Cargando…

Indigenous cattle of Sri Lanka: Genetic and phylogeographic relationship with Zebu of Indus Valley and South Indian origin

The present study reports the population structure, genetic admixture and phylogeography of cattle breeds of Sri Lanka viz. Batu Harak, Thawalam and White cattle. Moderately high level of genetic diversity was observed in all the three Sri Lankan zebu cattle breeds. Estimates of inbreeding for Thawa...

Descripción completa

Detalles Bibliográficos
Autores principales: L. G. S., Lokugalappatti, Wickramasinghe, Saumya, P. A. B. D., Alexander, Abbas, Kamran, Hussain, Tanveer, Ramasamy, Saravanan, Manomohan, Vandana, Tapsoba, Arnaud Stephane R., Pichler, Rudolf, Babar, Masroor E., Periasamy, Kathiravan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10431622/
https://www.ncbi.nlm.nih.gov/pubmed/37585485
http://dx.doi.org/10.1371/journal.pone.0282761
_version_ 1785091245332234240
author L. G. S., Lokugalappatti
Wickramasinghe, Saumya
P. A. B. D., Alexander
Abbas, Kamran
Hussain, Tanveer
Ramasamy, Saravanan
Manomohan, Vandana
Tapsoba, Arnaud Stephane R.
Pichler, Rudolf
Babar, Masroor E.
Periasamy, Kathiravan
author_facet L. G. S., Lokugalappatti
Wickramasinghe, Saumya
P. A. B. D., Alexander
Abbas, Kamran
Hussain, Tanveer
Ramasamy, Saravanan
Manomohan, Vandana
Tapsoba, Arnaud Stephane R.
Pichler, Rudolf
Babar, Masroor E.
Periasamy, Kathiravan
author_sort L. G. S., Lokugalappatti
collection PubMed
description The present study reports the population structure, genetic admixture and phylogeography of cattle breeds of Sri Lanka viz. Batu Harak, Thawalam and White cattle. Moderately high level of genetic diversity was observed in all the three Sri Lankan zebu cattle breeds. Estimates of inbreeding for Thawalam and White cattle breeds were relatively high with 6.1% and 7.2% respectively. Genetic differentiation of Sri Lankan Zebu (Batu Harak and White cattle) was lowest with Red Sindhi among Indus Valley Zebu while it was lowest with Hallikar among the South Indian cattle. Global F statistics showed 6.5% differences among all the investigated Zebu cattle breeds and 1.9% differences among Sri Lankan Zebu breeds. The Sri Lankan Zebu cattle breeds showed strong genetic relationships with Hallikar cattle, an ancient breed considered to be ancestor for most of the Mysore type draught cattle breeds of South India. Genetic admixture analysis revealed high levels of breed purity in Lanka White cattle with >97% Zebu ancestry. However, significant taurine admixture was observed in Batu Harak and Thawalam cattle. Two major Zebu haplogroups, I1 and I2 were observed in Sri Lankan Zebu with the former predominating the later in all the three breeds. A total of 112 haplotypes were observed in the studied breeds, of which 50 haplotypes were found in Sri Lankan Zebu cattle. Mismatch analysis revealed unimodal distribution in all the three breeds indicating population expansion. The sum of squared deviations (SSD) and raggedness index were non-significant in both the lineages of all the three breeds except for I1 lineage of Thawalam cattle (P<0.01) and I2 lineage of Batu Harak cattle (P<0.05). The results of neutrality tests revealed negative Tajima’s D values for both the lineages of Batu Harak (P>0.05) and White cattle (P>0.05) indicating an excess of low frequency polymorphisms and demographic expansion. Genetic dilution of native Zebu cattle germplasm observed in the study is a cause for concern. Hence, it is imperative that national breeding organizations consider establishing conservation units for the three native cattle breeds to maintain breed purity and initiate genetic improvement programs.
format Online
Article
Text
id pubmed-10431622
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-104316222023-08-17 Indigenous cattle of Sri Lanka: Genetic and phylogeographic relationship with Zebu of Indus Valley and South Indian origin L. G. S., Lokugalappatti Wickramasinghe, Saumya P. A. B. D., Alexander Abbas, Kamran Hussain, Tanveer Ramasamy, Saravanan Manomohan, Vandana Tapsoba, Arnaud Stephane R. Pichler, Rudolf Babar, Masroor E. Periasamy, Kathiravan PLoS One Research Article The present study reports the population structure, genetic admixture and phylogeography of cattle breeds of Sri Lanka viz. Batu Harak, Thawalam and White cattle. Moderately high level of genetic diversity was observed in all the three Sri Lankan zebu cattle breeds. Estimates of inbreeding for Thawalam and White cattle breeds were relatively high with 6.1% and 7.2% respectively. Genetic differentiation of Sri Lankan Zebu (Batu Harak and White cattle) was lowest with Red