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Rootstock‐induced scion resistance against tobacco mosaic virus is associated with the induction of defence‐related transcripts and graft‐transmissible mRNAs

Grafting is a common horticultural practice used to confer desirable traits between rootstock and scion, including disease resistance. To investigate graft‐conferred resistance against viral diseases a novel heterografting system was developed using Nicotiana benthamiana scions grafted onto differen...

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Autores principales: Kappagantu, Madhu, Brandon, Matthew, Tamukong, Yvette B., Culver, James N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10423323/
https://www.ncbi.nlm.nih.gov/pubmed/37191642
http://dx.doi.org/10.1111/mpp.13353
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author Kappagantu, Madhu
Brandon, Matthew
Tamukong, Yvette B.
Culver, James N.
author_facet Kappagantu, Madhu
Brandon, Matthew
Tamukong, Yvette B.
Culver, James N.
author_sort Kappagantu, Madhu
collection PubMed
description Grafting is a common horticultural practice used to confer desirable traits between rootstock and scion, including disease resistance. To investigate graft‐conferred resistance against viral diseases a novel heterografting system was developed using Nicotiana benthamiana scions grafted onto different tomato rootstocks. N. benthamiana is normally highly susceptible to tobacco mosaic virus (TMV) infection. However, specific tomato rootstock varieties were found to confer a range of resistance levels to N. benthamiana scions inoculated with TMV. Conferred resistance was associated with delays in virus accumulation and the reduction in virus spread. RNA sequencing analysis showed the enrichment of transcripts associated with disease resistance and plant stress in N. benthamiana scions grafted onto resistance‐inducing tomato rootstocks. Genome sequencing of resistance‐ and nonresistance‐conferring rootstocks was used to identify mobile tomato transcripts within N. benthamiana scions. Within resistance‐induced N. benthamiana scions, enriched mobile tomato transcripts were predominantly associated with defence, stress, and abscisic acid signalling when compared to similar scions grafted onto nonresistance‐inducing rootstock. Combining these findings suggests that graft‐induced resistance is modulated by rootstock scion transcriptional responses and rootstock‐specific mobile transcripts.
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spelling pubmed-104233232023-08-14 Rootstock‐induced scion resistance against tobacco mosaic virus is associated with the induction of defence‐related transcripts and graft‐transmissible mRNAs Kappagantu, Madhu Brandon, Matthew Tamukong, Yvette B. Culver, James N. Mol Plant Pathol Short Communication Grafting is a common horticultural practice used to confer desirable traits between rootstock and scion, including disease resistance. To investigate graft‐conferred resistance against viral diseases a novel heterografting system was developed using Nicotiana benthamiana scions grafted onto different tomato rootstocks. N. benthamiana is normally highly susceptible to tobacco mosaic virus (TMV) infection. However, specific tomato rootstock varieties were found to confer a range of resistance levels to N. benthamiana scions inoculated with TMV. Conferred resistance was associated with delays in virus accumulation and the reduction in virus spread. RNA sequencing analysis showed the enrichment of transcripts associated with disease resistance and plant stress in N. benthamiana scions grafted onto resistance‐inducing tomato rootstocks. Genome sequencing of resistance‐ and nonresistance‐conferring rootstocks was used to identify mobile tomato transcripts within N. benthamiana scions. Within resistance‐induced N. benthamiana scions, enriched mobile tomato transcripts were predominantly associated with defence, stress, and abscisic acid signalling when compared to similar scions grafted onto nonresistance‐inducing rootstock. Combining these findings suggests that graft‐induced resistance is modulated by rootstock scion transcriptional responses and rootstock‐specific mobile transcripts. John Wiley and Sons Inc. 2023-05-16 /pmc/articles/PMC10423323/ /pubmed/37191642 http://dx.doi.org/10.1111/mpp.13353 Text en © 2023 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Short Communication
Kappagantu, Madhu
Brandon, Matthew
Tamukong, Yvette B.
Culver, James N.
Rootstock‐induced scion resistance against tobacco mosaic virus is associated with the induction of defence‐related transcripts and graft‐transmissible mRNAs
title Rootstock‐induced scion resistance against tobacco mosaic virus is associated with the induction of defence‐related transcripts and graft‐transmissible mRNAs
title_full Rootstock‐induced scion resistance against tobacco mosaic virus is associated with the induction of defence‐related transcripts and graft‐transmissible mRNAs
title_fullStr Rootstock‐induced scion resistance against tobacco mosaic virus is associated with the induction of defence‐related transcripts and graft‐transmissible mRNAs
title_full_unstemmed Rootstock‐induced scion resistance against tobacco mosaic virus is associated with the induction of defence‐related transcripts and graft‐transmissible mRNAs
title_short Rootstock‐induced scion resistance against tobacco mosaic virus is associated with the induction of defence‐related transcripts and graft‐transmissible mRNAs
title_sort rootstock‐induced scion resistance against tobacco mosaic virus is associated with the induction of defence‐related transcripts and graft‐transmissible mrnas
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10423323/
https://www.ncbi.nlm.nih.gov/pubmed/37191642
http://dx.doi.org/10.1111/mpp.13353
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