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ECS1 and ECS2 suppress polyspermy and the formation of haploid plants by promoting double fertilization
The current pace of crop plant optimization is insufficient to meet future demands and there is an urgent need for novel breeding strategies. It was previously shown that plants tolerate the generation of triparental polyspermy-derived plants and that polyspermy can bypass hybridization barriers. Po...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421590/ https://www.ncbi.nlm.nih.gov/pubmed/37489742 http://dx.doi.org/10.7554/eLife.85832 |
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author | Mao, Yanbo Nakel, Thomas Erbasol Serbes, Isil Joshi, Saurabh Tekleyohans, Dawit G Baum, Thomas Groß-Hardt, Rita |
author_facet | Mao, Yanbo Nakel, Thomas Erbasol Serbes, Isil Joshi, Saurabh Tekleyohans, Dawit G Baum, Thomas Groß-Hardt, Rita |
author_sort | Mao, Yanbo |
collection | PubMed |
description | The current pace of crop plant optimization is insufficient to meet future demands and there is an urgent need for novel breeding strategies. It was previously shown that plants tolerate the generation of triparental polyspermy-derived plants and that polyspermy can bypass hybridization barriers. Polyspermy thus has the potential to harness previously incompatible climate-adapted wild varieties for plant breeding. However, factors that influence polyspermy frequencies were not previously known. The endopeptidases ECS1 and ECS2 have been reported to prevent the attraction of supernumerary pollen tubes by cleaving the pollen tube attractant LURE1. Here, we show that these genes have an earlier function that is manifested by incomplete double fertilization in plants defective for both genes. In addition to supernumerary pollen tube attraction, ecs1 ecs2 mutants exhibit a delay in synergid disintegration, are susceptible to heterofertilization, and segregate haploid plants that lack a paternal genome contribution. Our results thus uncover ECS1 and ECS2 as the first female factors triggering the induction of maternal haploids. Capitalizing on a high-throughput polyspermy assay, we in addition show that the double mutant exhibits an increase in polyspermy frequencies. As both haploid induction and polyspermy are valuable breeding aims, our results open new avenues for accelerated generation of climate-adapted cultivars. |
format | Online Article Text |
id | pubmed-10421590 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-104215902023-08-12 ECS1 and ECS2 suppress polyspermy and the formation of haploid plants by promoting double fertilization Mao, Yanbo Nakel, Thomas Erbasol Serbes, Isil Joshi, Saurabh Tekleyohans, Dawit G Baum, Thomas Groß-Hardt, Rita eLife Developmental Biology The current pace of crop plant optimization is insufficient to meet future demands and there is an urgent need for novel breeding strategies. It was previously shown that plants tolerate the generation of triparental polyspermy-derived plants and that polyspermy can bypass hybridization barriers. Polyspermy thus has the potential to harness previously incompatible climate-adapted wild varieties for plant breeding. However, factors that influence polyspermy frequencies were not previously known. The endopeptidases ECS1 and ECS2 have been reported to prevent the attraction of supernumerary pollen tubes by cleaving the pollen tube attractant LURE1. Here, we show that these genes have an earlier function that is manifested by incomplete double fertilization in plants defective for both genes. In addition to supernumerary pollen tube attraction, ecs1 ecs2 mutants exhibit a delay in synergid disintegration, are susceptible to heterofertilization, and segregate haploid plants that lack a paternal genome contribution. Our results thus uncover ECS1 and ECS2 as the first female factors triggering the induction of maternal haploids. Capitalizing on a high-throughput polyspermy assay, we in addition show that the double mutant exhibits an increase in polyspermy frequencies. As both haploid induction and polyspermy are valuable breeding aims, our results open new avenues for accelerated generation of climate-adapted cultivars. eLife Sciences Publications, Ltd 2023-07-25 /pmc/articles/PMC10421590/ /pubmed/37489742 http://dx.doi.org/10.7554/eLife.85832 Text en © 2023, Mao, Nakel et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology Mao, Yanbo Nakel, Thomas Erbasol Serbes, Isil Joshi, Saurabh Tekleyohans, Dawit G Baum, Thomas Groß-Hardt, Rita ECS1 and ECS2 suppress polyspermy and the formation of haploid plants by promoting double fertilization |
title | ECS1 and ECS2 suppress polyspermy and the formation of haploid plants by promoting double fertilization |
title_full | ECS1 and ECS2 suppress polyspermy and the formation of haploid plants by promoting double fertilization |
title_fullStr | ECS1 and ECS2 suppress polyspermy and the formation of haploid plants by promoting double fertilization |
title_full_unstemmed | ECS1 and ECS2 suppress polyspermy and the formation of haploid plants by promoting double fertilization |
title_short | ECS1 and ECS2 suppress polyspermy and the formation of haploid plants by promoting double fertilization |
title_sort | ecs1 and ecs2 suppress polyspermy and the formation of haploid plants by promoting double fertilization |
topic | Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421590/ https://www.ncbi.nlm.nih.gov/pubmed/37489742 http://dx.doi.org/10.7554/eLife.85832 |
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