Sindhi among Indus Valley Zebu while it was lowest with Hallikar among the South Indian cattle. Global F statistics showed 6.5% differences among all the investigated Zebu cattle breeds and 1.9% differences among Sri Lankan Zebu breeds. The Sri Lankan Zebu cattle breeds showed strong genetic relationships with Hallikar cattle, an ancient breed considered to be ancestor for most of the Mysore type draught cattle breeds of South India. Genetic admixture analysis revealed high levels of breed purity in Lanka White cattle with >97% Zebu ancestry. However, significant taurine admixture was observed in Batu Harak and Thawalam cattle. Two major Zebu haplogroups, I1 and I2 were observed in Sri Lankan Zebu with the former predominating the later in all the three breeds. A total of 112 haplotypes were observed in the studied breeds, of which 50 haplotypes were found in Sri Lankan Zebu cattle. Mismatch analysis revealed unimodal distribution in all the three breeds indicating population expansion. The sum of squared deviations (SSD) and raggedness index were non-significant in both the lineages of all the three breeds except for I1 lineage of Thawalam cattle (P<0.01) and I2 lineage of Batu Harak cattle (P<0.05). The results of neutrality tests revealed negative Tajima’s D values for both the lineages of Batu Harak (P>0.05) and White cattle (P>0.05) indicating an excess of low frequency polymorphisms and demographic expansion. Genetic dilution of native Zebu cattle germplasm observed in the study is a cause for concern. Hence, it is imperative that national breeding organizations consider establishing conservation units for the three native cattle breeds to maintain breed purity and initiate genetic improvement programs. Public Library of Science 2023-08-16 /pmc/articles/PMC10431622/ /pubmed/37585485 http://dx.doi.org/10.1371/journal.pone.0282761 Text en © 2023 L. G. S. et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
L. G. S., Lokugalappatti
Wickramasinghe, Saumya
P. A. B. D., Alexander
Abbas, Kamran
Hussain, Tanveer
Ramasamy, Saravanan
Manomohan, Vandana
Tapsoba, Arnaud Stephane R.
Pichler, Rudolf
Babar, Masroor E.
Periasamy, Kathiravan
Indigenous cattle of Sri Lanka: Genetic and phylogeographic relationship with Zebu of Indus Valley and South Indian origin
title Indigenous cattle of Sri Lanka: Genetic and phylogeographic relationship with Zebu of Indus Valley and South Indian origin
title_full Indigenous cattle of Sri Lanka: Genetic and phylogeographic relationship with Zebu of Indus Valley and South Indian origin
title_fullStr Indigenous cattle of Sri Lanka: Genetic and phylogeographic relationship with Zebu of Indus Valley and South Indian origin
title_full_unstemmed Indigenous cattle of Sri Lanka: Genetic and phylogeographic relationship with Zebu of Indus Valley and South Indian origin
title_short Indigenous cattle of Sri Lanka: Genetic and phylogeographic relationship with Zebu of Indus Valley and South Indian origin
title_sort indigenous cattle of sri lanka: genetic and phylogeographic relationship with zebu of indus valley and south indian origin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10431622/
https://www.ncbi.nlm.nih.gov/pubmed/37585485
http://dx.doi.org/10.1371/journal.pone.0282761
work_keys_str_mv AT lgslokugalappatti indigenouscattleofsrilankageneticandphylogeographicrelationshipwithzebuofindusvalleyandsouthindianorigin
AT wickramasinghesaumya indigenouscattleofsrilankageneticandphylogeographicrelationshipwithzebuofindusvalleyandsouthindianorigin
AT pabdalexander indigenouscattleofsrilankageneticandphylogeographicrelationshipwithzebuofindusvalleyandsouthindianorigin
AT abbaskamran indigenouscattleofsrilankageneticandphylogeographicrelationshipwithzebuofindusvalleyandsouthindianorigin
AT hussaintanveer indigenouscattleofsrilankageneticandphylogeographicrelationshipwithzebuofindusvalleyandsouthindianorigin
AT ramasamysaravanan indigenouscattleofsrilankageneticandphylogeographicrelationshipwithzebuofindusvalleyandsouthindianorigin
AT manomohanvandana indigenouscattleofsrilankageneticandphylogeographicrelationshipwithzebuofindusvalleyandsouthindianorigin
AT tapsobaarnaudstephaner indigenouscattleofsrilankageneticandphylogeographicrelationshipwithzebuofindusvalleyandsouthindianorigin
AT pichlerrudolf indigenouscattleofsrilankageneticandphylogeographicrelationshipwithzebuofindusvalleyandsouthindianorigin
AT babarmasroore indigenouscattleofsrilankageneticandphylogeographicrelationshipwithzebuofindusvalleyandsouthindianorigin
AT periasamykathiravan indigenouscattleofsrilankageneticandphylogeographicrelationshipwithzebuofindusvalleyandsouthindianorigin