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Open AccessLack of association between coat color abnormalities in Bali cattle (Bos javanicus) and the coding regions of the MC1R and KIT genes

BACKGROUND AND AIM: Coat color variations in cattle are known to be influenced by the melanocortin 1 receptor (MC1R) and receptor tyrosine kinase (KIT) genes. The presence of coat color abnormalities, such as white spots and albinism, in Bali cattle was the focus of this study. This study aimed to i...

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Autores principales: Jakaria, Jakaria, Kholijah, Kholijah, Darwati, Sri, Rahman, Qonita, Daulay, Winni Liani, Suhendro, Ikhsan, Londra, I. Made, Ulum, Mokhamad Fakhrul, Noor, Ronny Rachman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421557/
https://www.ncbi.nlm.nih.gov/pubmed/37577199
http://dx.doi.org/10.14202/vetworld.2023.1312-1318
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author Jakaria, Jakaria
Kholijah, Kholijah
Darwati, Sri
Rahman, Qonita
Daulay, Winni Liani
Suhendro, Ikhsan
Londra, I. Made
Ulum, Mokhamad Fakhrul
Noor, Ronny Rachman
author_facet Jakaria, Jakaria
Kholijah, Kholijah
Darwati, Sri
Rahman, Qonita
Daulay, Winni Liani
Suhendro, Ikhsan
Londra, I. Made
Ulum, Mokhamad Fakhrul
Noor, Ronny Rachman
author_sort Jakaria, Jakaria
collection PubMed
description BACKGROUND AND AIM: Coat color variations in cattle are known to be influenced by the melanocortin 1 receptor (MC1R) and receptor tyrosine kinase (KIT) genes. The presence of coat color abnormalities, such as white spots and albinism, in Bali cattle was the focus of this study. This study aimed to identify single nucleotide polymorphisms (SNPs) in the coding region of MC1R and exons 2 and 3 of KIT associated with coat color abnormalities in Bali cattle. MATERIALS AND METHODS: The study included the analysis of 48 Bali cattle, including 20 individuals with standard coat color, 15 with white spots, and 13 with albinism. Total DNA was extracted using a DNA Extraction Kit, and MC1R (coding region) and KIT (exons 2 and 3) gene amplifications were analyzed using forward and reverse primers with polymerase chain reaction product lengths of 1071, 234, and 448 bp, respectively. The determination of MC1R and KIT gene diversity was analyzed through direct sequencing. Melanocortin 1 receptor and KIT gene sequence data were analyzed using BioEdit and MEGA6 to identify SNPs associated with standard and abnormal coat color phenotypes (white-spotted and albino) in Bali cattle. RESULTS: No SNPs associated with coat color abnormalities were found in the coding region of MC1R and exons 2 and 3 of KIT genes in Bali cattle. However, the intron two regions of KIT contained the SNP g.70208534A>G, which showed a high degree of diversity. The AA genotype frequency was highest in albino Bali cattle, whereas the G allele frequency was highest and the A allele frequency was lowest in white-spotted Bali cattle. CONCLUSION: The results indicated that standard, white-spotted, and albino coat colors in Bali cattle could not be distinguished by analyzing the MC1R and KIT genes.
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spelling pubmed-104215572023-08-12 Open AccessLack of association between coat color abnormalities in Bali cattle (Bos javanicus) and the coding regions of the MC1R and KIT genes Jakaria, Jakaria Kholijah, Kholijah Darwati, Sri Rahman, Qonita Daulay, Winni Liani Suhendro, Ikhsan Londra, I. Made Ulum, Mokhamad Fakhrul Noor, Ronny Rachman Vet World Research Article BACKGROUND AND AIM: Coat color variations in cattle are known to be influenced by the melanocortin 1 receptor (MC1R) and receptor tyrosine kinase (KIT) genes. The presence of coat color abnormalities, such as white spots and albinism, in Bali cattle was the focus of this study. This study aimed to identify single nucleotide polymorphisms (SNPs) in the coding region of MC1R and exons 2 and 3 of KIT associated with coat color abnormalities in Bali cattle. MATERIALS AND METHODS: The study included the analysis of 48 Bali cattle, including 20 individuals with standard coat color, 15 with white spots, and 13 with albinism. Total DNA was extracted using a DNA Extraction Kit, and MC1R (coding region) and KIT (exons 2 and 3) gene amplifications were analyzed using forward and reverse primers with polymerase chain reaction product lengths of 1071, 234, and 448 bp, respectively. The determination of MC1R and KIT gene diversity was analyzed through direct sequencing. Melanocortin 1 receptor and KIT gene sequence data were analyzed using BioEdit and MEGA6 to identify SNPs associated with standard and abnormal coat color phenotypes (white-spotted and albino) in Bali cattle. RESULTS: No SNPs associated with coat color abnormalities were found in the coding region of MC1R and exons 2 and 3 of KIT genes in Bali cattle. However, the intron two regions of KIT contained the SNP g.70208534A>G, which showed a high degree of diversity. The AA genotype frequency was highest in albino Bali cattle, whereas the G allele frequency was highest and the A allele frequency was lowest in white-spotted Bali cattle. CONCLUSION: The results indicated that standard, white-spotted, and albino coat colors in Bali cattle could not be distinguished by analyzing the MC1R and KIT genes. Veterinary World 2023-06 2023-06-13 /pmc/articles/PMC10421557/ /pubmed/37577199 http://dx.doi.org/10.14202/vetworld.2023.1312-1318 Text en Copyright: © Jakaria, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Jakaria, Jakaria
Kholijah, Kholijah
Darwati, Sri
Rahman, Qonita
Daulay, Winni Liani
Suhendro, Ikhsan
Londra, I. Made
Ulum, Mokhamad Fakhrul
Noor, Ronny Rachman
Open AccessLack of association between coat color abnormalities in Bali cattle (Bos javanicus) and the coding regions of the MC1R and KIT genes
title Open AccessLack of association between coat color abnormalities in Bali cattle (Bos javanicus) and the coding regions of the MC1R and KIT genes
title_full Open AccessLack of association between coat color abnormalities in Bali cattle (Bos javanicus) and the coding regions of the MC1R and KIT genes
title_fullStr Open AccessLack of association between coat color abnormalities in Bali cattle (Bos javanicus) and the coding regions of the MC1R and KIT genes
title_full_unstemmed Open AccessLack of association between coat color abnormalities in Bali cattle (Bos javanicus) and the coding regions of the MC1R and KIT genes
title_short Open AccessLack of association between coat color abnormalities in Bali cattle (Bos javanicus) and the coding regions of the MC1R and KIT genes
title_sort open accesslack of association between coat color abnormalities in bali cattle (bos javanicus) and the coding regions of the mc1r and kit genes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421557/
https://www.ncbi.nlm.nih.gov/pubmed/37577199
http://dx.doi.org/10.14202/vetworld.2023.1312-1318
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