Cargando…
Effects of Carboxymethylcellulose Artificial Tears on Ocular Surface Microbiome Diversity and Composition, A Randomized Controlled Trial
PURPOSE: Carboxymethylcellulose is an artificial tear ingredient known to decrease gut microbiome diversity when ingested. This study examines the effect of carboxymethylcellulose on ocular surface microbiome diversity and composition. METHODS: Healthy adult participants without significant ophthalm...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Association for Research in Vision and Ophthalmology
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10424155/ https://www.ncbi.nlm.nih.gov/pubmed/37555738 http://dx.doi.org/10.1167/tvst.12.8.5 |
_version_ | 1785089614402289664 |
---|---|
author | Zhou, Yujia Sidhu, Gurjit S. Whitlock, Joan A. Abdelmalik, Bishoy Mayer, Zachary Li, Youlei Wang, Gary P. Steigleman, Walter A. |
author_facet | Zhou, Yujia Sidhu, Gurjit S. Whitlock, Joan A. Abdelmalik, Bishoy Mayer, Zachary Li, Youlei Wang, Gary P. Steigleman, Walter A. |
author_sort | Zhou, Yujia |
collection | PubMed |
description | PURPOSE: Carboxymethylcellulose is an artificial tear ingredient known to decrease gut microbiome diversity when ingested. This study examines the effect of carboxymethylcellulose on ocular surface microbiome diversity and composition. METHODS: Healthy adult participants without significant ophthalmic disease or concurrent carboxymethylcellulose artificial tear use were allocated randomly to take carboxymethylcellulose or control polyethylene glycol artificial tears for seven days. Conjunctival swabs were collected before and after artificial tear treatment. This trial is registered at clinicaltrials.gov (NCT05292755). Primary outcomes included abundance of bacterial taxa and microbiome diversity as measured by the Chao-1 richness estimate, Shannon's phylogenetic diversity index, and UniFrac analysis. Secondary outcomes included Ocular Surface Disease Index scores and artificial tear compliance. RESULTS: Of the 80 enrolled participants, 66 completed the trial. Neither intervention affected Chao-1 richness (analysis of variance [ANOVA], P = 0.231) or Shannon's diversity index (ANOVA, P = 0.224). Microbiome samples did not separate by time point (permutation multivariate analysis of variance [PERMANOVA], P = 0.223) or intervention group (PERMANOVA, P = 0.668). LEfSe taxonomic analysis revealed that carboxymethylcellulose depleted several taxa including Bacteroides and Lachnoclostridium, but enriched Enterobacteriaceae, Citrobacter, and Gordonia. Both interventions decreased OSDI scores (Wilcoxon signed rank test, P < 0.05), but there was no significant difference between interventions (Mann-Whitney U, P = 0.54). CONCLUSIONS: Carboxymethylcellulose artificial tears increased Actinobacteriota but decreased Bacteroides and Firmicutes bacteria. Carboxymethylcellulose artificial tears do not affect ocular surface microbiome diversity and are not significantly more effective than polyethylene glycol artificial tears for dry eye treatment. TRANSLATIONAL RELEVANCE: The 16S microbiome analysis has revealed small changes in the ocular surface microbiome associated with artificial tear use. |
format | Online Article Text |
id | pubmed-10424155 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Association for Research in Vision and Ophthalmology |
record_format | MEDLINE/PubMed |
spelling | pubmed-104241552023-08-15 Effects of Carboxymethylcellulose Artificial Tears on Ocular Surface Microbiome Diversity and Composition, A Randomized Controlled Trial Zhou, Yujia Sidhu, Gurjit S. Whitlock, Joan A. Abdelmalik, Bishoy Mayer, Zachary Li, Youlei Wang, Gary P. Steigleman, Walter A. Transl Vis Sci Technol Clinical Trials PURPOSE: Carboxymethylcellulose is an artificial tear ingredient known to decrease gut microbiome diversity when ingested. This study examines the effect of carboxymethylcellulose on ocular surface microbiome diversity and composition. METHODS: Healthy adult participants without significant ophthalmic disease or concurrent carboxymethylcellulose artificial tear use were allocated randomly to take carboxymethylcellulose or control polyethylene glycol artificial tears for seven days. Conjunctival swabs were collected before and after artificial tear treatment. This