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Development of DNA markers using next-generation sequencing approach for molecular authentication of Boerhavia diffusa L. and Tinospora cordifolia (Willd.) Miers

The adulteration of plants and their materials used in herbal formulations poses a severe health concern. Hence, there is a need to establish a reliable, cost-effective, and robust molecular biomarker to distinguish among species and identify herbal plants and raw drugs from adulterants. The present...

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Autores principales: Sharma, Anu Radha, Vohra, Manik, Vinay, Chigateri M., Paul, Bobby, Chakrabarty, Sanjiban, Rai, Padmalatha S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10427588/
https://www.ncbi.nlm.nih.gov/pubmed/37593204
http://dx.doi.org/10.1007/s13205-023-03732-7
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author Sharma, Anu Radha
Vohra, Manik
Vinay, Chigateri M.
Paul, Bobby
Chakrabarty, Sanjiban
Rai, Padmalatha S.
author_facet Sharma, Anu Radha
Vohra, Manik
Vinay, Chigateri M.
Paul, Bobby
Chakrabarty, Sanjiban
Rai, Padmalatha S.
author_sort Sharma, Anu Radha
collection PubMed
description The adulteration of plants and their materials used in herbal formulations poses a severe health concern. Hence, there is a need to establish a reliable, cost-effective, and robust molecular biomarker to distinguish among species and identify herbal plants and raw drugs from adulterants. The present study used suppressive subtractive hybridization and next-generation sequencing technology to identify novel DNA markers for Boerhavia diffusa L. and Tinospora cordifolia (Willd.) Miers. We identified two primer sets for B. diffusa and one for T. cordifolia. The DNA markers were validated in different accessions of B. diffusa and T. cordifolia and their common adulterants to determine the sensitivity and specificity of developed DNA markers. The designed DNA markers showed 100% sensitivity and specificity in detecting B. diffusa and T. cordifolia from their adulterants. The strategy described here can be extrapolated for developing DNA markers to authenticate other plant species. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13205-023-03732-7.
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spelling pubmed-104275882023-08-17 Development of DNA markers using next-generation sequencing approach for molecular authentication of Boerhavia diffusa L. and Tinospora cordifolia (Willd.) Miers Sharma, Anu Radha Vohra, Manik Vinay, Chigateri M. Paul, Bobby Chakrabarty, Sanjiban Rai, Padmalatha S. 3 Biotech Original Article The adulteration of plants and their materials used in herbal formulations poses a severe health concern. Hence, there is a need to establish a reliable, cost-effective, and robust molecular biomarker to distinguish among species and identify herbal plants and raw drugs from adulterants. The present study used suppressive subtractive hybridization and next-generation sequencing technology to identify novel DNA markers for Boerhavia diffusa L. and Tinospora cordifolia (Willd.) Miers. We identified two primer sets for B. diffusa and one for T. cordifolia. The DNA markers were validated in different accessions of B. diffusa and T. cordifolia and their common adulterants to determine the sensitivity and specificity of developed DNA markers. The designed DNA markers showed 100% sensitivity and specificity in detecting B. diffusa and T. cordifolia from their adulterants. The strategy described here can be extrapolated for developing DNA markers to authenticate other plant species. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13205-023-03732-7. Springer International Publishing 2023-08-15 2023-09 /pmc/articles/PMC10427588/ /pubmed/37593204 http://dx.doi.org/10.1007/s13205-023-03732-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Sharma, Anu Radha
Vohra, Manik
Vinay, Chigateri M.
Paul, Bobby
Chakrabarty, Sanjiban
Rai, Padmalatha S.
Development of DNA markers using next-generation sequencing approach for molecular authentication of Boerhavia diffusa L. and Tinospora cordifolia (Willd.) Miers
title Development of DNA markers using next-generation sequencing approach for molecular authentication of Boerhavia diffusa L. and Tinospora cordifolia (Willd.) Miers
title_full Development of DNA markers using next-generation sequencing approach for molecular authentication of Boerhavia diffusa L. and Tinospora cordifolia (Willd.) Miers
title_fullStr Development of DNA markers using next-generation sequencing approach for molecular authentication of Boerhavia diffusa L. and Tinospora cordifolia (Willd.) Miers
title_full_unstemmed Development of DNA markers using next-generation sequencing approach for molecular authentication of Boerhavia diffusa L. and Tinospora cordifolia (Willd.) Miers
title_short Development of DNA markers using next-generation sequencing approach for molecular authentication of Boerhavia diffusa L. and Tinospora cordifolia (Willd.) Miers
title_sort development of dna markers using next-generation sequencing approach for molecular authentication of boerhavia diffusa l. and tinospora cordifolia (willd.) miers
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10427588/
https://www.ncbi.nlm.nih.gov/pubmed/37593204
http://dx.doi.org/10.1007/s13205-023-03732-7
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