Cargando…

Subtelomeric 5-enolpyruvylshikimate-3-phosphate synthase copy number variation confers glyphosate resistance in Eleusine indica

Genomic structural variation (SV) has profound effects on organismal evolution; often serving as a source of novel genetic variation. Gene copy number variation (CNV), one type of SV, has repeatedly been associated with adaptive evolution in eukaryotes, especially with environmental stress. Resistan...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Chun, Johnson, Nicholas A., Hall, Nathan, Tian, Xingshan, Yu, Qin, Patterson, Eric L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421919/
https://www.ncbi.nlm.nih.gov/pubmed/37567866
http://dx.doi.org/10.1038/s41467-023-40407-6
_version_ 1785089083183202304
author Zhang, Chun
Johnson, Nicholas A.
Hall, Nathan
Tian, Xingshan
Yu, Qin
Patterson, Eric L.
author_facet Zhang, Chun
Johnson, Nicholas A.
Hall, Nathan
Tian, Xingshan
Yu, Qin
Patterson, Eric L.
author_sort Zhang, Chun
collection PubMed
description Genomic structural variation (SV) has profound effects on organismal evolution; often serving as a source of novel genetic variation. Gene copy number variation (CNV), one type of SV, has repeatedly been associated with adaptive evolution in eukaryotes, especially with environmental stress. Resistance to the widely used herbicide, glyphosate, has evolved through target-site CNV in many weedy plant species, including the economically important grass, Eleusine indica (goosegrass); however, the origin and mechanism of these CNVs remain elusive in many weed species due to limited genetic and genomic resources. To study this CNV in goosegrass, we present high-quality reference genomes for glyphosate-susceptible and -resistant goosegrass lines and fine-assembles of the duplication of glyphosate’s target site gene 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). We reveal a unique rearrangement of EPSPS involving chromosome subtelomeres. This discovery adds to the limited knowledge of the importance of subtelomeres as genetic variation generators and provides another unique example for herbicide resistance evolution.
format Online
Article
Text
id pubmed-10421919
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-104219192023-08-13 Subtelomeric 5-enolpyruvylshikimate-3-phosphate synthase copy number variation confers glyphosate resistance in Eleusine indica Zhang, Chun Johnson, Nicholas A. Hall, Nathan Tian, Xingshan Yu, Qin Patterson, Eric L. Nat Commun Article Genomic structural variation (SV) has profound effects on organismal evolution; often serving as a source of novel genetic variation. Gene copy number variation (CNV), one type of SV, has repeatedly been associated with adaptive evolution in eukaryotes, especially with environmental stress. Resistance to the widely used herbicide, glyphosate, has evolved through target-site CNV in many weedy plant species, including the economically important grass, Eleusine indica (goosegrass); however, the origin and mechanism of these CNVs remain elusive in many weed species due to limited genetic and genomic resources. To study this CNV in goosegrass, we present high-quality reference genomes for glyphosate-susceptible and -resistant goosegrass lines and fine-assembles of the duplication of glyphosate’s target site gene 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). We reveal a unique rearrangement of EPSPS involving chromosome subtelomeres. This discovery adds to the limited knowledge of the importance of subtelomeres as genetic variation generators and provides another unique example for herbicide resistance evolution. Nature Publishing Group UK 2023-08-11 /pmc/articles/PMC10421919/ /pubmed/37567866 http://dx.doi.org/10.1038/s41467-023-40407-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Zhang, Chun
Johnson, Nicholas A.
Hall, Nathan
Tian, Xingshan
Yu, Qin
Patterson, Eric L.
Subtelomeric 5-enolpyruvylshikimate-3-phosphate synthase copy number variation confers glyphosate resistance in Eleusine indica
title Subtelomeric 5-enolpyruvylshikimate-3-phosphate synthase copy number variation confers glyphosate resistance in Eleusine indica
title_full Subtelomeric 5-enolpyruvylshikimate-3-phosphate synthase copy number variation confers glyphosate resistance in Eleusine indica
title_fullStr Subtelomeric 5-enolpyruvylshikimate-3-phosphate synthase copy number variation confers glyphosate resistance in Eleusine indica
title_full_unstemmed Subtelomeric 5-enolpyruvylshikimate-3-phosphate synthase copy number variation confers glyphosate resistance in Eleusine indica
title_short Subtelomeric 5-enolpyruvylshikimate-3-phosphate synthase copy number variation confers glyphosate resistance in Eleusine indica
title_sort subtelomeric 5-enolpyruvylshikimate-3-phosphate synthase copy number variation confers glyphosate resistance in eleusine indica
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421919/
https://www.ncbi.nlm.nih.gov/pubmed/37567866
http://dx.doi.org/10.1038/s41467-023-40407-6
work_keys_str_mv AT zhangchun subtelomeric5enolpyruvylshikimate3phosphatesynthasecopynumbervariationconfersglyphosateresistanceineleusineindica
AT johnsonnicholasa subtelomeric5enolpyruvylshikimate3phosphatesynthasecopynumbervariationconfersglyphosateresistanceineleusineindica
AT hallnathan subtelomeric5enolpyruvylshikimate3phosphatesynthasecopynumbervariationconfersglyphosateresistanceineleusineindica
AT tianxingshan subtelomeric5enolpyruvylshikimate3phosphatesynthasecopynumbervariationconfersglyphosateresistanceineleusineindica
AT yuqin subtelomeric5enolpyruvylshikimate3phosphatesynthasecopynumbervariationconfersglyphosateresistanceineleusineindica
AT pattersonericl subtelomeric5enolpyruvylshikimate3phosphatesynthasecopynumbervariationconfersglyphosateresistanceineleusineindica