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On the micelle formation of DNAJB6b
The human chaperone DNAJB6b increases the solubility of proteins involved in protein aggregation diseases and suppresses the nucleation of amyloid structures. Due to such favourable properties, DNAJB6b has gained increasing attention over the past decade. The understanding of how DNAJB6b operates on...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cambridge University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10427797/ https://www.ncbi.nlm.nih.gov/pubmed/37593255 http://dx.doi.org/10.1017/qrd.2023.4 |
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author | Carlsson, Andreas Olsson, Ulf Linse, Sara |
author_facet | Carlsson, Andreas Olsson, Ulf Linse, Sara |
author_sort | Carlsson, Andreas |
collection | PubMed |
description | The human chaperone DNAJB6b increases the solubility of proteins involved in protein aggregation diseases and suppresses the nucleation of amyloid structures. Due to such favourable properties, DNAJB6b has gained increasing attention over the past decade. The understanding of how DNAJB6b operates on a molecular level may aid the design of inhibitors against amyloid formation. In this work, fundamental aspects of DNAJB6b self-assembly have been examined, providing a basis for future experimental designs and conclusions. The results imply the formation of large chaperone clusters in a concentration-dependent manner. Microfluidic diffusional sizing (MDS) was used to evaluate how DNAJB6b average hydrodynamic radius varies with concentration. We found that, in 20 mM sodium phosphate buffer, 0.2 mM EDTA, at pH 8.0 and room temperature, DNAJB6b displays a micellar behaviour, with a critical micelle concentration (CMC) of around 120 nM. The average hydrodynamic radius appears to be concentration independent between ∼10 μM and 100 μM, with a mean radius of about 12 nm. The CMC found by MDS is supported by native agarose gel electrophoresis and the size distribution appears bimodal in the DNAJB6b concentration range ∼100 nM to 4 μM. |
format | Online Article Text |
id | pubmed-10427797 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cambridge University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-104277972023-08-17 On the micelle formation of DNAJB6b Carlsson, Andreas Olsson, Ulf Linse, Sara QRB Discov Research Article The human chaperone DNAJB6b increases the solubility of proteins involved in protein aggregation diseases and suppresses the nucleation of amyloid structures. Due to such favourable properties, DNAJB6b has gained increasing attention over the past decade. The understanding of how DNAJB6b operates on a molecular level may aid the design of inhibitors against amyloid formation. In this work, fundamental aspects of DNAJB6b self-assembly have been examined, providing a basis for future experimental designs and conclusions. The results imply the formation of large chaperone clusters in a concentration-dependent manner. Microfluidic diffusional sizing (MDS) was used to evaluate how DNAJB6b average hydrodynamic radius varies with concentration. We found that, in 20 mM sodium phosphate buffer, 0.2 mM EDTA, at pH 8.0 and room temperature, DNAJB6b displays a micellar behaviour, with a critical micelle concentration (CMC) of around 120 nM. The average hydrodynamic radius appears to be concentration independent between ∼10 μM and 100 μM, with a mean radius of about 12 nm. The CMC found by MDS is supported by native agarose gel electrophoresis and the size distribution appears bimodal in the DNAJB6b concentration range ∼100 nM to 4 μM. Cambridge University Press 2023-08-15 /pmc/articles/PMC10427797/ /pubmed/37593255 http://dx.doi.org/10.1017/qrd.2023.4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited. |
spellingShingle | Research Article Carlsson, Andreas Olsson, Ulf Linse, Sara On the micelle formation of DNAJB6b |
title | On the micelle formation of DNAJB6b |
title_full | On the micelle formation of DNAJB6b |
title_fullStr | On the micelle formation of DNAJB6b |
title_full_unstemmed | On the micelle formation of DNAJB6b |
title_short | On the micelle formation of DNAJB6b |
title_sort | on the micelle formation of dnajb6b |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10427797/ https://www.ncbi.nlm.nih.gov/pubmed/37593255 http://dx.doi.org/10.1017/qrd.2023.4 |
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