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Urinary titin is not an early biomarker of skeletal muscle atrophy induced by muscle denervation in mice

Early detection of skeletal muscle atrophy is important to prevent further muscle weakness. However, there are few non-invasive biomarkers for skeletal muscle atrophy. Recent studies have reported that the N-terminal fragment (N-titin) of titin, a giant sarcomeric protein, is detected in the urine o...

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Autores principales: Tanihata, Jun, Minamisawa, Susumu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10426992/
https://www.ncbi.nlm.nih.gov/pubmed/37582074
http://dx.doi.org/10.1371/journal.pone.0289185
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author Tanihata, Jun
Minamisawa, Susumu
author_facet Tanihata, Jun
Minamisawa, Susumu
author_sort Tanihata, Jun
collection PubMed
description Early detection of skeletal muscle atrophy is important to prevent further muscle weakness. However, there are few non-invasive biomarkers for skeletal muscle atrophy. Recent studies have reported that the N-terminal fragment (N-titin) of titin, a giant sarcomeric protein, is detected in the urine of patients with muscle damage. In this study, we hypothesized that urinary N-titin would be a potential early biomarker of skeletal muscle atrophy in mice caused by sciatic nerve denervation. Male mice were randomly divided into control and denervation groups, and urinary N-titin levels were assessed daily for 9 days using an enzyme-linked immunosorbent assay system. Despite reduced titin protein levels in atrophic muscles 10 days after denervation, cleaved N-titin fragments were not increased in the urine of mice with denervation-induced muscle atrophy. Furthermore, we found no uptake of Evans blue dye from the extracellular space into the cytoplasm in atrophic muscles, suggesting that the sarcomeric membrane is intact in those muscles. The present results suggest that cleaved N-titin in the urine is not suitable as an early biomarker of skeletal muscle atrophy.
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spelling pubmed-104269922023-08-16 Urinary titin is not an early biomarker of skeletal muscle atrophy induced by muscle denervation in mice Tanihata, Jun Minamisawa, Susumu PLoS One Research Article Early detection of skeletal muscle atrophy is important to prevent further muscle weakness. However, there are few non-invasive biomarkers for skeletal muscle atrophy. Recent studies have reported that the N-terminal fragment (N-titin) of titin, a giant sarcomeric protein, is detected in the urine of patients with muscle damage. In this study, we hypothesized that urinary N-titin would be a potential early biomarker of skeletal muscle atrophy in mice caused by sciatic nerve denervation. Male mice were randomly divided into control and denervation groups, and urinary N-titin levels were assessed daily for 9 days using an enzyme-linked immunosorbent assay system. Despite reduced titin protein levels in atrophic muscles 10 days after denervation, cleaved N-titin fragments were not increased in the urine of mice with denervation-induced muscle atrophy. Furthermore, we found no uptake of Evans blue dye from the extracellular space into the cytoplasm in atrophic muscles, suggesting that the sarcomeric membrane is intact in those muscles. The present results suggest that cleaved N-titin in the urine is not suitable as an early biomarker of skeletal muscle atrophy. Public Library of Science 2023-08-15 /pmc/articles/PMC10426992/ /pubmed/37582074 http://dx.doi.org/10.1371/journal.pone.0289185 Text en © 2023 Tanihata, Minamisawa https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Tanihata, Jun
Minamisawa, Susumu
Urinary titin is not an early biomarker of skeletal muscle atrophy induced by muscle denervation in mice
title Urinary titin is not an early biomarker of skeletal muscle atrophy induced by muscle denervation in mice
title_full Urinary titin is not an early biomarker of skeletal muscle atrophy induced by muscle denervation in mice
title_fullStr Urinary titin is not an early biomarker of skeletal muscle atrophy induced by muscle denervation in mice
title_full_unstemmed Urinary titin is not an early biomarker of skeletal muscle atrophy induced by muscle denervation in mice
title_short Urinary titin is not an early biomarker of skeletal muscle atrophy induced by muscle denervation in mice
title_sort urinary titin is not an early biomarker of skeletal muscle atrophy induced by muscle denervation in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10426992/
https://www.ncbi.nlm.nih.gov/pubmed/37582074
http://dx.doi.org/10.1371/journal.pone.0289185
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