Cargando…
Enhancement of β-carotene content in Chlamydomonas reinhardtii by expressing bacterium-driven lycopene β-cyclase
β-Carotene is one of the economically important carotenoids, having functions as the antioxidant to remove harmful free radicals and as the precursor for vitamin A and other high-valued xanthophyll such as zeaxanthin and astaxanthin. Lycopene cyclase plays an important role in the branching of β-car...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10423417/ https://www.ncbi.nlm.nih.gov/pubmed/37573357 http://dx.doi.org/10.1186/s13068-023-02377-1 |
_version_ | 1785089445509201920 |
---|---|
author | Huang, Danqiong Liu, Chenglong Su, Mingshan Zeng, Zhiyong Wang, Chaogang Hu, Zhangli Lou, Sulin Li, Hui |
author_facet | Huang, Danqiong Liu, Chenglong Su, Mingshan Zeng, Zhiyong Wang, Chaogang Hu, Zhangli Lou, Sulin Li, Hui |
author_sort | Huang, Danqiong |
collection | PubMed |
description | β-Carotene is one of the economically important carotenoids, having functions as the antioxidant to remove harmful free radicals and as the precursor for vitamin A and other high-valued xanthophyll such as zeaxanthin and astaxanthin. Lycopene cyclase plays an important role in the branching of β-carotene and α-carotene. Aiming to develop the microalgae with enhanced β-carotene productivity, the CrtY gene from bacterium Pantoea agglomerans was integrated into Chlamydomonas reinhardtii. The lycopene-producing E. coli harboring CrtY gene produced 1.59 times of β-carotene than that harboring DsLcyb1 from Dunaliella salina (a microalga with abundant β-carotene), confirming the superior activity of CrtY on β-carotene biosynthesis. According to the pigment analysis by HPLC, in microalgal transformants that were confirmed by molecular analysis, the expression of CrtY significantly increased β-carotene content from 12.48 mg/g to 30.65 mg/g (dry weight), which is about 2.45-fold changes. It is noted that three out of five transformants have statistically significant higher amount of lutein, even though the increment was 20% in maximum. Besides, no growth defect was observed in the transformants. This is the first report of functional expression of prokaryotic gene in eukaryotic microalgae, which will widen the gene pool targeting carotenoids biosynthesis using microalgae as the factory and thereby provide more opportunity for high-valued products engineering in microalgae. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13068-023-02377-1. |
format | Online Article Text |
id | pubmed-10423417 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-104234172023-08-14 Enhancement of β-carotene content in Chlamydomonas reinhardtii by expressing bacterium-driven lycopene β-cyclase Huang, Danqiong Liu, Chenglong Su, Mingshan Zeng, Zhiyong Wang, Chaogang Hu, Zhangli Lou, Sulin Li, Hui Biotechnol Biofuels Bioprod Research β-Carotene is one of the economically important carotenoids, having functions as the antioxidant to remove harmful free radicals and as the precursor for vitamin A and other high-valued xanthophyll such as zeaxanthin and astaxanthin. Lycopene cyclase plays an important role in the branching of β-carotene and α-carotene. Aiming to develop the microalgae with enhanced β-carotene productivity, the CrtY gene from bacterium Pantoea agglomerans was integrated into Chlamydomonas reinhardtii. The lycopene-producing E. coli harboring CrtY gene produced 1.59 times of β-carotene than that harboring DsLcyb1 from Dunaliella salina (a microalga with abundant β-carotene), confirming the superior activity of CrtY on β-carotene biosynthesis. According to the pigment analysis by HPLC, in microalgal transformants that were confirmed by molecular analysis, the expression of CrtY significantly increased β-carotene content from 12.48 mg/g to 30.65 mg/g (dry weight), which is about 2.45-fold changes. It is noted that three out of five transformants have statistically significant higher amount of lutein, even though the increment was 20% in maximum. Besides, no growth defect was observed in the transformants. This is the first report of functional expression of prokaryotic gene in eukaryotic microalgae, which will widen the gene pool targeting carotenoids biosynthesis using microalgae as the factory and thereby provide more opportunity for high-valued products engineering in microalgae. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13068-023-02377-1. BioMed Central 2023-08-12 /pmc/articles/PMC10423417/ /pubmed/37573357 http://dx.doi.org/10.1186/s13068-023-02377-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Huang, Danqiong Liu, Chenglong Su, Mingshan Zeng, Zhiyong Wang, Chaogang Hu, Zhangli Lou, Sulin Li, Hui Enhancement of β-carotene content in Chlamydomonas reinhardtii by expressing bacterium-driven lycopene β-cyclase |
title | Enhancement of β-carotene content in Chlamydomonas reinhardtii by expressing bacterium-driven lycopene β-cyclase |
title_full | Enhancement of β-carotene content in Chlamydomonas reinhardtii by expressing bacterium-driven lycopene β-cyclase |
title_fullStr | Enhancement of β-carotene content in Chlamydomonas reinhardtii by expressing bacterium-driven lycopene β-cyclase |
title_full_unstemmed | Enhancement of β-carotene content in Chlamydomonas reinhardtii by expressing bacterium-driven lycopene β-cyclase |
title_short | Enhancement of β-carotene content in Chlamydomonas reinhardtii by expressing bacterium-driven lycopene β-cyclase |
title_sort | enhancement of β-carotene content in chlamydomonas reinhardtii by expressing bacterium-driven lycopene β-cyclase |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10423417/ https://www.ncbi.nlm.nih.gov/pubmed/37573357 http://dx.doi.org/10.1186/s13068-023-02377-1 |
work_keys_str_mv | AT huangdanqiong enhancementofbcarotenecontentinchlamydomonasreinhardtiibyexpressingbacteriumdrivenlycopenebcyclase AT liuchenglong enhancementofbcarotenecontentinchlamydomonasreinhardtiibyexpressingbacteriumdrivenlycopenebcyclase AT sumingshan enhancementofbcarotenecontentinchlamydomonasreinhardtiibyexpressingbacteriumdrivenlycopenebcyclase AT zengzhiyong enhancementofbcarotenecontentinchlamydomonasreinhardtiibyexpressingbacteriumdrivenlycopenebcyclase AT wangchaogang enhancementofbcarotenecontentinchlamydomonasreinhardtiibyexpressingbacteriumdrivenlycopenebcyclase AT huzhangli enhancementofbcarotenecontentinchlamydomonasreinhardtiibyexpressingbacteriumdrivenlycopenebcyclase AT lousulin enhancementofbcarotenecontentinchlamydomonasreinhardtiibyexpressingbacteriumdrivenlycopenebcyclase AT lihui enhancementofbcarotenecontentinchlamydomonasreinhardtiibyexpressingbacteriumdrivenlycopenebcyclase |