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Dual effectiveness of a novel all-in-one endodontic irrigating solution in antibiofilm activity and smear layer removal

The continuous destruction of dental hard tissues increases the risk of bacterial invasion, which leads to pulp infections. Irrigation is critical for successful root canal treatment in terms of infection control. However, no single irrigant covers all of the functions demanded, including antibiofil...

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Autores principales: Sheng, Xuyan, Yu, Jian, Liu, He, Wang, Zhejun, Deng, Shuli, Shen, Ya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10427723/
https://www.ncbi.nlm.nih.gov/pubmed/37593327
http://dx.doi.org/10.3389/fbioe.2023.1254927
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author Sheng, Xuyan
Yu, Jian
Liu, He
Wang, Zhejun
Deng, Shuli
Shen, Ya
author_facet Sheng, Xuyan
Yu, Jian
Liu, He
Wang, Zhejun
Deng, Shuli
Shen, Ya
author_sort Sheng, Xuyan
collection PubMed
description The continuous destruction of dental hard tissues increases the risk of bacterial invasion, which leads to pulp infections. Irrigation is critical for successful root canal treatment in terms of infection control. However, no single irrigant covers all of the functions demanded, including antibiofilm and tissue-dissolving activities. The aim of this study was to investigate the antimicrobial properties of Triton, an all-in-one irrigant, on Enterococcus faecalis and multispecies oral biofilms in dentin canals, as well as its ability to remove the smear layer. Dentin blocks (192 specimens) were prepared from single-root human teeth and then assigned to 48 groups (24 groups for each biofilm type). Serial centrifugation was used for bacterial introduction into dentinal tubules. After 3 weeks, half of the specimens were created a uniform smear layer. The following treatments were applied: short time (separate): Triton, 6% NaOCl, 2% NaOCl, and water (all for 3 min); short time (combined): Triton (3 + 1 min), 6% NaOCl +17% EDTA (3 + 1 or 2 + 1 min), and 2% NaOCl +17% EDTA (3 + 1 min); and long time: Triton (3 + 3 min), 6% NaOCl (5 min), 6% NaOCl +17% EDTA (5 + 1 min), and water (3 + 3 min). Confocal laser scanning microscopy and scanning electron microscopy were employed to examine the antimicrobial activity and smear layer removal, respectively. The results revealed that despite the absence or presence of the smear layer, Triton (3 + 3 min) showed the highest killing for both tested biofilms (61.53%–72.22%) among all groups (p < 0.05). Furthermore, the smear layer was removed by Triton after 3 + 3 min, exposing open dentin canals. These findings demonstrated that Triton can provide dual benefits of antibiofilm and smear layer removal capabilities simultaneously, indicating a simplified and effective strategy for application in root canal treatment.
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spelling pubmed-104277232023-08-17 Dual effectiveness of a novel all-in-one endodontic irrigating solution in antibiofilm activity and smear layer removal Sheng, Xuyan Yu, Jian Liu, He Wang, Zhejun Deng, Shuli Shen, Ya Front Bioeng Biotechnol Bioengineering and Biotechnology The continuous destruction of dental hard tissues increases the risk of bacterial invasion, which leads to pulp infections. Irrigation is critical for successful root canal treatment in terms of infection control. However, no single irrigant covers all of the functions demanded, including antibiofilm and tissue-dissolving activities. The aim of this study was to investigate the antimicrobial properties of Triton, an all-in-one irrigant, on Enterococcus faecalis and multispecies oral biofilms in dentin canals, as well as its ability to remove the smear layer. Dentin blocks (192 specimens) were prepared from single-root human teeth and then assigned to 48 groups (24 groups for each biofilm type). Serial centrifugation was used for bacterial introduction into dentinal tubules. After 3 weeks, half of the specimens were created a uniform smear layer. The following treatments were applied: short time (separate): Triton, 6% NaOCl, 2% NaOCl, and water (all for 3 min); short time (combined): Triton (3 + 1 min), 6% NaOCl +17% EDTA (3 + 1 or 2 + 1 min), and 2% NaOCl +17% EDTA (3 + 1 min); and long time: Triton (3 + 3 min), 6% NaOCl (5 min), 6% NaOCl +17% EDTA (5 + 1 min), and water (3 + 3 min). Confocal laser scanning microscopy and scanning electron microscopy were employed to examine the antimicrobial activity and smear layer removal, respectively. The results revealed that despite the absence or presence of the smear layer, Triton (3 + 3 min) showed the highest killing for both tested biofilms (61.53%–72.22%) among all groups (p < 0.05). Furthermore, the smear layer was removed by Triton after 3 + 3 min, exposing open dentin canals. These findings demonstrated that Triton can provide dual benefits of antibiofilm and smear layer removal capabilities simultaneously, indicating a simplified and effective strategy for application in root canal treatment. Frontiers Media S.A. 2023-08-01 /pmc/articles/PMC10427723/ /pubmed/37593327 http://dx.doi.org/10.3389/fbioe.2023.1254927 Text en Copyright © 2023 Sheng, Yu, Liu, Wang, Deng and Shen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Sheng, Xuyan
Yu, Jian
Liu, He
Wang, Zhejun
Deng, Shuli
Shen, Ya
Dual effectiveness of a novel all-in-one endodontic irrigating solution in antibiofilm activity and smear layer removal
title Dual effectiveness of a novel all-in-one endodontic irrigating solution in antibiofilm activity and smear layer removal
title_full Dual effectiveness of a novel all-in-one endodontic irrigating solution in antibiofilm activity and smear layer removal
title_fullStr Dual effectiveness of a novel all-in-one endodontic irrigating solution in antibiofilm activity and smear layer removal
title_full_unstemmed Dual effectiveness of a novel all-in-one endodontic irrigating solution in antibiofilm activity and smear layer removal
title_short Dual effectiveness of a novel all-in-one endodontic irrigating solution in antibiofilm activity and smear layer removal
title_sort dual effectiveness of a novel all-in-one endodontic irrigating solution in antibiofilm activity and smear layer removal
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10427723/
https://www.ncbi.nlm.nih.gov/pubmed/37593327
http://dx.doi.org/10.3389/fbioe.2023.1254927
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