trial is registered at clinicaltrials.gov (NCT05292755). Primary outcomes included abundance of bacterial taxa and microbiome diversity as measured by the Chao-1 richness estimate, Shannon's phylogenetic diversity index, and UniFrac analysis. Secondary outcomes included Ocular Surface Disease Index scores and artificial tear compliance. RESULTS: Of the 80 enrolled participants, 66 completed the trial. Neither intervention affected Chao-1 richness (analysis of variance [ANOVA], P = 0.231) or Shannon's diversity index (ANOVA, P = 0.224). Microbiome samples did not separate by time point (permutation multivariate analysis of variance [PERMANOVA], P = 0.223) or intervention group (PERMANOVA, P = 0.668). LEfSe taxonomic analysis revealed that carboxymethylcellulose depleted several taxa including Bacteroides and Lachnoclostridium, but enriched Enterobacteriaceae, Citrobacter, and Gordonia. Both interventions decreased OSDI scores (Wilcoxon signed rank test, P < 0.05), but there was no significant difference between interventions (Mann-Whitney U, P = 0.54). CONCLUSIONS: Carboxymethylcellulose artificial tears increased Actinobacteriota but decreased Bacteroides and Firmicutes bacteria. Carboxymethylcellulose artificial tears do not affect ocular surface microbiome diversity and are not significantly more effective than polyethylene glycol artificial tears for dry eye treatment. TRANSLATIONAL RELEVANCE: The 16S microbiome analysis has revealed small changes in the ocular surface microbiome associated with artificial tear use. The Association for Research in Vision and Ophthalmology 2023-08-09 /pmc/articles/PMC10424155/ /pubmed/37555738 http://dx.doi.org/10.1167/tvst.12.8.5 Text en Copyright 2023 The Authors https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License. |
spellingShingle | Clinical Trials Zhou, Yujia Sidhu, Gurjit S. Whitlock, Joan A. Abdelmalik, Bishoy Mayer, Zachary Li, Youlei Wang, Gary P. Steigleman, Walter A. Effects of Carboxymethylcellulose Artificial Tears on Ocular Surface Microbiome Diversity and Composition, A Randomized Controlled Trial |
title | Effects of Carboxymethylcellulose Artificial Tears on Ocular Surface Microbiome Diversity and Composition, A Randomized Controlled Trial |
title_full | Effects of Carboxymethylcellulose Artificial Tears on Ocular Surface Microbiome Diversity and Composition, A Randomized Controlled Trial |
title_fullStr | Effects of Carboxymethylcellulose Artificial Tears on Ocular Surface Microbiome Diversity and Composition, A Randomized Controlled Trial |
title_full_unstemmed | Effects of Carboxymethylcellulose Artificial Tears on Ocular Surface Microbiome Diversity and Composition, A Randomized Controlled Trial |
title_short | Effects of Carboxymethylcellulose Artificial Tears on Ocular Surface Microbiome Diversity and Composition, A Randomized Controlled Trial |
title_sort | effects of carboxymethylcellulose artificial tears on ocular surface microbiome diversity and composition, a randomized controlled trial |
topic | Clinical Trials |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10424155/ https://www.ncbi.nlm.nih.gov/pubmed/37555738 http://dx.doi.org/10.1167/tvst.12.8.5 |
work_keys_str_mv | AT zhouyujia effectsofcarboxymethylcelluloseartificialtearsonocularsurfacemicrobiomediversityandcompositionarandomizedcontrolledtrial AT sidhugurjits effectsofcarboxymethylcelluloseartificialtearsonocularsurfacemicrobiomediversityandcompositionarandomizedcontrolledtrial AT whitlockjoana effectsofcarboxymethylcelluloseartificialtearsonocularsurfacemicrobiomediversityandcompositionarandomizedcontrolledtrial AT abdelmalikbishoy effectsofcarboxymethylcelluloseartificialtearsonocularsurfacemicrobiomediversityandcompositionarandomizedcontrolledtrial AT mayerzachary effectsofcarboxymethylcelluloseartificialtearsonocularsurfacemicrobiomediversityandcompositionarandomizedcontrolledtrial AT liyoulei effectsofcarboxymethylcelluloseartificialtearsonocularsurfacemicrobiomediversityandcompositionarandomizedcontrolledtrial AT wanggaryp effectsofcarboxymethylcelluloseartificialtearsonocularsurfacemicrobiomediversityandcompositionarandomizedcontrolledtrial AT steiglemanwaltera effectsofcarboxymethylcelluloseartificialtearsonocularsurfacemicrobiomediversityandcompositionarandomizedcontrolledtrial |