Cargando…

Fabrication and Validation of a 3D Portable PEGDA Microfluidic Chip for Visual Colorimetric Detection of Captured Breast Cancer Cells

To guide therapeutic strategies and to monitor the state changes in the disease, a low-cost, portable, and easily fabricated microfluidic-chip-integrated three-dimensional (3D) microchamber was designed for capturing and analyzing breast cancer cells. Optimally, a colorimetric sensor array was integ...

Descripción completa

Detalles Bibliográficos
Autores principales: Guo, Mingyi, Deng, Yan, Huang, Junqiu, Huang, Yanping, Deng, Jing, Wu, Huachang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421435/
https://www.ncbi.nlm.nih.gov/pubmed/37571077
http://dx.doi.org/10.3390/polym15153183
_version_ 1785088979631079424
author Guo, Mingyi
Deng, Yan
Huang, Junqiu
Huang, Yanping
Deng, Jing
Wu, Huachang
author_facet Guo, Mingyi
Deng, Yan
Huang, Junqiu
Huang, Yanping
Deng, Jing
Wu, Huachang
author_sort Guo, Mingyi
collection PubMed
description To guide therapeutic strategies and to monitor the state changes in the disease, a low-cost, portable, and easily fabricated microfluidic-chip-integrated three-dimensional (3D) microchamber was designed for capturing and analyzing breast cancer cells. Optimally, a colorimetric sensor array was integrated into a microfluidic chip to discriminate the metabolites of the cells. The ultraviolet polymerization characteristic of poly(ethylene glycol) diacrylate (PEGDA) hydrogel was utilized to rapidly fabricate a three-layer hydrogel microfluidic chip with the designed structure under noninvasive 365 nm laser irradiation. 2-Hydroxyethyl methacrylate (HEMA) was added to the prepolymer in order to increase the adhesive capacity of the microchip’s surface for capturing cells. 1-Vinyl-2-pyrrolidone (NVP) was designed to improve the toughness and reduce the swelling capacity of the hydrogel composite. A non-toxic 3D hydrogel microarray chip (60 mm × 20 mm × 3 mm) with low immunogenicity and high hydrophilicity was created to simulate the real physiological microenvironment of breast tissue. The crisscross channels were designed to ensure homogeneous seeding density. This hydrogel material displayed excellent biocompatibility and tunable physical properties compared with traditional microfluidic chip materials and can be directly processed to obtain the most desirable microstructure. The feasibility of using a PEGDA hydrogel microfluidic chip for the real-time online detection of breast cancer cells’ metabolism was confirmed using a specifically designed colorimetric sensor array with 16 kinds of porphyrin, porphyrin derivatives, and indicator dyes. The results of the principal component analysis (PCA), the hierarchical cluster analysis (HCA), and the linear discriminant analysis (LDA) suggest that the metabolic liquids of different breast cells can be easily distinguished with the developed PEGDA hydrogel microfluidic chip. The PEGDA hydrogel microfluidic chip has potential practicable applicability in distinguishing normal and cancerous breast cells.
format Online
Article
Text
id pubmed-10421435
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-104214352023-08-12 Fabrication and Validation of a 3D Portable PEGDA Microfluidic Chip for Visual Colorimetric Detection of Captured Breast Cancer Cells Guo, Mingyi Deng, Yan Huang, Junqiu Huang, Yanping Deng, Jing Wu, Huachang Polymers (Basel) Article To guide therapeutic strategies and to monitor the state changes in the disease, a low-cost, portable, and easily fabricated microfluidic-chip-integrated three-dimensional (3D) microchamber was designed for capturing and analyzing breast cancer cells. Optimally, a colorimetric sensor array was integrated into a microfluidic chip to discriminate the metabolites of the cells. The ultraviolet polymerization characteristic of poly(ethylene glycol) diacrylate (PEGDA) hydrogel was utilized to rapidly fabricate a three-layer hydrogel microfluidic chip with the designed structure under noninvasive 365 nm laser irradiation. 2-Hydroxyethyl methacrylate (HEMA) was added to the prepolymer in order to increase the adhesive capacity of the microchip’s surface for capturing cells. 1-Vinyl-2-pyrrolidone (NVP) was designed to improve the toughness and reduce the swelling capacity of the hydrogel composite. A non-toxic 3D hydrogel microarray chip (60 mm × 20 mm × 3 mm) with low immunogenicity and high hydrophilicity was created to simulate the real physiological microenvironment of breast tissue. The crisscross channels were designed to ensure homogeneous seeding density. This hydrogel material displayed excellent biocompatibility and tunable physical properties compared with traditional microfluidic chip materials and can be directly processed to obtain the most desirable microstructure. The feasibility of using a PEGDA hydrogel microfluidic chip for the real-time online detection of breast cancer cells’ metabolism was confirmed using a specifically designed colorimetric sensor array with 16 kinds of porphyrin, porphyrin derivatives, and indicator dyes. The results of the principal component analysis (PCA), the hierarchical cluster analysis (HCA), and the linear discriminant analysis (LDA) suggest that the metabolic liquids of different breast cells can be easily distinguished with the developed PEGDA hydrogel microfluidic chip. The PEGDA hydrogel microfluidic chip has potential practicable applicability in distinguishing normal and cancerous breast cells. MDPI 2023-07-27 /pmc/articles/PMC10421435/ /pubmed/37571077 http://dx.doi.org/10.3390/polym15153183 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guo, Mingyi
Deng, Yan
Huang, Junqiu
Huang, Yanping
Deng, Jing
Wu, Huachang
Fabrication and Validation of a 3D Portable PEGDA Microfluidic Chip for Visual Colorimetric Detection of Captured Breast Cancer Cells
title Fabrication and Validation of a 3D Portable PEGDA Microfluidic Chip for Visual Colorimetric Detection of Captured Breast Cancer Cells
title_full Fabrication and Validation of a 3D Portable PEGDA Microfluidic Chip for Visual Colorimetric Detection of Captured Breast Cancer Cells
title_fullStr Fabrication and Validation of a 3D Portable PEGDA Microfluidic Chip for Visual Colorimetric Detection of Captured Breast Cancer Cells
title_full_unstemmed Fabrication and Validation of a 3D Portable PEGDA Microfluidic Chip for Visual Colorimetric Detection of Captured Breast Cancer Cells
title_short Fabrication and Validation of a 3D Portable PEGDA Microfluidic Chip for Visual Colorimetric Detection of Captured Breast Cancer Cells
title_sort fabrication and validation of a 3d portable pegda microfluidic chip for visual colorimetric detection of captured breast cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421435/
https://www.ncbi.nlm.nih.gov/pubmed/37571077
http://dx.doi.org/10.3390/polym15153183
work_keys_str_mv AT guomingyi fabricationandvalidationofa3dportablepegdamicrofluidicchipforvisualcolorimetricdetectionofcapturedbreastcancercells
AT dengyan fabricationandvalidationofa3dportablepegdamicrofluidicchipforvisualcolorimetricdetectionofcapturedbreastcancercells
AT huangjunqiu fabricationandvalidationofa3dportablepegdamicrofluidicchipforvisualcolorimetricdetectionofcapturedbreastcancercells
AT huangyanping fabricationandvalidationofa3dportablepegdamicrofluidicchipforvisualcolorimetricdetectionofcapturedbreastcancercells
AT dengjing fabricationandvalidationofa3dportablepegdamicrofluidicchipforvisualcolorimetricdetectionofcapturedbreastcancercells
AT wuhuachang fabricationandvalidationofa3dportablepegdamicrofluidicchipforvisualcolorimetricdetectionofcapturedbreastcancercells