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Simultaneous Salmonella and bacteriophage isolation on Modified Semisolid Rappaport Vassiliadis media
Salmonella represents a food safety concern worldwide. Despite the application of National Control Programs (NCP) against Salmonella, regulated by the European Union, every year the European Food Safety Authority reports new cases. On the look for new alternatives to antibiotics, bacteriophages, or...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10429287/ https://www.ncbi.nlm.nih.gov/pubmed/37579648 http://dx.doi.org/10.1016/j.psj.2023.102960 |
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author | Torres-Boncompte, Jan Catalá-Gregori, Pablo Garcia-Llorens, Josep Soriano, José M. Sevilla-Navarro, Sandra |
author_facet | Torres-Boncompte, Jan Catalá-Gregori, Pablo Garcia-Llorens, Josep Soriano, José M. Sevilla-Navarro, Sandra |
author_sort | Torres-Boncompte, Jan |
collection | PubMed |
description | Salmonella represents a food safety concern worldwide. Despite the application of National Control Programs (NCP) against Salmonella, regulated by the European Union, every year the European Food Safety Authority reports new cases. On the look for new alternatives to antibiotics, bacteriophages, or phages, rise as a promising alternative to treat multidrug resistance infections. Although they are known to be ubiquitous in the environment, their high specificity to host cells hinders their isolation and usage for phage therapy. The ISO 6579-1:2017 is performed as a reference method in the NCP and uses an unspecific media to enrich the sample the same way most phage isolation protocols do. Later, the protocol uses a more selective media to isolate the Salmonella, Modified Semisolid Rappaport Vassiliadis (MSRV). This paper aims to find out whether, due to the similarity between phage isolation protocols and the ISO 6579-1:2017, this last one could be used as a protocol to also isolate phages against the same bacterium that is being simultaneously isolated. To do so, 2 experiments were performed to assess phage isolation from MSRV media in in-vivo conditions. The results from experiments 1 and 2 proved that the MSRV media was usable for simultaneous phage and pathogen isolation through a single procedure. Additionally, there is a correlation between the antigenic formulae from the bacteria and the phage's host range, seeming to be effective against bacteria with similar antigenic formulae. |
format | Online Article Text |
id | pubmed-10429287 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-104292872023-08-17 Simultaneous Salmonella and bacteriophage isolation on Modified Semisolid Rappaport Vassiliadis media Torres-Boncompte, Jan Catalá-Gregori, Pablo Garcia-Llorens, Josep Soriano, José M. Sevilla-Navarro, Sandra Poult Sci MICROBIOLOGY AND FOOD SAFETY Salmonella represents a food safety concern worldwide. Despite the application of National Control Programs (NCP) against Salmonella, regulated by the European Union, every year the European Food Safety Authority reports new cases. On the look for new alternatives to antibiotics, bacteriophages, or phages, rise as a promising alternative to treat multidrug resistance infections. Although they are known to be ubiquitous in the environment, their high specificity to host cells hinders their isolation and usage for phage therapy. The ISO 6579-1:2017 is performed as a reference method in the NCP and uses an unspecific media to enrich the sample the same way most phage isolation protocols do. Later, the protocol uses a more selective media to isolate the Salmonella, Modified Semisolid Rappaport Vassiliadis (MSRV). This paper aims to find out whether, due to the similarity between phage isolation protocols and the ISO 6579-1:2017, this last one could be used as a protocol to also isolate phages against the same bacterium that is being simultaneously isolated. To do so, 2 experiments were performed to assess phage isolation from MSRV media in in-vivo conditions. The results from experiments 1 and 2 proved that the MSRV media was usable for simultaneous phage and pathogen isolation through a single procedure. Additionally, there is a correlation between the antigenic formulae from the bacteria and the phage's host range, seeming to be effective against bacteria with similar antigenic formulae. Elsevier 2023-07-25 /pmc/articles/PMC10429287/ /pubmed/37579648 http://dx.doi.org/10.1016/j.psj.2023.102960 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | MICROBIOLOGY AND FOOD SAFETY Torres-Boncompte, Jan Catalá-Gregori, Pablo Garcia-Llorens, Josep Soriano, José M. Sevilla-Navarro, Sandra Simultaneous Salmonella and bacteriophage isolation on Modified Semisolid Rappaport Vassiliadis media |
title | Simultaneous Salmonella and bacteriophage isolation on Modified Semisolid Rappaport Vassiliadis media |
title_full | Simultaneous Salmonella and bacteriophage isolation on Modified Semisolid Rappaport Vassiliadis media |
title_fullStr | Simultaneous Salmonella and bacteriophage isolation on Modified Semisolid Rappaport Vassiliadis media |
title_full_unstemmed | Simultaneous Salmonella and bacteriophage isolation on Modified Semisolid Rappaport Vassiliadis media |
title_short | Simultaneous Salmonella and bacteriophage isolation on Modified Semisolid Rappaport Vassiliadis media |
title_sort | simultaneous salmonella and bacteriophage isolation on modified semisolid rappaport vassiliadis media |
topic | MICROBIOLOGY AND FOOD SAFETY |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10429287/ https://www.ncbi.nlm.nih.gov/pubmed/37579648 http://dx.doi.org/10.1016/j.psj.2023.102960 |
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