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Single opsin driven white noise ERGs in mice

PURPOSE: Electroretinograms elicited by photopigment isolating white noise stimuli (wnERGs) in mice were measured. The dependency of rod- and cone-opsin-driven wnERGs on mean luminance was studied. METHODS: Temporal white noise stimuli (containing all frequencies up to 20 Hz, equal amplitudes, rando...

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Autores principales: Stallwitz, Nina, Joachimsthaler, Anneka, Kremers, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10423813/
https://www.ncbi.nlm.nih.gov/pubmed/37583414
http://dx.doi.org/10.3389/fnins.2023.1211329
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author Stallwitz, Nina
Joachimsthaler, Anneka
Kremers, Jan
author_facet Stallwitz, Nina
Joachimsthaler, Anneka
Kremers, Jan
author_sort Stallwitz, Nina
collection PubMed
description PURPOSE: Electroretinograms elicited by photopigment isolating white noise stimuli (wnERGs) in mice were measured. The dependency of rod- and cone-opsin-driven wnERGs on mean luminance was studied. METHODS: Temporal white noise stimuli (containing all frequencies up to 20 Hz, equal amplitudes, random phases) that modulated either rhodopsin, S-opsin or L*-opsin, using the double silent substitution technique, were used to record wnERGs in mice expressing a human L*-opsin instead of the native murine M-opsin. Responses were recorded at 4 mean luminances (MLs). Impulse response functions (IRFs) were obtained by cross-correlating the wnERG recordings with the corresponding modulation of the photopigment excitation elicited by the stimulus. So-called modulation transfer functions (MTFs) were obtained by performing a Fourier transform on the IRFs. Potentials of two repeated wnERG recordings at corresponding time points were plotted against each other. The correlation coefficient (r(2)(repr)) of the linear regression through these data was used to quantify reproducibility. Another correlation coefficient (r(2)(ML)) was used to quantify the correlations of the wnERGs obtained at different MLs with those at the highest (for cone isolating stimuli) or lowest (for rod isolating stimuli) ML. RESULTS: IRFs showed an initial negative (a-wave like) trough N1 and a subsequent positive (b-wave like) peak P1. No oscillatory potential-like components were observed. At 0.4 and 1.0 log cd/m(2) ML robust L*- and S-opsin-driven IRFs were obtained that displayed similar latencies and dependencies on ML. L*-opsin-driven IRFs were 2.5–3 times larger than S-opsin-driven IRFs. Rhodopsin-driven IRFs were observed at −0.8 and − 0.2 log cd/m(2) and decreased in amplitude with increasing ML. They displayed an additional pronounced late negativity (N2), which may be a correlate of retinal ganglion cell activity. R(2)(repr) and r(2)(ML) values increased for cones with increasing ML whereas they decreased for rods. For rhodopsin-driven MTFs at low MLs and L*-opsin-driven MTFs at high MLs amplitudes decreased with increasing frequency, with much faster decreasing amplitudes for rhodopsin. A delay was calculated from MTF phases showing larger delays for rhodopsin- vs. low delays for L*-opsin-driven responses. CONCLUSION: Opsin-isolating wnERGs in mice show characteristics of different retinal cell types and their connected pathways.
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spelling pubmed-104238132023-08-15 Single opsin driven white noise ERGs in mice Stallwitz, Nina Joachimsthaler, Anneka Kremers, Jan Front Neurosci Neuroscience PURPOSE: Electroretinograms elicited by photopigment isolating white noise stimuli (wnERGs) in mice were measured. The dependency of rod- and cone-opsin-driven wnERGs on mean luminance was studied. METHODS: Temporal white noise stimuli (containing all frequencies up to 20 Hz, equal amplitudes, random phases) that modulated either rhodopsin, S-opsin or L*-opsin, using the double silent substitution technique, were used to record wnERGs in mice expressing a human L*-opsin instead of the native murine M-opsin. Responses were recorded at 4 mean luminances (MLs). Impulse response functions (IRFs) were obtained by cross-correlating the wnERG recordings with the corresponding modulation of the photopigment excitation elicited by the stimulus. So-called modulation transfer functions (MTFs) were obtained by performing a Fourier transform on the IRFs. Potentials of two repeated wnERG recordings at corresponding time points were plotted against each other. The correlation coefficient (r(2)(repr)) of the linear regression through these data was used to quantify reproducibility. Another correlation coefficient (r(2)(ML)) was used to quantify the correlations of the wnERGs obtained at different MLs with those at the highest (for cone isolating stimuli) or lowest (for rod isolating stimuli) ML. RESULTS: IRFs showed an initial negative (a-wave like) trough N1 and a subsequent positive (b-wave like) peak P1. No oscillatory potential-like components were observed. At 0.4 and 1.0 log cd/m(2) ML robust L*- and S-opsin-driven IRFs were obtained that displayed similar latencies and dependencies on ML. L*-opsin-driven IRFs were 2.5–3 times larger than S-opsin-driven IRFs. Rhodopsin-driven IRFs were observed at −0.8 and − 0.2 log cd/m(2) and decreased in amplitude with increasing ML. They displayed an additional pronounced late negativity (N2), which may be a correlate of retinal ganglion cell activity. R(2)(repr) and r(2)(ML) values increased for cones with increasing ML whereas they decreased for rods. For rhodopsin-driven MTFs at low MLs and L*-opsin-driven MTFs at high MLs amplitudes decreased with increasing frequency, with much faster decreasing amplitudes for rhodopsin. A delay was calculated from MTF phases showing larger delays for rhodopsin- vs. low delays for L*-opsin-driven responses. CONCLUSION: Opsin-isolating wnERGs in mice show characteristics of different retinal cell types and their connected pathways. Frontiers Media S.A. 2023-07-31 /pmc/articles/PMC10423813/ /pubmed/37583414 http://dx.doi.org/10.3389/fnins.2023.1211329 Text en Copyright © 2023 Stallwitz, Joachimsthaler and Kremers. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Stallwitz, Nina
Joachimsthaler, Anneka
Kremers, Jan
Single opsin driven white noise ERGs in mice
title Single opsin driven white noise ERGs in mice
title_full Single opsin driven white noise ERGs in mice
title_fullStr Single opsin driven white noise ERGs in mice
title_full_unstemmed Single opsin driven white noise ERGs in mice
title_short Single opsin driven white noise ERGs in mice
title_sort single opsin driven white noise ergs in mice
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10423813/
https://www.ncbi.nlm.nih.gov/pubmed/37583414
http://dx.doi.org/10.3389/fnins.2023.1211329
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