with a preloaded gene that induces the production of secreted proteins or cell reprogramming. In the present study, we produced silica nanoparticles-associated pDNA and electrospun scaffolds loaded with such nanoparticles, and studied the release of pDNA from scaffolds and cell-to-scaffold interactions in terms of cell viability and pDNA transfection efficacy. The pDNA-coated nanoparticles were characterized with dynamic light scattering and transmission electron microscopy. Particle sizes ranging from 56 to 78 nm were indicative of their potential for cell transfection. The scaffolds were characterized using scanning electron microscopy, X-ray photoelectron spectroscopy, stress-loading tests and interaction with HEK293T cells. It was found that the properties of materials and the pDNA released vary, depending on the scaffold’s composition. The scaffolds loaded with pDNA-nanoparticles do not have a pronounced cytotoxic effect, and can be recommended for cell transfection. It was found that (pDNA-NPs) + PEI9-loaded scaffold demonstrates good potential for cell transfection. Thus, electrospun scaffolds suitable for the transfection of inhabiting cells are eligible for use in tissue engineering.
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spelling pubmed-104213992023-08-12 Electrospun Scaffolds Enriched with Nanoparticle-Associated DNA: General Properties, DNA Release and Cell Transfection Chernonosova, Vera Khlebnikova, Marianna Popova, Victoriya Starostina, Ekaterina Kiseleva, Elena Chelobanov, Boris Kvon, Ren Dmitrienko, Elena Laktionov, Pavel Polymers (Basel) Article Biomaterial-mediated, spatially localized gene delivery is important for the development of cell-populated scaffolds used in tissue engineering. Cells adhering to or penetrating into such a scaffold are to be transfected with a preloaded gene that induces the production of secreted proteins or cell reprogramming. In the present study, we produced silica nanoparticles-associated pDNA and electrospun scaffolds loaded with such nanoparticles, and studied the release of pDNA from scaffolds and cell-to-scaffold interactions in terms of cell viability and pDNA transfection efficacy. The pDNA-coated nanoparticles were characterized with dynamic light scattering and transmission electron microscopy. Particle sizes ranging from 56 to 78 nm were indicative of their potential for cell transfection. The scaffolds were characterized using scanning electron microscopy, X-ray photoelectron spectroscopy, stress-loading tests and interaction with HEK293T cells. It was found that the properties of materials and the pDNA released vary, depending on the scaffold’s composition. The scaffolds loaded with pDNA-nanoparticles do not have a pronounced cytotoxic effect, and can be recommended for cell transfection. It was found that (pDNA-NPs) + PEI9-loaded scaffold demonstrates good potential for cell transfection. Thus, electrospun scaffolds suitable for the transfection of inhabiting cells are eligible for use in tissue engineering. MDPI 2023-07-27 /pmc/articles/PMC10421399/ /pubmed/37571096 http://dx.doi.org/10.3390/polym15153202 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chernonosova, Vera
Khlebnikova, Marianna
Popova, Victoriya
Starostina, Ekaterina
Kiseleva, Elena
Chelobanov, Boris
Kvon, Ren
Dmitrienko, Elena
Laktionov, Pavel
Electrospun Scaffolds Enriched with Nanoparticle-Associated DNA: General Properties, DNA Release and Cell Transfection
title Electrospun Scaffolds Enriched with Nanoparticle-Associated DNA: General Properties, DNA Release and Cell Transfection
title_full Electrospun Scaffolds Enriched with Nanoparticle-Associated DNA: General Properties, DNA Release and Cell Transfection
title_fullStr Electrospun Scaffolds Enriched with Nanoparticle-Associated DNA: General Properties, DNA Release and Cell Transfection
title_full_unstemmed Electrospun Scaffolds Enriched with Nanoparticle-Associated DNA: General Properties, DNA Release and Cell Transfection
title_short Electrospun Scaffolds Enriched with Nanoparticle-Associated DNA: General Properties, DNA Release and Cell Transfection
title_sort electrospun scaffolds enriched with nanoparticle-associated dna: general properties, dna release and cell transfection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421399/
https://www.ncbi.nlm.nih.gov/pubmed/37571096
http://dx.doi.org/10.3390/polym15153202
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