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Propionic acid induces alterations in mitochondrial morphology and dynamics in SH-SY5Y cells
Propionic acid (PPA) is used to study the role of mitochondrial dysfunction in neurodevelopmental conditions like autism spectrum disorders. PPA is known to disrupt mitochondrial biogenesis, metabolism, and turnover. However, the effect of PPA on mitochondrial dynamics, fission, and fusion remains c...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10427685/ https://www.ncbi.nlm.nih.gov/pubmed/37582965 http://dx.doi.org/10.1038/s41598-023-40130-8 |
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author | Buchanan, Erin Mahony, Caitlyn Bam, Sophia Jaffer, Mohamed Macleod, Sarah Mangali, Asandile van der Watt, Mignon de Wet, Sholto Theart, Rensu Jacobs, Caron Loos, Ben O’Ryan, Colleen |
author_facet | Buchanan, Erin Mahony, Caitlyn Bam, Sophia Jaffer, Mohamed Macleod, Sarah Mangali, Asandile van der Watt, Mignon de Wet, Sholto Theart, Rensu Jacobs, Caron Loos, Ben O’Ryan, Colleen |
author_sort | Buchanan, Erin |
collection | PubMed |
description | Propionic acid (PPA) is used to study the role of mitochondrial dysfunction in neurodevelopmental conditions like autism spectrum disorders. PPA is known to disrupt mitochondrial biogenesis, metabolism, and turnover. However, the effect of PPA on mitochondrial dynamics, fission, and fusion remains challenging to study due to the complex temporal nature of these mechanisms. Here, we use complementary quantitative visualization techniques to examine how PPA influences mitochondrial ultrastructure, morphology, and dynamics in neuronal-like SH-SY5Y cells. PPA (5 mM) induced a significant decrease in mitochondrial area (p < 0.01), Feret's diameter and perimeter (p < 0.05), and in area(2) (p < 0.01). Mitochondrial event localiser analysis demonstrated a significant increase in fission and fusion events (p < 0.05) that preserved mitochondrial network integrity under stress. Moreover, mRNA expression of cMYC (p < 0.0001), NRF1 (p < 0.01), TFAM (p < 0.05), STOML2 (p < 0.0001), and OPA1 (p < 0.01) was significantly decreased. This illustrates a remodeling of mitochondrial morphology, biogenesis, and dynamics to preserve function under stress. Our data provide new insights into the influence of PPA on mitochondrial dynamics and highlight the utility of visualization techniques to study the complex regulatory mechanisms involved in the mitochondrial stress response. |
format | Online Article Text |
id | pubmed-10427685 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-104276852023-08-17 Propionic acid induces alterations in mitochondrial morphology and dynamics in SH-SY5Y cells Buchanan, Erin Mahony, Caitlyn Bam, Sophia Jaffer, Mohamed Macleod, Sarah Mangali, Asandile van der Watt, Mignon de Wet, Sholto Theart, Rensu Jacobs, Caron Loos, Ben O’Ryan, Colleen Sci Rep Article Propionic acid (PPA) is used to study the role of mitochondrial dysfunction in neurodevelopmental conditions like autism spectrum disorders. PPA is known to disrupt mitochondrial biogenesis, metabolism, and turnover. However, the effect of PPA on mitochondrial dynamics, fission, and fusion remains challenging to study due to the complex temporal nature of these mechanisms. Here, we use complementary quantitative visualization techniques to examine how PPA influences mitochondrial ultrastructure, morphology, and dynamics in neuronal-like SH-SY5Y cells. PPA (5 mM) induced a significant decrease in mitochondrial area (p < 0.01), Feret's diameter and perimeter (p < 0.05), and in area(2) (p < 0.01). Mitochondrial event localiser analysis demonstrated a significant increase in fission and fusion events (p < 0.05) that preserved mitochondrial network integrity under stress. Moreover, mRNA expression of cMYC (p < 0.0001), NRF1 (p < 0.01), TFAM (p < 0.05), STOML2 (p < 0.0001), and OPA1 (p < 0.01) was significantly decreased. This illustrates a remodeling of mitochondrial morphology, biogenesis, and dynamics to preserve function under stress. Our data provide new insights into the influence of PPA on mitochondrial dynamics and highlight the utility of visualization techniques to study the complex regulatory mechanisms involved in the mitochondrial stress response. Nature Publishing Group UK 2023-08-15 /pmc/articles/PMC10427685/ /pubmed/37582965 http://dx.doi.org/10.1038/s41598-023-40130-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Buchanan, Erin Mahony, Caitlyn Bam, Sophia Jaffer, Mohamed Macleod, Sarah Mangali, Asandile van der Watt, Mignon de Wet, Sholto Theart, Rensu Jacobs, Caron Loos, Ben O’Ryan, Colleen Propionic acid induces alterations in mitochondrial morphology and dynamics in SH-SY5Y cells |
title | Propionic acid induces alterations in mitochondrial morphology and dynamics in SH-SY5Y cells |
title_full | Propionic acid induces alterations in mitochondrial morphology and dynamics in SH-SY5Y cells |
title_fullStr | Propionic acid induces alterations in mitochondrial morphology and dynamics in SH-SY5Y cells |
title_full_unstemmed | Propionic acid induces alterations in mitochondrial morphology and dynamics in SH-SY5Y cells |
title_short | Propionic acid induces alterations in mitochondrial morphology and dynamics in SH-SY5Y cells |
title_sort | propionic acid induces alterations in mitochondrial morphology and dynamics in sh-sy5y cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10427685/ https://www.ncbi.nlm.nih.gov/pubmed/37582965 http://dx.doi.org/10.1038/s41598-023-40130-8 |
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