Cargando…
In utero position matters for littermate cell transfer in mice: an additional and confounding source with maternal microchimerism
INTRODUCTION: Feto-maternal cell transfer during pregnancy is called microchimerism (Mc). Its persistence in respective hosts is increasingly studied as to its potential role in immune tolerance, autoimmunity, cancer, and degenerative diseases. Murine models with transgenic reporter genes, heterozyg...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10422045/ https://www.ncbi.nlm.nih.gov/pubmed/37575249 http://dx.doi.org/10.3389/fimmu.2023.1200920 |
_version_ | 1785089107866681344 |
---|---|
author | Giassi, Mathilde Hemon, Marie F. Martin, Marielle Roudier, Jean Auger, Isabelle Lambert, Nathalie C. |
author_facet | Giassi, Mathilde Hemon, Marie F. Martin, Marielle Roudier, Jean Auger, Isabelle Lambert, Nathalie C. |
author_sort | Giassi, Mathilde |
collection | PubMed |
description | INTRODUCTION: Feto-maternal cell transfer during pregnancy is called microchimerism (Mc). Its persistence in respective hosts is increasingly studied as to its potential role in immune tolerance, autoimmunity, cancer, and degenerative diseases. Murine models with transgenic reporter genes, heterozygously carried by the mother, allow maternal Mc tracking in wild-type (WT) offspring. However, as gestation in mice is multi-embryonic, an exchange of cells between fetuses carrying the same reporter gene as their mother and negative WT littermate, named littermate Mc (LMc), can occur and be confounded with the maternal source. We propose here to evaluate LMc contribution in mice. METHODS: To avoid the maternal confounding source of Mc, transgenic males, heterozygous for a reporter gene, here, the human leukocyte antigen DRB1*04 (DR4(+/−)), were crossed with WT females (DR4(−/−)). DR4(+/−) LMc was specifically quantified by HLA-DR4 quantitative PCR, i) in utero in main organs from 15 DR4(−/−) fetuses from three litters of 11, nine, and five; and ii) after birth in two litters of eight pups: in two DR4(−/−) stillborns and four DR4(−/−) adult mice. RESULTS: At embryonic stages, DR4(−/−) fetuses having one or two nearby DR4(+/−) littermates in the same uterine horn were almost seven times more frequently positive for DR4− microchimerism in their organs (p = 0.01) and had quantitatively more LMc (p = 0.009) than those without nearby DR4(+/−) littermates. Furthermore, LMc persists at birth and into adulthood with interindividual heterogeneity. CONCLUSIONS: This study identifies heterogeneity for LMc acquisition according to in utero position and different interpretation of previously published results on maternal Mc in mice. |
format | Online Article Text |
id | pubmed-10422045 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-104220452023-08-13 In utero position matters for littermate cell transfer in mice: an additional and confounding source with maternal microchimerism Giassi, Mathilde Hemon, Marie F. Martin, Marielle Roudier, Jean Auger, Isabelle Lambert, Nathalie C. Front Immunol Immunology INTRODUCTION: Feto-maternal cell transfer during pregnancy is called microchimerism (Mc). Its persistence in respective hosts is increasingly studied as to its potential role in immune tolerance, autoimmunity, cancer, and degenerative diseases. Murine models with transgenic reporter genes, heterozygously carried by the mother, allow maternal Mc tracking in wild-type (WT) offspring. However, as gestation in mice is multi-embryonic, an exchange of cells between fetuses carrying the same reporter gene as their mother and negative WT littermate, named littermate Mc (LMc), can occur and be confounded with the maternal source. We propose here to evaluate LMc contribution in mice. METHODS: To avoid the maternal confounding source of Mc, transgenic males, heterozygous for a reporter gene, here, the human leukocyte antigen DRB1*04 (DR4(+/−)), were crossed with WT females (DR4(−/−)). DR4(+/−) LMc was specifically quantified by HLA-DR4 quantitative PCR, i) in utero in main organs from 15 DR4(−/−) fetuses from three litters of 11, nine, and five; and ii) after birth in two litters of eight pups: in two DR4(−/−) stillborns and four DR4(−/−) adult mice. RESULTS: At embryonic stages, DR4(−/−) fetuses having one or two nearby DR4(+/−) littermates in the same uterine horn were almost seven times more frequently positive for DR4− microchimerism in their organs (p = 0.01) and had quantitatively more LMc (p = 0.009) than those without nearby DR4(+/−) littermates. Furthermore, LMc persists at birth and into adulthood with interindividual heterogeneity. CONCLUSIONS: This study identifies heterogeneity for LMc acquisition according to in utero position and different interpretation of previously published results on maternal Mc in mice. Frontiers Media S.A. 2023-07-28 /pmc/articles/PMC10422045/ /pubmed/37575249 http://dx.doi.org/10.3389/fimmu.2023.1200920 Text en Copyright © 2023 Giassi, Hemon, Martin, Roudier, Auger and Lambert https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Giassi, Mathilde Hemon, Marie F. Martin, Marielle Roudier, Jean Auger, Isabelle Lambert, Nathalie C. In utero position matters for littermate cell transfer in mice: an additional and confounding source with maternal microchimerism |
title |
In utero position matters for littermate cell transfer in mice: an additional and confounding source with maternal microchimerism |
title_full |
In utero position matters for littermate cell transfer in mice: an additional and confounding source with maternal microchimerism |
title_fullStr |
In utero position matters for littermate cell transfer in mice: an additional and confounding source with maternal microchimerism |
title_full_unstemmed |
In utero position matters for littermate cell transfer in mice: an additional and confounding source with maternal microchimerism |
title_short |
In utero position matters for littermate cell transfer in mice: an additional and confounding source with maternal microchimerism |
title_sort | in utero position matters for littermate cell transfer in mice: an additional and confounding source with maternal microchimerism |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10422045/ https://www.ncbi.nlm.nih.gov/pubmed/37575249 http://dx.doi.org/10.3389/fimmu.2023.1200920 |
work_keys_str_mv | AT giassimathilde inuteropositionmattersforlittermatecelltransferinmiceanadditionalandconfoundingsourcewithmaternalmicrochimerism AT hemonmarief inuteropositionmattersforlittermatecelltransferinmiceanadditionalandconfoundingsourcewithmaternalmicrochimerism AT martinmarielle inuteropositionmattersforlittermatecelltransferinmiceanadditionalandconfoundingsourcewithmaternalmicrochimerism AT roudierjean inuteropositionmattersforlittermatecelltransferinmiceanadditionalandconfoundingsourcewithmaternalmicrochimerism AT augerisabelle inuteropositionmattersforlittermatecelltransferinmiceanadditionalandconfoundingsourcewithmaternalmicrochimerism AT lambertnathaliec inuteropositionmattersforlittermatecelltransferinmiceanadditionalandconfoundingsourcewithmaternalmicrochimerism |