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The impact of shift-work light conditions on tissue-specific circadian rhythms of canonical clock genes: insights from a mouse model study
Background: The natural day-night cycle synchronizes our circadian rhythms, but modern work practices like night shifts disrupt this pattern, leading to increased exposure to nighttime light. This exposure is linked to various health issues. While some studies have explored the effects of night shif...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
F1000 Research Limited
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10422053/ https://www.ncbi.nlm.nih.gov/pubmed/37576540 http://dx.doi.org/10.12688/f1000research.136998.3 |
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author | Koritala, Bala S. C. Dakup, Panshak P. Porter, Kenneth I. Gaddameedhi, Shobhan |
author_facet | Koritala, Bala S. C. Dakup, Panshak P. Porter, Kenneth I. Gaddameedhi, Shobhan |
author_sort | Koritala, Bala S. C. |
collection | PubMed |
description | Background: The natural day-night cycle synchronizes our circadian rhythms, but modern work practices like night shifts disrupt this pattern, leading to increased exposure to nighttime light. This exposure is linked to various health issues. While some studies have explored the effects of night shifts on human circadian rhythms, there is limited research on the consequences of long-term exposure to shift-work light conditions. Rodents can provide valuable insights into these effects. This study aimed to examine how short- or long-term exposure to rotating shifts and chronic jetlag affects the core circadian oscillators in the liver and skin of mammals. Methods: C57BL/6J male mice were subjected to simulated shift-work light conditions, including short-term or long-term rotating shifts and chronic jet-lag conditions. Liver and skin samples were collected every four hours over a 24-hour period on the second day of constant darkness. RNA was extracted and qRT-PCR analysis was conducted to measure the circadian gene expression in liver and skin tissues. Circadian rhythm analysis using CircaCompare compared the control group to mice exposed to shift-work light conditions. Results: The liver's circadian clock is significantly altered in mice under long-term rotating shift conditions, with a lesser but still noticeable impact in mice experiencing chronic jetlag. However, short-term rotating shift conditions do not significantly affect the liver's circadian clock. Conversely, all three simulated shift conditions affect the skin's circadian clock, indicating that the skin clock is more sensitive to shift-work light conditions than the liver clock. Compared to the liver, the skin's circadian clock is greatly affected by long-term rotating shift conditions. Conclusions: The study findings indicate more pronounced disturbances in the canonical clock genes of the skin compared to the liver under simulated shift-work light conditions. These results suggest that the skin clock is more vulnerable to the effects of shift-work. |
format | Online Article Text |
id | pubmed-10422053 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | F1000 Research Limited |
record_format | MEDLINE/PubMed |
spelling | pubmed-104220532023-08-13 The impact of shift-work light conditions on tissue-specific circadian rhythms of canonical clock genes: insights from a mouse model study Koritala, Bala S. C. Dakup, Panshak P. Porter, Kenneth I. Gaddameedhi, Shobhan F1000Res Research Article Background: The natural day-night cycle synchronizes our circadian rhythms, but modern work practices like night shifts disrupt this pattern, leading to increased exposure to nighttime light. This exposure is linked to various health issues. While some studies have explored the effects of night shifts on human circadian rhythms, there is limited research on the consequences of long-term exposure to shift-work light conditions. Rodents can provide valuable insights into these effects. This study aimed to examine how short- or long-term exposure to rotating shifts and chronic jetlag affects the core circadian oscillators in the liver and skin of mammals. Methods: C57BL/6J male mice were subjected to simulated shift-work light conditions, including short-term or long-term rotating shifts and chronic jet-lag conditions. Liver and skin samples were collected every four hours over a 24-hour period on the second day of constant darkness. RNA was extracted and qRT-PCR analysis was conducted to measure the circadian gene expression in liver and skin tissues. Circadian rhythm analysis using CircaCompare compared the control group to mice exposed to shift-work light conditions. Results: The liver's circadian clock is significantly altered in mice under long-term rotating shift conditions, with a lesser but still noticeable impact in mice experiencing chronic jetlag. However, short-term rotating shift conditions do not significantly affect the liver's circadian clock. Conversely, all three simulated shift conditions affect the skin's circadian clock, indicating that the skin clock is more sensitive to shift-work light conditions than the liver clock. Compared to the liver, the skin's circadian clock is greatly affected by long-term rotating shift conditions. Conclusions: The study findings indicate more pronounced disturbances in the canonical clock genes of the skin compared to the liver under simulated shift-work light conditions. These results suggest that the skin clock is more vulnerable to the effects of shift-work. F1000 Research Limited 2023-08-17 /pmc/articles/PMC10422053/ /pubmed/37576540 http://dx.doi.org/10.12688/f1000research.136998.3 Text en Copyright: © 2023 Koritala BSC et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Koritala, Bala S. C. Dakup, Panshak P. Porter, Kenneth I. Gaddameedhi, Shobhan The impact of shift-work light conditions on tissue-specific circadian rhythms of canonical clock genes: insights from a mouse model study |
title | The impact of shift-work light conditions on tissue-specific circadian rhythms of canonical clock genes: insights from a mouse model study |
title_full | The impact of shift-work light conditions on tissue-specific circadian rhythms of canonical clock genes: insights from a mouse model study |
title_fullStr | The impact of shift-work light conditions on tissue-specific circadian rhythms of canonical clock genes: insights from a mouse model study |
title_full_unstemmed | The impact of shift-work light conditions on tissue-specific circadian rhythms of canonical clock genes: insights from a mouse model study |
title_short | The impact of shift-work light conditions on tissue-specific circadian rhythms of canonical clock genes: insights from a mouse model study |
title_sort | impact of shift-work light conditions on tissue-specific circadian rhythms of canonical clock genes: insights from a mouse model study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10422053/ https://www.ncbi.nlm.nih.gov/pubmed/37576540 http://dx.doi.org/10.12688/f1000research.136998.3 |
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