Arabidopsis NOTCHLESS plays an important role in root and embryo development

Ribosome biogenesis is a fundamental process in eukaryotic cells. NOTCHLESS (NLE) is involved in 60S ribosome biogenesis in yeast, but its role in Arabidopsis (A. thaliana) remains exclusive. Here, we found that Arabidopsis NLE (AtNLE) is highly conservative in phylogeny, which encoding a WD40-repea...

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Autores principales: Li, Ke, Zhang, Qingtian, Liu, Huiping, Wang, Fengxia, Li, Ao, Ding, Tingting, Mu, Qian, Zhao, Hongjun, Wang, Pengfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2023
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10424599/
https://www.ncbi.nlm.nih.gov/pubmed/37573563
http://dx.doi.org/10.1080/15592324.2023.2245616
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author Li, Ke
Zhang, Qingtian
Liu, Huiping
Wang, Fengxia
Li, Ao
Ding, Tingting
Mu, Qian
Zhao, Hongjun
Wang, Pengfei
author_facet Li, Ke
Zhang, Qingtian
Liu, Huiping
Wang, Fengxia
Li, Ao
Ding, Tingting
Mu, Qian
Zhao, Hongjun
Wang, Pengfei
author_sort Li, Ke
collection PubMed
description Ribosome biogenesis is a fundamental process in eukaryotic cells. NOTCHLESS (NLE) is involved in 60S ribosome biogenesis in yeast, but its role in Arabidopsis (A. thaliana) remains exclusive. Here, we found that Arabidopsis NLE (AtNLE) is highly conservative in phylogeny, which encoding a WD40-repeat protein. AtNLE is expressed in actively dividing tissues. AtNLE-GFP is localized in the nucleus. AtNLE physically interacts with the MIDAS domain of AtMDN1, a protein involved in the biogenesis of the 60S ribosomal subunit in Arabidopsis. The underexpressing mutant nle-2 shows short roots and reduced cell number in the root meristem. In addition, the null mutant nle-1 is embryo lethal, and defective embryos are arrested at the early globular stage. This work suggests that AtNLE interacts with AtMDN1, and AtNLE functions in root and embryo development.
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spelling pubmed-104245992023-08-15 Arabidopsis NOTCHLESS plays an important role in root and embryo development Li, Ke Zhang, Qingtian Liu, Huiping Wang, Fengxia Li, Ao Ding, Tingting Mu, Qian Zhao, Hongjun Wang, Pengfei Plant Signal Behav Research Paper Ribosome biogenesis is a fundamental process in eukaryotic cells. NOTCHLESS (NLE) is involved in 60S ribosome biogenesis in yeast, but its role in Arabidopsis (A. thaliana) remains exclusive. Here, we found that Arabidopsis NLE (AtNLE) is highly conservative in phylogeny, which encoding a WD40-repeat protein. AtNLE is expressed in actively dividing tissues. AtNLE-GFP is localized in the nucleus. AtNLE physically interacts with the MIDAS domain of AtMDN1, a protein involved in the biogenesis of the 60S ribosomal subunit in Arabidopsis. The underexpressing mutant nle-2 shows short roots and reduced cell number in the root meristem. In addition, the null mutant nle-1 is embryo lethal, and defective embryos are arrested at the early globular stage. This work suggests that AtNLE interacts with AtMDN1, and AtNLE functions in root and embryo development. Taylor & Francis 2023-08-13 /pmc/articles/PMC10424599/ /pubmed/37573563 http://dx.doi.org/10.1080/15592324.2023.2245616 Text en © 2023 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.
spellingShingle Research Paper
Li, Ke
Zhang, Qingtian
Liu, Huiping
Wang, Fengxia
Li, Ao
Ding, Tingting
Mu, Qian
Zhao, Hongjun
Wang, Pengfei
Arabidopsis NOTCHLESS plays an important role in root and embryo development
title Arabidopsis NOTCHLESS plays an important role in root and embryo development
title_full Arabidopsis NOTCHLESS plays an important role in root and embryo development
title_fullStr Arabidopsis NOTCHLESS plays an important role in root and embryo development
title_full_unstemmed Arabidopsis NOTCHLESS plays an important role in root and embryo development
title_short Arabidopsis NOTCHLESS plays an important role in root and embryo development
title_sort arabidopsis notchless plays an important role in root and embryo development
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10424599/
https://www.ncbi.nlm.nih.gov/pubmed/37573563
http://dx.doi.org/10.1080/15592324.2023.2245616
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