Reducing Bias and Quantifying Uncertainty in Fluorescence Produced by PCR

We present a new approach for relating nucleic-acid content to fluorescence in a real-time Polymerase Chain Reaction (PCR) assay. By coupling a two-type branching process for PCR with a fluorescence analog of Beer’s Law, the approach reduces bias and quantifies uncertainty in fluorescence. As the tw...

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Detalles Bibliográficos
Autores principales: DeJaco, Robert F., Roberts, Matthew J., Romsos, Erica L., Vallone, Peter M., Kearsley, Anthony J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2023
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10423706/
https://www.ncbi.nlm.nih.gov/pubmed/37574503
http://dx.doi.org/10.1007/s11538-023-01182-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10423706/
https://www.ncbi.nlm.nih.gov/pubmed/37574503
http://dx.doi.org/10.1007/s11538-023-01182-z

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