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Reducing Bias and Quantifying Uncertainty in Fluorescence Produced by PCR
We present a new approach for relating nucleic-acid content to fluorescence in a real-time Polymerase Chain Reaction (PCR) assay. By coupling a two-type branching process for PCR with a fluorescence analog of Beer’s Law, the approach reduces bias and quantifies uncertainty in fluorescence. As the tw...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer US
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10423706/ https://www.ncbi.nlm.nih.gov/pubmed/37574503 http://dx.doi.org/10.1007/s11538-023-01182-z |