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Luteolin regulates the distribution and function of organelles by controlling SIRT1 activity during postovulatory oocyte aging
The quality of oocytes determines their development competence, which will be rapidly lost if the oocytes are not fertilized at the proper time after ovulation. SIRT1, one of the sirtuin family members, has been proven to protect the quality of oocytes during postovulatory oocyte aging. However, evi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10424794/ https://www.ncbi.nlm.nih.gov/pubmed/37583461 http://dx.doi.org/10.3389/fnut.2023.1192758 |
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author | Xing, Xupeng Peng, Jingfeng Zhao, Jingyu Shi, Ruoxi Wang, Caiqin Zhang, Zihan Wang, Zihan Li, Zicong Wu, Zhenfang |
author_facet | Xing, Xupeng Peng, Jingfeng Zhao, Jingyu Shi, Ruoxi Wang, Caiqin Zhang, Zihan Wang, Zihan Li, Zicong Wu, Zhenfang |
author_sort | Xing, Xupeng |
collection | PubMed |
description | The quality of oocytes determines their development competence, which will be rapidly lost if the oocytes are not fertilized at the proper time after ovulation. SIRT1, one of the sirtuin family members, has been proven to protect the quality of oocytes during postovulatory oocyte aging. However, evidence of the effect of SIRT1 on the activity of organelles including the mitochondria, the endoplasmic reticulum (ER), the Golgi apparatus, and the lysosomes in postovulatory aging oocyte is lacking. In this study, we investigated the distribution and function of organelles in postovulatory aged oocytes and discovered abnormalities. Luteolin, which is a natural flavonoid contained in vegetables and fruits, is an activator of SIRT1. When the oocytes were treated with luteolin, the abnormal distribution of mitochondria, ER, and Golgi complex were restored during postovulatory oocyte aging. The ER stress protein GRP78 and the lysosome protein LAMP1 increased, while the mitochondrial membrane potential and the Golgi complex protein GOLPH3 decreased in aged oocytes, and these were restored by luteolin treatment. EX-527, an inhibitor of SIRT1, disrupted the luteolin-mediated normal distribution and function of mitochondria, ER, Golgi apparatus, and lysosomes. In conclusion, we demonstrate that luteolin regulates the distribution and function of mitochondria, ER, Golgi apparatus, and lysosomes during postovulatory oocyte aging by activating SIRT1. |
format | Online Article Text |
id | pubmed-10424794 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-104247942023-08-15 Luteolin regulates the distribution and function of organelles by controlling SIRT1 activity during postovulatory oocyte aging Xing, Xupeng Peng, Jingfeng Zhao, Jingyu Shi, Ruoxi Wang, Caiqin Zhang, Zihan Wang, Zihan Li, Zicong Wu, Zhenfang Front Nutr Nutrition The quality of oocytes determines their development competence, which will be rapidly lost if the oocytes are not fertilized at the proper time after ovulation. SIRT1, one of the sirtuin family members, has been proven to protect the quality of oocytes during postovulatory oocyte aging. However, evidence of the effect of SIRT1 on the activity of organelles including the mitochondria, the endoplasmic reticulum (ER), the Golgi apparatus, and the lysosomes in postovulatory aging oocyte is lacking. In this study, we investigated the distribution and function of organelles in postovulatory aged oocytes and discovered abnormalities. Luteolin, which is a natural flavonoid contained in vegetables and fruits, is an activator of SIRT1. When the oocytes were treated with luteolin, the abnormal distribution of mitochondria, ER, and Golgi complex were restored during postovulatory oocyte aging. The ER stress protein GRP78 and the lysosome protein LAMP1 increased, while the mitochondrial membrane potential and the Golgi complex protein GOLPH3 decreased in aged oocytes, and these were restored by luteolin treatment. EX-527, an inhibitor of SIRT1, disrupted the luteolin-mediated normal distribution and function of mitochondria, ER, Golgi apparatus, and lysosomes. In conclusion, we demonstrate that luteolin regulates the distribution and function of mitochondria, ER, Golgi apparatus, and lysosomes during postovulatory oocyte aging by activating SIRT1. Frontiers Media S.A. 2023-07-31 /pmc/articles/PMC10424794/ /pubmed/37583461 http://dx.doi.org/10.3389/fnut.2023.1192758 Text en Copyright © 2023 Xing, Peng, Zhao, Shi, Wang, Zhang, Wang, Li and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Nutrition Xing, Xupeng Peng, Jingfeng Zhao, Jingyu Shi, Ruoxi Wang, Caiqin Zhang, Zihan Wang, Zihan Li, Zicong Wu, Zhenfang Luteolin regulates the distribution and function of organelles by controlling SIRT1 activity during postovulatory oocyte aging |
title | Luteolin regulates the distribution and function of organelles by controlling SIRT1 activity during postovulatory oocyte aging |
title_full | Luteolin regulates the distribution and function of organelles by controlling SIRT1 activity during postovulatory oocyte aging |
title_fullStr | Luteolin regulates the distribution and function of organelles by controlling SIRT1 activity during postovulatory oocyte aging |
title_full_unstemmed | Luteolin regulates the distribution and function of organelles by controlling SIRT1 activity during postovulatory oocyte aging |
title_short | Luteolin regulates the distribution and function of organelles by controlling SIRT1 activity during postovulatory oocyte aging |
title_sort | luteolin regulates the distribution and function of organelles by controlling sirt1 activity during postovulatory oocyte aging |
topic | Nutrition |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10424794/ https://www.ncbi.nlm.nih.gov/pubmed/37583461 http://dx.doi.org/10.3389/fnut.2023.1192758 |
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