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A nonclassically secreted effector of Magnaporthe oryzae targets host nuclei and plays important roles in fungal growth and plant infection

Rice blast caused by Magnaporthe oryzae is one of the most destructive diseases and poses a growing threat to food security worldwide. Like many other filamentous pathogens, rice blast fungus releases multiple types of effector proteins to facilitate fungal infection and modulate host defence respon...

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Autores principales: Chen, Xiaomin, Pan, Su, Bai, Huimin, Fan, Jiaxin, Batool, Wajjiha, Shabbir, Ammarah, Han, Yijuan, Zheng, Huakun, Lu, Guodong, Lin, Lili, Tang, Wei, Wang, Zonghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10423324/
https://www.ncbi.nlm.nih.gov/pubmed/37306516
http://dx.doi.org/10.1111/mpp.13356
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author Chen, Xiaomin
Pan, Su
Bai, Huimin
Fan, Jiaxin
Batool, Wajjiha
Shabbir, Ammarah
Han, Yijuan
Zheng, Huakun
Lu, Guodong
Lin, Lili
Tang, Wei
Wang, Zonghua
author_facet Chen, Xiaomin
Pan, Su
Bai, Huimin
Fan, Jiaxin
Batool, Wajjiha
Shabbir, Ammarah
Han, Yijuan
Zheng, Huakun
Lu, Guodong
Lin, Lili
Tang, Wei
Wang, Zonghua
author_sort Chen, Xiaomin
collection PubMed
description Rice blast caused by Magnaporthe oryzae is one of the most destructive diseases and poses a growing threat to food security worldwide. Like many other filamentous pathogens, rice blast fungus releases multiple types of effector proteins to facilitate fungal infection and modulate host defence responses. However, most of the characterized effectors contain an N‐terminal signal peptide. Here, we report the results of the functional characterization of a nonclassically secreted nuclear targeting effector in M. oryzae (MoNte1). MoNte1 has no signal peptide, but can be secreted and translocated into plant nuclei driven by a nuclear targeting peptide. It could also induce hypersensitive cell death when transiently expressed in Nicotiana benthamiana. Deletion of the MoNTE1 gene caused a significant reduction of fungal growth and conidiogenesis, partially impaired appressorium formation and host colonization, and also dramatically attenuated the pathogenicity. Taken together, these findings reveal a novel effector secretion pathway and deepen our understanding of rice–M. oryzae interactions.
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spelling pubmed-104233242023-08-14 A nonclassically secreted effector of Magnaporthe oryzae targets host nuclei and plays important roles in fungal growth and plant infection Chen, Xiaomin Pan, Su Bai, Huimin Fan, Jiaxin Batool, Wajjiha Shabbir, Ammarah Han, Yijuan Zheng, Huakun Lu, Guodong Lin, Lili Tang, Wei Wang, Zonghua Mol Plant Pathol Original Articles Rice blast caused by Magnaporthe oryzae is one of the most destructive diseases and poses a growing threat to food security worldwide. Like many other filamentous pathogens, rice blast fungus releases multiple types of effector proteins to facilitate fungal infection and modulate host defence responses. However, most of the characterized effectors contain an N‐terminal signal peptide. Here, we report the results of the functional characterization of a nonclassically secreted nuclear targeting effector in M. oryzae (MoNte1). MoNte1 has no signal peptide, but can be secreted and translocated into plant nuclei driven by a nuclear targeting peptide. It could also induce hypersensitive cell death when transiently expressed in Nicotiana benthamiana. Deletion of the MoNTE1 gene caused a significant reduction of fungal growth and conidiogenesis, partially impaired appressorium formation and host colonization, and also dramatically attenuated the pathogenicity. Taken together, these findings reveal a novel effector secretion pathway and deepen our understanding of rice–M. oryzae interactions. John Wiley and Sons Inc. 2023-06-12 /pmc/articles/PMC10423324/ /pubmed/37306516 http://dx.doi.org/10.1111/mpp.13356 Text en © 2023 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Chen, Xiaomin
Pan, Su
Bai, Huimin
Fan, Jiaxin
Batool, Wajjiha
Shabbir, Ammarah
Han, Yijuan
Zheng, Huakun
Lu, Guodong
Lin, Lili
Tang, Wei
Wang, Zonghua
A nonclassically secreted effector of Magnaporthe oryzae targets host nuclei and plays important roles in fungal growth and plant infection
title A nonclassically secreted effector of Magnaporthe oryzae targets host nuclei and plays important roles in fungal growth and plant infection
title_full A nonclassically secreted effector of Magnaporthe oryzae targets host nuclei and plays important roles in fungal growth and plant infection
title_fullStr A nonclassically secreted effector of Magnaporthe oryzae targets host nuclei and plays important roles in fungal growth and plant infection
title_full_unstemmed A nonclassically secreted effector of Magnaporthe oryzae targets host nuclei and plays important roles in fungal growth and plant infection
title_short A nonclassically secreted effector of Magnaporthe oryzae targets host nuclei and plays important roles in fungal growth and plant infection
title_sort nonclassically secreted effector of magnaporthe oryzae targets host nuclei and plays important roles in fungal growth and plant infection
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10423324/
https://www.ncbi.nlm.nih.gov/pubmed/37306516
http://dx.doi.org/10.1111/mpp.13356
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