Cargando…

Symbiotic bacteria, immune-like sentinel cells, and the response to pathogens in a social amoeba

Some endosymbionts living within a host must modulate their hosts' immune systems in order to infect and persist. We studied the effect of a bacterial endosymbiont on a facultatively multicellular social amoeba host. Aggregates of the amoeba Dictyostelium discoideum contain a subpopulation of s...

Descripción completa

Detalles Bibliográficos
Autores principales: Scott, Trey J., Larsen, Tyler J., Brock, Debra A., Uhm, So Yeon Stacey, Queller, David C., Strassmann, Joan E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10427822/
https://www.ncbi.nlm.nih.gov/pubmed/37593719
http://dx.doi.org/10.1098/rsos.230727
_version_ 1785090330457014272
author Scott, Trey J.
Larsen, Tyler J.
Brock, Debra A.
Uhm, So Yeon Stacey
Queller, David C.
Strassmann, Joan E.
author_facet Scott, Trey J.
Larsen, Tyler J.
Brock, Debra A.
Uhm, So Yeon Stacey
Queller, David C.
Strassmann, Joan E.
author_sort Scott, Trey J.
collection PubMed
description Some endosymbionts living within a host must modulate their hosts' immune systems in order to infect and persist. We studied the effect of a bacterial endosymbiont on a facultatively multicellular social amoeba host. Aggregates of the amoeba Dictyostelium discoideum contain a subpopulation of sentinel cells that function akin to the immune systems of more conventional multicellular organisms. Sentinel cells sequester and discard toxins from D. discoideum aggregates and may play a central role in defence against pathogens. We measured the number and functionality of sentinel cells in aggregates of D. discoideum infected by bacterial endosymbionts in the genus Paraburkholderia. Infected D. discoideum produced fewer and less functional sentinel cells, suggesting that Paraburkholderia may interfere with its host's immune system. Despite impaired sentinel cells, however, infected D. discoideum were less sensitive to ethidium bromide toxicity, suggesting that Paraburkholderia may also have a protective effect on its host. By contrast, D. discoideum infected by Paraburkholderia did not show differences in their sensitivity to two non-symbiotic pathogens. Our results expand previous work on yet another aspect of the complicated relationship between D. discoideum and Paraburkholderia, which has considerable potential as a model for the study of symbiosis.
format Online
Article
Text
id pubmed-10427822
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher The Royal Society
record_format MEDLINE/PubMed
spelling pubmed-104278222023-08-17 Symbiotic bacteria, immune-like sentinel cells, and the response to pathogens in a social amoeba Scott, Trey J. Larsen, Tyler J. Brock, Debra A. Uhm, So Yeon Stacey Queller, David C. Strassmann, Joan E. R Soc Open Sci Organismal and Evolutionary Biology Some endosymbionts living within a host must modulate their hosts' immune systems in order to infect and persist. We studied the effect of a bacterial endosymbiont on a facultatively multicellular social amoeba host. Aggregates of the amoeba Dictyostelium discoideum contain a subpopulation of sentinel cells that function akin to the immune systems of more conventional multicellular organisms. Sentinel cells sequester and discard toxins from D. discoideum aggregates and may play a central role in defence against pathogens. We measured the number and functionality of sentinel cells in aggregates of D. discoideum infected by bacterial endosymbionts in the genus Paraburkholderia. Infected D. discoideum produced fewer and less functional sentinel cells, suggesting that Paraburkholderia may interfere with its host's immune system. Despite impaired sentinel cells, however, infected D. discoideum were less sensitive to ethidium bromide toxicity, suggesting that Paraburkholderia may also have a protective effect on its host. By contrast, D. discoideum infected by Paraburkholderia did not show differences in their sensitivity to two non-symbiotic pathogens. Our results expand previous work on yet another aspect of the complicated relationship between D. discoideum and Paraburkholderia, which has considerable potential as a model for the study of symbiosis. The Royal Society 2023-08-16 /pmc/articles/PMC10427822/ /pubmed/37593719 http://dx.doi.org/10.1098/rsos.230727 Text en © 2023 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited.
spellingShingle Organismal and Evolutionary Biology
Scott, Trey J.
Larsen, Tyler J.
Brock, Debra A.
Uhm, So Yeon Stacey
Queller, David C.
Strassmann, Joan E.
Symbiotic bacteria, immune-like sentinel cells, and the response to pathogens in a social amoeba
title Symbiotic bacteria, immune-like sentinel cells, and the response to pathogens in a social amoeba
title_full Symbiotic bacteria, immune-like sentinel cells, and the response to pathogens in a social amoeba
title_fullStr Symbiotic bacteria, immune-like sentinel cells, and the response to pathogens in a social amoeba
title_full_unstemmed Symbiotic bacteria, immune-like sentinel cells, and the response to pathogens in a social amoeba
title_short Symbiotic bacteria, immune-like sentinel cells, and the response to pathogens in a social amoeba
title_sort symbiotic bacteria, immune-like sentinel cells, and the response to pathogens in a social amoeba
topic Organismal and Evolutionary Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10427822/
https://www.ncbi.nlm.nih.gov/pubmed/37593719
http://dx.doi.org/10.1098/rsos.230727
work_keys_str_mv AT scotttreyj symbioticbacteriaimmunelikesentinelcellsandtheresponsetopathogensinasocialamoeba
AT larsentylerj symbioticbacteriaimmunelikesentinelcellsandtheresponsetopathogensinasocialamoeba
AT brockdebraa symbioticbacteriaimmunelikesentinelcellsandtheresponsetopathogensinasocialamoeba
AT uhmsoyeonstacey symbioticbacteriaimmunelikesentinelcellsandtheresponsetopathogensinasocialamoeba
AT quellerdavidc symbioticbacteriaimmunelikesentinelcellsandtheresponsetopathogensinasocialamoeba
AT strassmannjoane symbioticbacteriaimmunelikesentinelcellsandtheresponsetopathogensinasocialamoeba