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Rhizosphere frame system enables nondestructive live-imaging of legume-rhizobium interactions in the soil

Most plants interact with various soil microorganisms as they grow through the soil. Root nodule symbiosis by legumes and rhizobia is a well-known phenomenon of plant–microbe interactions in the soil. Although microscopic observations are useful for understanding the infection processes of rhizobia,...

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Autores principales: Nishida, Hanna, Shimoda, Yoshikazu, Win, Khin Thuzar, Imaizumi-Anraku, Haruko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Nature Singapore 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421814/
https://www.ncbi.nlm.nih.gov/pubmed/37402088
http://dx.doi.org/10.1007/s10265-023-01476-2
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author Nishida, Hanna
Shimoda, Yoshikazu
Win, Khin Thuzar
Imaizumi-Anraku, Haruko
author_facet Nishida, Hanna
Shimoda, Yoshikazu
Win, Khin Thuzar
Imaizumi-Anraku, Haruko
author_sort Nishida, Hanna
collection PubMed
description Most plants interact with various soil microorganisms as they grow through the soil. Root nodule symbiosis by legumes and rhizobia is a well-known phenomenon of plant–microbe interactions in the soil. Although microscopic observations are useful for understanding the infection processes of rhizobia, nondestructive observation methods have not been established for monitoring interactions between rhizobia and soil-grown roots. In this study, we constructed Bradyrhizobium diazoefficiens strains that constitutively express different fluorescent proteins, which allows identification of tagged rhizobia by the type of fluorophores. In addition, we constructed a plant cultivation device, Rhizosphere Frame (RhizoFrame), which is a soil-filled container made of transparent acrylic plates that allows observation of roots growing along the acrylic plates. Combining fluorescent rhizobia with RhizoFrame, we established a live imaging system, RhizoFrame system, that enabled us to track the nodulation processes with fluorescence stereomicroscope while retaining spatial information about roots, rhizobia, and soil. Mixed inoculation with different fluorescent rhizobia using RhizoFrame enabled the visualization of mixed infection of a single nodule with two strains. In addition, observation of transgenic Lotus japonicus expressing auxin-responsive reporter genes indicated that RhizoFrame system could be used for a real-time and nondestructive reporter assay. Thus, the use of RhizoFrame system is expected to enhance the study of the spatiotemporal dynamics of plant–microbe interactions in the soil. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10265-023-01476-2.
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spelling pubmed-104218142023-08-13 Rhizosphere frame system enables nondestructive live-imaging of legume-rhizobium interactions in the soil Nishida, Hanna Shimoda, Yoshikazu Win, Khin Thuzar Imaizumi-Anraku, Haruko J Plant Res Technical Note Most plants interact with various soil microorganisms as they grow through the soil. Root nodule symbiosis by legumes and rhizobia is a well-known phenomenon of plant–microbe interactions in the soil. Although microscopic observations are useful for understanding the infection processes of rhizobia, nondestructive observation methods have not been established for monitoring interactions between rhizobia and soil-grown roots. In this study, we constructed Bradyrhizobium diazoefficiens strains that constitutively express different fluorescent proteins, which allows identification of tagged rhizobia by the type of fluorophores. In addition, we constructed a plant cultivation device, Rhizosphere Frame (RhizoFrame), which is a soil-filled container made of transparent acrylic plates that allows observation of roots growing along the acrylic plates. Combining fluorescent rhizobia with RhizoFrame, we established a live imaging system, RhizoFrame system, that enabled us to track the nodulation processes with fluorescence stereomicroscope while retaining spatial information about roots, rhizobia, and soil. Mixed inoculation with different fluorescent rhizobia using RhizoFrame enabled the visualization of mixed infection of a single nodule with two strains. In addition, observation of transgenic Lotus japonicus expressing auxin-responsive reporter genes indicated that RhizoFrame system could be used for a real-time and nondestructive reporter assay. Thus, the use of RhizoFrame system is expected to enhance the study of the spatiotemporal dynamics of plant–microbe interactions in the soil. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10265-023-01476-2. Springer Nature Singapore 2023-07-04 2023 /pmc/articles/PMC10421814/ /pubmed/37402088 http://dx.doi.org/10.1007/s10265-023-01476-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Technical Note
Nishida, Hanna
Shimoda, Yoshikazu
Win, Khin Thuzar
Imaizumi-Anraku, Haruko
Rhizosphere frame system enables nondestructive live-imaging of legume-rhizobium interactions in the soil
title Rhizosphere frame system enables nondestructive live-imaging of legume-rhizobium interactions in the soil
title_full Rhizosphere frame system enables nondestructive live-imaging of legume-rhizobium interactions in the soil
title_fullStr Rhizosphere frame system enables nondestructive live-imaging of legume-rhizobium interactions in the soil
title_full_unstemmed Rhizosphere frame system enables nondestructive live-imaging of legume-rhizobium interactions in the soil
title_short Rhizosphere frame system enables nondestructive live-imaging of legume-rhizobium interactions in the soil
title_sort rhizosphere frame system enables nondestructive live-imaging of legume-rhizobium interactions in the soil
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421814/
https://www.ncbi.nlm.nih.gov/pubmed/37402088
http://dx.doi.org/10.1007/s10265-023-01476-2
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