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The Larval Stages of Echinostoma spp. in Freshwater Snails as the First and Second Intermediate Hosts in Gilan and Mazandaran Provinces, Northern Iran

BACKGROUND: Identification of the larval stages of Echinostoma spp. in freshwater snails is an essential guide to continue monitoring the possibility of their transmission and the potential of echinostomiasis in areas where trematodes are the primary agent of parasitic diseases. The aim of this stud...

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Autores principales: Aryaeipour, Mojgan, Fard, Ramin Mazaheri Nezhad, Rad, Mohammad Bagher Molai, Pirestani, Majid, Rouhani, Soheila, Daryani, Ahmad, Asadi, Tina, Sarvi, Shahabeddin, Rokni, Mohammad Bagher
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10423910/
https://www.ncbi.nlm.nih.gov/pubmed/37583635
http://dx.doi.org/10.18502/ijpa.v18i2.13184
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author Aryaeipour, Mojgan
Fard, Ramin Mazaheri Nezhad
Rad, Mohammad Bagher Molai
Pirestani, Majid
Rouhani, Soheila
Daryani, Ahmad
Asadi, Tina
Sarvi, Shahabeddin
Rokni, Mohammad Bagher
author_facet Aryaeipour, Mojgan
Fard, Ramin Mazaheri Nezhad
Rad, Mohammad Bagher Molai
Pirestani, Majid
Rouhani, Soheila
Daryani, Ahmad
Asadi, Tina
Sarvi, Shahabeddin
Rokni, Mohammad Bagher
author_sort Aryaeipour, Mojgan
collection PubMed
description BACKGROUND: Identification of the larval stages of Echinostoma spp. in freshwater snails is an essential guide to continue monitoring the possibility of their transmission and the potential of echinostomiasis in areas where trematodes are the primary agent of parasitic diseases. The aim of this study was investigate Echinostoma using morphological and molecular techniques. METHODS: The study was conducted in Gilan and Mazandaran Provinces, northern Iran, from April 2019 to October 2021. Overall, 5300 freshwater snails were randomly collected and were identified using external shell morphology. Meanwhile, snails infected with trematodes were studied via shedding and dissecting methods. Larvae stages of Echinostoma were identified and the genomic DNA of the samples was extracted. The PCR amplification of the ITSI gene was carried out for 17 isolates and products were sequenced. Seven sequences were deposited in GenBank. RESULTS: Totally, 3.5% of snails containing three species (Stagnicola sp., Radix sp. and Planorbis sp.) were infected with two types of cercaria, E. revolutum with 37 and Echinostoma sp. with 45 spines in the collar. Moreover, 35% of the snails were infected with Echinostoma spp. metacercaria. Phylogenetic analysis illustrated that isolates were included in two ITSI haplogroups. CONCLUSION: Results showed the potential hazard of a zoonotic parasite as Echinostoma in northern Iran. The potential of disease environmental relationship investigation and resource control optimization is necessary for effective disease prevention and health management.
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spelling pubmed-104239102023-08-15 The Larval Stages of Echinostoma spp. in Freshwater Snails as the First and Second Intermediate Hosts in Gilan and Mazandaran Provinces, Northern Iran Aryaeipour, Mojgan Fard, Ramin Mazaheri Nezhad Rad, Mohammad Bagher Molai Pirestani, Majid Rouhani, Soheila Daryani, Ahmad Asadi, Tina Sarvi, Shahabeddin Rokni, Mohammad Bagher Iran J Parasitol Original Article BACKGROUND: Identification of the larval stages of Echinostoma spp. in freshwater snails is an essential guide to continue monitoring the possibility of their transmission and the potential of echinostomiasis in areas where trematodes are the primary agent of parasitic diseases. The aim of this study was investigate Echinostoma using morphological and molecular techniques. METHODS: The study was conducted in Gilan and Mazandaran Provinces, northern Iran, from April 2019 to October 2021. Overall, 5300 freshwater snails were randomly collected and were identified using external shell morphology. Meanwhile, snails infected with trematodes were studied via shedding and dissecting methods. Larvae stages of Echinostoma were identified and the genomic DNA of the samples was extracted. The PCR amplification of the ITSI gene was carried out for 17 isolates and products were sequenced. Seven sequences were deposited in GenBank. RESULTS: Totally, 3.5% of snails containing three species (Stagnicola sp., Radix sp. and Planorbis sp.) were infected with two types of cercaria, E. revolutum with 37 and Echinostoma sp. with 45 spines in the collar. Moreover, 35% of the snails were infected with Echinostoma spp. metacercaria. Phylogenetic analysis illustrated that isolates were included in two ITSI haplogroups. CONCLUSION: Results showed the potential hazard of a zoonotic parasite as Echinostoma in northern Iran. The potential of disease environmental relationship investigation and resource control optimization is necessary for effective disease prevention and health management. Tehran University of Medical Sciences 2023 /pmc/articles/PMC10423910/ /pubmed/37583635 http://dx.doi.org/10.18502/ijpa.v18i2.13184 Text en Copyright © 2023 Aryaeipour et al. Published by Tehran University of Medical Sciences. https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International license (https://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited.
spellingShingle Original Article
Aryaeipour, Mojgan
Fard, Ramin Mazaheri Nezhad
Rad, Mohammad Bagher Molai
Pirestani, Majid
Rouhani, Soheila
Daryani, Ahmad
Asadi, Tina
Sarvi, Shahabeddin
Rokni, Mohammad Bagher
The Larval Stages of Echinostoma spp. in Freshwater Snails as the First and Second Intermediate Hosts in Gilan and Mazandaran Provinces, Northern Iran
title The Larval Stages of Echinostoma spp. in Freshwater Snails as the First and Second Intermediate Hosts in Gilan and Mazandaran Provinces, Northern Iran
title_full The Larval Stages of Echinostoma spp. in Freshwater Snails as the First and Second Intermediate Hosts in Gilan and Mazandaran Provinces, Northern Iran
title_fullStr The Larval Stages of Echinostoma spp. in Freshwater Snails as the First and Second Intermediate Hosts in Gilan and Mazandaran Provinces, Northern Iran
title_full_unstemmed The Larval Stages of Echinostoma spp. in Freshwater Snails as the First and Second Intermediate Hosts in Gilan and Mazandaran Provinces, Northern Iran
title_short The Larval Stages of Echinostoma spp. in Freshwater Snails as the First and Second Intermediate Hosts in Gilan and Mazandaran Provinces, Northern Iran
title_sort larval stages of echinostoma spp. in freshwater snails as the first and second intermediate hosts in gilan and mazandaran provinces, northern iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10423910/
https://www.ncbi.nlm.nih.gov/pubmed/37583635
http://dx.doi.org/10.18502/ijpa.v18i2.13184
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