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WHIRLY proteins maintain seed longevity by effects on seed oxygen signalling during imbibition

The WHIRLY (WHY) family of DNA/RNA binding proteins fulfil multiple but poorly characterised functions in plants. We analysed WHY protein functions in the Arabidopsis Atwhy1, Atwhy3, Atwhy1why3 single and double mutants and wild type controls. The Atwhy3 and Atwhy1why3 double mutants showed a signif...

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Autores principales: Taylor, Rachel E., Waterworth, Wanda, West, Christopher E, Foyer, Christine H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10422932/
https://www.ncbi.nlm.nih.gov/pubmed/37351567
http://dx.doi.org/10.1042/BCJ20230008
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author Taylor, Rachel E.
Waterworth, Wanda
West, Christopher E
Foyer, Christine H.
author_facet Taylor, Rachel E.
Waterworth, Wanda
West, Christopher E
Foyer, Christine H.
author_sort Taylor, Rachel E.
collection PubMed
description The WHIRLY (WHY) family of DNA/RNA binding proteins fulfil multiple but poorly characterised functions in plants. We analysed WHY protein functions in the Arabidopsis Atwhy1, Atwhy3, Atwhy1why3 single and double mutants and wild type controls. The Atwhy3 and Atwhy1why3 double mutants showed a significant delay in flowering, having more siliques per plant but with fewer seeds per silique than the wild type. While germination was similar in the unaged high-quality seeds of all lines, significant decreases in vigour and viability were observed in the aged mutant seeds compared with the wild type. Imbibition of unaged high-quality seeds was characterised by large increases in transcripts that encode proteins involved in oxygen sensing and responses to hypoxia. Seed aging resulted in a disruption of the imbibition-induced transcriptome profile such that transcripts encoding RNA metabolism and processing became the most abundant components of the imbibition signature. The imbibition-related profile of the Atwhy1why3 mutant seeds, was characterised by decreased expression of hypoxia-related and oxygen metabolism genes even in the absence of aging. Seed aging further decreased the abundance of hypoxia-related and oxygen metabolism transcripts relative to the wild type. These findings suggest that the WHY1 and WHY3 proteins regulate the imbibition-induced responses to oxygen availability and hypoxia. Loss of WHY1 and WHY3 functions decreases the ability of Arabidopsis seeds to resist the adverse effects of seed aging.
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spelling pubmed-104229322023-08-13 WHIRLY proteins maintain seed longevity by effects on seed oxygen signalling during imbibition Taylor, Rachel E. Waterworth, Wanda West, Christopher E Foyer, Christine H. Biochem J Plant Biology The WHIRLY (WHY) family of DNA/RNA binding proteins fulfil multiple but poorly characterised functions in plants. We analysed WHY protein functions in the Arabidopsis Atwhy1, Atwhy3, Atwhy1why3 single and double mutants and wild type controls. The Atwhy3 and Atwhy1why3 double mutants showed a significant delay in flowering, having more siliques per plant but with fewer seeds per silique than the wild type. While germination was similar in the unaged high-quality seeds of all lines, significant decreases in vigour and viability were observed in the aged mutant seeds compared with the wild type. Imbibition of unaged high-quality seeds was characterised by large increases in transcripts that encode proteins involved in oxygen sensing and responses to hypoxia. Seed aging resulted in a disruption of the imbibition-induced transcriptome profile such that transcripts encoding RNA metabolism and processing became the most abundant components of the imbibition signature. The imbibition-related profile of the Atwhy1why3 mutant seeds, was characterised by decreased expression of hypoxia-related and oxygen metabolism genes even in the absence of aging. Seed aging further decreased the abundance of hypoxia-related and oxygen metabolism transcripts relative to the wild type. These findings suggest that the WHY1 and WHY3 proteins regulate the imbibition-induced responses to oxygen availability and hypoxia. Loss of WHY1 and WHY3 functions decreases the ability of Arabidopsis seeds to resist the adverse effects of seed aging. Portland Press Ltd. 2023-07-06 /pmc/articles/PMC10422932/ /pubmed/37351567 http://dx.doi.org/10.1042/BCJ20230008 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . Open access for this article was enabled by the participation of University of Birmingham in an all-inclusive Read & Publish agreement with Portland Press and the Biochemical Society under a transformative agreement with JISC.
spellingShingle Plant Biology
Taylor, Rachel E.
Waterworth, Wanda
West, Christopher E
Foyer, Christine H.
WHIRLY proteins maintain seed longevity by effects on seed oxygen signalling during imbibition
title WHIRLY proteins maintain seed longevity by effects on seed oxygen signalling during imbibition
title_full WHIRLY proteins maintain seed longevity by effects on seed oxygen signalling during imbibition
title_fullStr WHIRLY proteins maintain seed longevity by effects on seed oxygen signalling during imbibition
title_full_unstemmed WHIRLY proteins maintain seed longevity by effects on seed oxygen signalling during imbibition
title_short WHIRLY proteins maintain seed longevity by effects on seed oxygen signalling during imbibition
title_sort whirly proteins maintain seed longevity by effects on seed oxygen signalling during imbibition
topic Plant Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10422932/
https://www.ncbi.nlm.nih.gov/pubmed/37351567
http://dx.doi.org/10.1042/BCJ20230008
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