Cargando…
Study on the cytokines related to SARS-Cov-2 in testicular cells and the interaction network between cells based on scRNA-seq data
Inflammatory cytokine storms (CS) in COVID-19 patients are associated with elevated levels of 13 specific cytokines, potentially impacting male fertility by causing testicular cell damage and disrupting the immune microenvironment. Some patients present with scrotal discomfort and orchitis. However,...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
De Gruyter
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10426268/ https://www.ncbi.nlm.nih.gov/pubmed/37589002 http://dx.doi.org/10.1515/biol-2022-0661 |
_version_ | 1785090019956883456 |
---|---|
author | Yu, Fan He, Haihong Huang, Tingting Zhou, Yiwen |
author_facet | Yu, Fan He, Haihong Huang, Tingting Zhou, Yiwen |
author_sort | Yu, Fan |
collection | PubMed |
description | Inflammatory cytokine storms (CS) in COVID-19 patients are associated with elevated levels of 13 specific cytokines, potentially impacting male fertility by causing testicular cell damage and disrupting the immune microenvironment. Some patients present with scrotal discomfort and orchitis. However, few studies have explored cytokine expression in testicular cells and their role in cell-to-cell communication. In this study, we integrated single-cell sequencing data sets of testicular cells, annotating 20 cell clusters using marker genes and the Human Cell Landscape database. We constructed cell pseudo-chronological trajectories, hub genes, and analyzed the cytokine interaction network between sperm cells using CellChat. Our findings identified 12 types of testicular cells, with four cytokines (IL8, CCL2, CCL3, and TNF) potentially involved in immune processes. Pseudo-chronological trajectory analysis indicated IL8 and CCL3's essential roles in testicular macrophages and endothelial cell development, affecting the immune microenvironment. We determined eight key cytokines (IL1, IL2, IL4, IL6, CCL, CSF3, TNF, and IFN-II) functions in cell interaction networks. Network analysis of exogenous cytokines directly acting on testicular cells showed IL2 potentially affecting all testicular cells, suggesting a vital role in cell communication. This research offers valuable insights into CSs effects on testicular cells and their potential impact on male fertility during COVID-19 infection. |
format | Online Article Text |
id | pubmed-10426268 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | De Gruyter |
record_format | MEDLINE/PubMed |
spelling | pubmed-104262682023-08-16 Study on the cytokines related to SARS-Cov-2 in testicular cells and the interaction network between cells based on scRNA-seq data Yu, Fan He, Haihong Huang, Tingting Zhou, Yiwen Open Life Sci Research Article Inflammatory cytokine storms (CS) in COVID-19 patients are associated with elevated levels of 13 specific cytokines, potentially impacting male fertility by causing testicular cell damage and disrupting the immune microenvironment. Some patients present with scrotal discomfort and orchitis. However, few studies have explored cytokine expression in testicular cells and their role in cell-to-cell communication. In this study, we integrated single-cell sequencing data sets of testicular cells, annotating 20 cell clusters using marker genes and the Human Cell Landscape database. We constructed cell pseudo-chronological trajectories, hub genes, and analyzed the cytokine interaction network between sperm cells using CellChat. Our findings identified 12 types of testicular cells, with four cytokines (IL8, CCL2, CCL3, and TNF) potentially involved in immune processes. Pseudo-chronological trajectory analysis indicated IL8 and CCL3's essential roles in testicular macrophages and endothelial cell development, affecting the immune microenvironment. We determined eight key cytokines (IL1, IL2, IL4, IL6, CCL, CSF3, TNF, and IFN-II) functions in cell interaction networks. Network analysis of exogenous cytokines directly acting on testicular cells showed IL2 potentially affecting all testicular cells, suggesting a vital role in cell communication. This research offers valuable insights into CSs effects on testicular cells and their potential impact on male fertility during COVID-19 infection. De Gruyter 2023-08-08 /pmc/articles/PMC10426268/ /pubmed/37589002 http://dx.doi.org/10.1515/biol-2022-0661 Text en © 2023 the author(s), published by De Gruyter https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License. |
spellingShingle | Research Article Yu, Fan He, Haihong Huang, Tingting Zhou, Yiwen Study on the cytokines related to SARS-Cov-2 in testicular cells and the interaction network between cells based on scRNA-seq data |
title | Study on the cytokines related to SARS-Cov-2 in testicular cells and the interaction network between cells based on scRNA-seq data |
title_full | Study on the cytokines related to SARS-Cov-2 in testicular cells and the interaction network between cells based on scRNA-seq data |
title_fullStr | Study on the cytokines related to SARS-Cov-2 in testicular cells and the interaction network between cells based on scRNA-seq data |
title_full_unstemmed | Study on the cytokines related to SARS-Cov-2 in testicular cells and the interaction network between cells based on scRNA-seq data |
title_short | Study on the cytokines related to SARS-Cov-2 in testicular cells and the interaction network between cells based on scRNA-seq data |
title_sort | study on the cytokines related to sars-cov-2 in testicular cells and the interaction network between cells based on scrna-seq data |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10426268/ https://www.ncbi.nlm.nih.gov/pubmed/37589002 http://dx.doi.org/10.1515/biol-2022-0661 |
work_keys_str_mv | AT yufan studyonthecytokinesrelatedtosarscov2intesticularcellsandtheinteractionnetworkbetweencellsbasedonscrnaseqdata AT hehaihong studyonthecytokinesrelatedtosarscov2intesticularcellsandtheinteractionnetworkbetweencellsbasedonscrnaseqdata AT huangtingting studyonthecytokinesrelatedtosarscov2intesticularcellsandtheinteractionnetworkbetweencellsbasedonscrnaseqdata AT zhouyiwen studyonthecytokinesrelatedtosarscov2intesticularcellsandtheinteractionnetworkbetweencellsbasedonscrnaseqdata |