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Symbiotic bacteria, immune-like sentinel cells, and the response to pathogens in a social amoeba
Some endosymbionts living within a host must modulate their hosts' immune systems in order to infect and persist. We studied the effect of a bacterial endosymbiont on a facultatively multicellular social amoeba host. Aggregates of the amoeba Dictyostelium discoideum contain a subpopulation of s...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Royal Society
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10427822/ https://www.ncbi.nlm.nih.gov/pubmed/37593719 http://dx.doi.org/10.1098/rsos.230727 |
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author | Scott, Trey J. Larsen, Tyler J. Brock, Debra A. Uhm, So Yeon Stacey Queller, David C. Strassmann, Joan E. |
author_facet | Scott, Trey J. Larsen, Tyler J. Brock, Debra A. Uhm, So Yeon Stacey Queller, David C. Strassmann, Joan E. |
author_sort | Scott, Trey J. |
collection | PubMed |
description | Some endosymbionts living within a host must modulate their hosts' immune systems in order to infect and persist. We studied the effect of a bacterial endosymbiont on a facultatively multicellular social amoeba host. Aggregates of the amoeba Dictyostelium discoideum contain a subpopulation of sentinel cells that function akin to the immune systems of more conventional multicellular organisms. Sentinel cells sequester and discard toxins from D. discoideum aggregates and may play a central role in defence against pathogens. We measured the number and functionality of sentinel cells in aggregates of D. discoideum infected by bacterial endosymbionts in the genus Paraburkholderia. Infected D. discoideum produced fewer and less functional sentinel cells, suggesting that Paraburkholderia may interfere with its host's immune system. Despite impaired sentinel cells, however, infected D. discoideum were less sensitive to ethidium bromide toxicity, suggesting that Paraburkholderia may also have a protective effect on its host. By contrast, D. discoideum infected by Paraburkholderia did not show differences in their sensitivity to two non-symbiotic pathogens. Our results expand previous work on yet another aspect of the complicated relationship between D. discoideum and Paraburkholderia, which has considerable potential as a model for the study of symbiosis. |
format | Online Article Text |
id | pubmed-10427822 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-104278222023-08-17 Symbiotic bacteria, immune-like sentinel cells, and the response to pathogens in a social amoeba Scott, Trey J. Larsen, Tyler J. Brock, Debra A. Uhm, So Yeon Stacey Queller, David C. Strassmann, Joan E. R Soc Open Sci Organismal and Evolutionary Biology Some endosymbionts living within a host must modulate their hosts' immune systems in order to infect and persist. We studied the effect of a bacterial endosymbiont on a facultatively multicellular social amoeba host. Aggregates of the amoeba Dictyostelium discoideum contain a subpopulation of sentinel cells that function akin to the immune systems of more conventional multicellular organisms. Sentinel cells sequester and discard toxins from D. discoideum aggregates and may play a central role in defence against pathogens. We measured the number and functionality of sentinel cells in aggregates of D. discoideum infected by bacterial endosymbionts in the genus Paraburkholderia. Infected D. discoideum produced fewer and less functional sentinel cells, suggesting that Paraburkholderia may interfere with its host's immune system. Despite impaired sentinel cells, however, infected D. discoideum were less sensitive to ethidium bromide toxicity, suggesting that Paraburkholderia may also have a protective effect on its host. By contrast, D. discoideum infected by Paraburkholderia did not show differences in their sensitivity to two non-symbiotic pathogens. Our results expand previous work on yet another aspect of the complicated relationship between D. discoideum and Paraburkholderia, which has considerable potential as a model for the study of symbiosis. The Royal Society 2023-08-16 /pmc/articles/PMC10427822/ /pubmed/37593719 http://dx.doi.org/10.1098/rsos.230727 Text en © 2023 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Organismal and Evolutionary Biology Scott, Trey J. Larsen, Tyler J. Brock, Debra A. Uhm, So Yeon Stacey Queller, David C. Strassmann, Joan E. Symbiotic bacteria, immune-like sentinel cells, and the response to pathogens in a social amoeba |
title | Symbiotic bacteria, immune-like sentinel cells, and the response to pathogens in a social amoeba |
title_full | Symbiotic bacteria, immune-like sentinel cells, and the response to pathogens in a social amoeba |
title_fullStr | Symbiotic bacteria, immune-like sentinel cells, and the response to pathogens in a social amoeba |
title_full_unstemmed | Symbiotic bacteria, immune-like sentinel cells, and the response to pathogens in a social amoeba |
title_short | Symbiotic bacteria, immune-like sentinel cells, and the response to pathogens in a social amoeba |
title_sort | symbiotic bacteria, immune-like sentinel cells, and the response to pathogens in a social amoeba |
topic | Organismal and Evolutionary Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10427822/ https://www.ncbi.nlm.nih.gov/pubmed/37593719 http://dx.doi.org/10.1098/rsos.230727 |
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