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Enhance immune response to H9 AIV DNA vaccine based on polygene expression and DGL nanoparticle encapsulation

DNA vaccination has great potential to treat or prevent avian influenza pandemics, but the technique may be limited by low immunogenicity and gene delivery in clinical testing. Here, to improve the immune efficacy of DNA vaccines against avian influenza, we prepared and tested the immunogenicity of...

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Autores principales: Xu, Shangen, Yu, Lu, Teng, Qiaoyang, Li, Xuesong, Jin, Zheng, Qu, Yang, Li, Jiawei, Zhang, Qihong, Li, Zejun, Zhao, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10428121/
https://www.ncbi.nlm.nih.gov/pubmed/37542938
http://dx.doi.org/10.1016/j.psj.2023.102925
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author Xu, Shangen
Yu, Lu
Teng, Qiaoyang
Li, Xuesong
Jin, Zheng
Qu, Yang
Li, Jiawei
Zhang, Qihong
Li, Zejun
Zhao, Kai
author_facet Xu, Shangen
Yu, Lu
Teng, Qiaoyang
Li, Xuesong
Jin, Zheng
Qu, Yang
Li, Jiawei
Zhang, Qihong
Li, Zejun
Zhao, Kai
author_sort Xu, Shangen
collection PubMed
description DNA vaccination has great potential to treat or prevent avian influenza pandemics, but the technique may be limited by low immunogenicity and gene delivery in clinical testing. Here, to improve the immune efficacy of DNA vaccines against avian influenza, we prepared and tested the immunogenicity of 4 recombinant DNA vaccines containing 2 or 3 AIV antigens. The results revealed that chickens and mice immunized with plasmid DNA containing 3 antigens (HA gene from H9N2, and NA and HA genes from H5N1) exhibited a robust immune response than chickens and mice immunized with plasmid DNAs containing 2 antigenic genes. Subsequently, this study used pβH9N1SH5 as a model antigen to study the effect of dendritic polylysine (DGL) nanoparticles as a gene delivery system and adjuvant on antigen-specific immunity in mice models. At a ratio of 1:3 DGL/pβH9N1SH5 (w/w), the pβH9N1SH5/DGL NPs showed excellent physical and chemical properties, induced higher levels of HI antibodies, and larger CD3+/CD4+ T lymphocyte and CD3+/CD8+ T lymphocyte population, as well as the production of cytokines, namely, interferon (IFN)-γ, interleukin (IL)-2 compared with the naked pβH9N1SH5. Therefore, multiantigen gene expression methods using DGL as a delivery system may have broad application prospects in gene therapy.
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spelling pubmed-104281212023-08-17 Enhance immune response to H9 AIV DNA vaccine based on polygene expression and DGL nanoparticle encapsulation Xu, Shangen Yu, Lu Teng, Qiaoyang Li, Xuesong Jin, Zheng Qu, Yang Li, Jiawei Zhang, Qihong Li, Zejun Zhao, Kai Poult Sci IMMUNOLOGY, HEALTH AND DISEASE DNA vaccination has great potential to treat or prevent avian influenza pandemics, but the technique may be limited by low immunogenicity and gene delivery in clinical testing. Here, to improve the immune efficacy of DNA vaccines against avian influenza, we prepared and tested the immunogenicity of 4 recombinant DNA vaccines containing 2 or 3 AIV antigens. The results revealed that chickens and mice immunized with plasmid DNA containing 3 antigens (HA gene from H9N2, and NA and HA genes from H5N1) exhibited a robust immune response than chickens and mice immunized with plasmid DNAs containing 2 antigenic genes. Subsequently, this study used pβH9N1SH5 as a model antigen to study the effect of dendritic polylysine (DGL) nanoparticles as a gene delivery system and adjuvant on antigen-specific immunity in mice models. At a ratio of 1:3 DGL/pβH9N1SH5 (w/w), the pβH9N1SH5/DGL NPs showed excellent physical and chemical properties, induced higher levels of HI antibodies, and larger CD3+/CD4+ T lymphocyte and CD3+/CD8+ T lymphocyte population, as well as the production of cytokines, namely, interferon (IFN)-γ, interleukin (IL)-2 compared with the naked pβH9N1SH5. Therefore, multiantigen gene expression methods using DGL as a delivery system may have broad application prospects in gene therapy. Elsevier 2023-07-06 /pmc/articles/PMC10428121/ /pubmed/37542938 http://dx.doi.org/10.1016/j.psj.2023.102925 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle IMMUNOLOGY, HEALTH AND DISEASE
Xu, Shangen
Yu, Lu
Teng, Qiaoyang
Li, Xuesong
Jin, Zheng
Qu, Yang
Li, Jiawei
Zhang, Qihong
Li, Zejun
Zhao, Kai
Enhance immune response to H9 AIV DNA vaccine based on polygene expression and DGL nanoparticle encapsulation
title Enhance immune response to H9 AIV DNA vaccine based on polygene expression and DGL nanoparticle encapsulation
title_full Enhance immune response to H9 AIV DNA vaccine based on polygene expression and DGL nanoparticle encapsulation
title_fullStr Enhance immune response to H9 AIV DNA vaccine based on polygene expression and DGL nanoparticle encapsulation
title_full_unstemmed Enhance immune response to H9 AIV DNA vaccine based on polygene expression and DGL nanoparticle encapsulation
title_short Enhance immune response to H9 AIV DNA vaccine based on polygene expression and DGL nanoparticle encapsulation
title_sort enhance immune response to h9 aiv dna vaccine based on polygene expression and dgl nanoparticle encapsulation
topic IMMUNOLOGY, HEALTH AND DISEASE
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10428121/
https://www.ncbi.nlm.nih.gov/pubmed/37542938
http://dx.doi.org/10.1016/j.psj.2023.102925
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