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Luteolin regulates the distribution and function of organelles by controlling SIRT1 activity during postovulatory oocyte aging

The quality of oocytes determines their development competence, which will be rapidly lost if the oocytes are not fertilized at the proper time after ovulation. SIRT1, one of the sirtuin family members, has been proven to protect the quality of oocytes during postovulatory oocyte aging. However, evi...

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Autores principales: Xing, Xupeng, Peng, Jingfeng, Zhao, Jingyu, Shi, Ruoxi, Wang, Caiqin, Zhang, Zihan, Wang, Zihan, Li, Zicong, Wu, Zhenfang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10424794/
https://www.ncbi.nlm.nih.gov/pubmed/37583461
http://dx.doi.org/10.3389/fnut.2023.1192758
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author Xing, Xupeng
Peng, Jingfeng
Zhao, Jingyu
Shi, Ruoxi
Wang, Caiqin
Zhang, Zihan
Wang, Zihan
Li, Zicong
Wu, Zhenfang
author_facet Xing, Xupeng
Peng, Jingfeng
Zhao, Jingyu
Shi, Ruoxi
Wang, Caiqin
Zhang, Zihan
Wang, Zihan
Li, Zicong
Wu, Zhenfang
author_sort Xing, Xupeng
collection PubMed
description The quality of oocytes determines their development competence, which will be rapidly lost if the oocytes are not fertilized at the proper time after ovulation. SIRT1, one of the sirtuin family members, has been proven to protect the quality of oocytes during postovulatory oocyte aging. However, evidence of the effect of SIRT1 on the activity of organelles including the mitochondria, the endoplasmic reticulum (ER), the Golgi apparatus, and the lysosomes in postovulatory aging oocyte is lacking. In this study, we investigated the distribution and function of organelles in postovulatory aged oocytes and discovered abnormalities. Luteolin, which is a natural flavonoid contained in vegetables and fruits, is an activator of SIRT1. When the oocytes were treated with luteolin, the abnormal distribution of mitochondria, ER, and Golgi complex were restored during postovulatory oocyte aging. The ER stress protein GRP78 and the lysosome protein LAMP1 increased, while the mitochondrial membrane potential and the Golgi complex protein GOLPH3 decreased in aged oocytes, and these were restored by luteolin treatment. EX-527, an inhibitor of SIRT1, disrupted the luteolin-mediated normal distribution and function of mitochondria, ER, Golgi apparatus, and lysosomes. In conclusion, we demonstrate that luteolin regulates the distribution and function of mitochondria, ER, Golgi apparatus, and lysosomes during postovulatory oocyte aging by activating SIRT1.
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spelling pubmed-104247942023-08-15 Luteolin regulates the distribution and function of organelles by controlling SIRT1 activity during postovulatory oocyte aging Xing, Xupeng Peng, Jingfeng Zhao, Jingyu Shi, Ruoxi Wang, Caiqin Zhang, Zihan Wang, Zihan Li, Zicong Wu, Zhenfang Front Nutr Nutrition The quality of oocytes determines their development competence, which will be rapidly lost if the oocytes are not fertilized at the proper time after ovulation. SIRT1, one of the sirtuin family members, has been proven to protect the quality of oocytes during postovulatory oocyte aging. However, evidence of the effect of SIRT1 on the activity of organelles including the mitochondria, the endoplasmic reticulum (ER), the Golgi apparatus, and the lysosomes in postovulatory aging oocyte is lacking. In this study, we investigated the distribution and function of organelles in postovulatory aged oocytes and discovered abnormalities. Luteolin, which is a natural flavonoid contained in vegetables and fruits, is an activator of SIRT1. When the oocytes were treated with luteolin, the abnormal distribution of mitochondria, ER, and Golgi complex were restored during postovulatory oocyte aging. The ER stress protein GRP78 and the lysosome protein LAMP1 increased, while the mitochondrial membrane potential and the Golgi complex protein GOLPH3 decreased in aged oocytes, and these were restored by luteolin treatment. EX-527, an inhibitor of SIRT1, disrupted the luteolin-mediated normal distribution and function of mitochondria, ER, Golgi apparatus, and lysosomes. In conclusion, we demonstrate that luteolin regulates the distribution and function of mitochondria, ER, Golgi apparatus, and lysosomes during postovulatory oocyte aging by activating SIRT1. Frontiers Media S.A. 2023-07-31 /pmc/articles/PMC10424794/ /pubmed/37583461 http://dx.doi.org/10.3389/fnut.2023.1192758 Text en Copyright © 2023 Xing, Peng, Zhao, Shi, Wang, Zhang, Wang, Li and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Xing, Xupeng
Peng, Jingfeng
Zhao, Jingyu
Shi, Ruoxi
Wang, Caiqin
Zhang, Zihan
Wang, Zihan
Li, Zicong
Wu, Zhenfang
Luteolin regulates the distribution and function of organelles by controlling SIRT1 activity during postovulatory oocyte aging
title Luteolin regulates the distribution and function of organelles by controlling SIRT1 activity during postovulatory oocyte aging
title_full Luteolin regulates the distribution and function of organelles by controlling SIRT1 activity during postovulatory oocyte aging
title_fullStr Luteolin regulates the distribution and function of organelles by controlling SIRT1 activity during postovulatory oocyte aging
title_full_unstemmed Luteolin regulates the distribution and function of organelles by controlling SIRT1 activity during postovulatory oocyte aging
title_short Luteolin regulates the distribution and function of organelles by controlling SIRT1 activity during postovulatory oocyte aging
title_sort luteolin regulates the distribution and function of organelles by controlling sirt1 activity during postovulatory oocyte aging
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10424794/
https://www.ncbi.nlm.nih.gov/pubmed/37583461
http://dx.doi.org/10.3389/fnut.2023.1192758
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