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First report of mobile colistin resistance gene mcr-1 in avian pathogenic Escherichia coli isolated from turkeys in the Gaza Strip, Palestine

BACKGROUND AND AIM: Colistin is used to treat avian pathogenic Escherichia coli (APEC), a microorganism that affects turkey meat production in the Gaza Strip and worldwide. However, the recent emergence of plasmid-borne mobile colistin resistance (mcr) genes in pathogenic E. coli strains is a seriou...

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Autores principales: Thabet, Ahmed M., Alzuheir, Ibrahim M., Laham, Nahed A. Al, Helal, Belal Y. Abu, Fayyad, Adnan F., Jalboush, Nasr H., Gharaibeh, Mohammad H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421552/
https://www.ncbi.nlm.nih.gov/pubmed/37577205
http://dx.doi.org/10.14202/vetworld.2023.1260-1265
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author Thabet, Ahmed M.
Alzuheir, Ibrahim M.
Laham, Nahed A. Al
Helal, Belal Y. Abu
Fayyad, Adnan F.
Jalboush, Nasr H.
Gharaibeh, Mohammad H.
author_facet Thabet, Ahmed M.
Alzuheir, Ibrahim M.
Laham, Nahed A. Al
Helal, Belal Y. Abu
Fayyad, Adnan F.
Jalboush, Nasr H.
Gharaibeh, Mohammad H.
author_sort Thabet, Ahmed M.
collection PubMed
description BACKGROUND AND AIM: Colistin is used to treat avian pathogenic Escherichia coli (APEC), a microorganism that affects turkey meat production in the Gaza Strip and worldwide. However, the recent emergence of plasmid-borne mobile colistin resistance (mcr) genes in pathogenic E. coli strains is a serious antimicrobial resistance (AMR) challenge for both human and animal health. In December 2018, colistin was banned as a veterinary antimicrobial in the Gaza Strip. This study aimed to detect and track the prevalence of colistin-resistant APEC isolated from turkey flocks in the Gaza Strip. MATERIALS AND METHODS: This study investigated 239 APEC isolates from turkey flocks in the Gaza Strip between October 2018 and December 2021 (at 6-month intervals). The colistin-resistant APEC strains were detected using the broth microdilution method. The mcr-1 gene was identified using a polymerase chain reaction. RESULTS: The overall colistin resistance among the isolated APECs was 32.2% during the study period. The average resistance in the first interval was 37.5%, which significantly decreased to 9.3% in the last interval. Among the 77 phenotypically resistant isolates, 32.4% were positive for mcr-1. The average abundance of mcr-1 in the first interval was 66.6%, which decreased to 25% in the last interval. CONCLUSION: To the best of our knowledge, this is the first study reporting the presence of the mcr-1 gene among the APEC isolates from turkeys in the Gaza Strip. Banned veterinary use of colistin significantly decreased the percentage of resistant APEC isolates from turkeys in Gaza Strip. Further studies are needed to investigate other colistin resistance genes and track the emergence of AMR.
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spelling pubmed-104215522023-08-12 First report of mobile colistin resistance gene mcr-1 in avian pathogenic Escherichia coli isolated from turkeys in the Gaza Strip, Palestine Thabet, Ahmed M. Alzuheir, Ibrahim M. Laham, Nahed A. Al Helal, Belal Y. Abu Fayyad, Adnan F. Jalboush, Nasr H. Gharaibeh, Mohammad H. Vet World Research Article BACKGROUND AND AIM: Colistin is used to treat avian pathogenic Escherichia coli (APEC), a microorganism that affects turkey meat production in the Gaza Strip and worldwide. However, the recent emergence of plasmid-borne mobile colistin resistance (mcr) genes in pathogenic E. coli strains is a serious antimicrobial resistance (AMR) challenge for both human and animal health. In December 2018, colistin was banned as a veterinary antimicrobial in the Gaza Strip. This study aimed to detect and track the prevalence of colistin-resistant APEC isolated from turkey flocks in the Gaza Strip. MATERIALS AND METHODS: This study investigated 239 APEC isolates from turkey flocks in the Gaza Strip between October 2018 and December 2021 (at 6-month intervals). The colistin-resistant APEC strains were detected using the broth microdilution method. The mcr-1 gene was identified using a polymerase chain reaction. RESULTS: The overall colistin resistance among the isolated APECs was 32.2% during the study period. The average resistance in the first interval was 37.5%, which significantly decreased to 9.3% in the last interval. Among the 77 phenotypically resistant isolates, 32.4% were positive for mcr-1. The average abundance of mcr-1 in the first interval was 66.6%, which decreased to 25% in the last interval. CONCLUSION: To the best of our knowledge, this is the first study reporting the presence of the mcr-1 gene among the APEC isolates from turkeys in the Gaza Strip. Banned veterinary use of colistin significantly decreased the percentage of resistant APEC isolates from turkeys in Gaza Strip. Further studies are needed to investigate other colistin resistance genes and track the emergence of AMR. Veterinary World 2023-06 2023-06-08 /pmc/articles/PMC10421552/ /pubmed/37577205 http://dx.doi.org/10.14202/vetworld.2023.1260-1265 Text en Copyright: © Thabet, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Thabet, Ahmed M.
Alzuheir, Ibrahim M.
Laham, Nahed A. Al
Helal, Belal Y. Abu
Fayyad, Adnan F.
Jalboush, Nasr H.
Gharaibeh, Mohammad H.
First report of mobile colistin resistance gene mcr-1 in avian pathogenic Escherichia coli isolated from turkeys in the Gaza Strip, Palestine
title First report of mobile colistin resistance gene mcr-1 in avian pathogenic Escherichia coli isolated from turkeys in the Gaza Strip, Palestine
title_full First report of mobile colistin resistance gene mcr-1 in avian pathogenic Escherichia coli isolated from turkeys in the Gaza Strip, Palestine
title_fullStr First report of mobile colistin resistance gene mcr-1 in avian pathogenic Escherichia coli isolated from turkeys in the Gaza Strip, Palestine
title_full_unstemmed First report of mobile colistin resistance gene mcr-1 in avian pathogenic Escherichia coli isolated from turkeys in the Gaza Strip, Palestine
title_short First report of mobile colistin resistance gene mcr-1 in avian pathogenic Escherichia coli isolated from turkeys in the Gaza Strip, Palestine
title_sort first report of mobile colistin resistance gene mcr-1 in avian pathogenic escherichia coli isolated from turkeys in the gaza strip, palestine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421552/
https://www.ncbi.nlm.nih.gov/pubmed/37577205
http://dx.doi.org/10.14202/vetworld.2023.1260-1265
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