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Emergence of linkage between cooperative RNA replicators encoding replication and metabolic enzymes through experimental evolution
The integration of individually replicating genes into a primitive chromosome is a key evolutionary transition in the development of life, allowing the simultaneous inheritance of genes. However, how this transition occurred is unclear because the extended size of primitive chromosomes replicate slo...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10431678/ https://www.ncbi.nlm.nih.gov/pubmed/37540715 http://dx.doi.org/10.1371/journal.pgen.1010471 |
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author | Ueda, Kensuke Mizuuchi, Ryo Ichihashi, Norikazu |
author_facet | Ueda, Kensuke Mizuuchi, Ryo Ichihashi, Norikazu |
author_sort | Ueda, Kensuke |
collection | PubMed |
description | The integration of individually replicating genes into a primitive chromosome is a key evolutionary transition in the development of life, allowing the simultaneous inheritance of genes. However, how this transition occurred is unclear because the extended size of primitive chromosomes replicate slower than unlinked genes. Theoretical studies have suggested that a primitive chromosome can evolve in the presence of cell-like compartments, as the physical linkage prevents the stochastic loss of essential genes upon division, but experimental support for this is lacking. Here, we demonstrate the evolution of a chromosome-like RNA from two cooperative RNA replicators encoding replication and metabolic enzymes. Through their long-term replication in cell-like compartments, linked RNAs emerged with the two cooperative RNAs connected end-to-end. The linked RNAs had different mutation patterns than the two unlinked RNAs, suggesting that they were maintained as partially distinct lineages in the population. Our results provide experimental evidence supporting the plausibility of the evolution of a primitive chromosome from unlinked gene fragments, an important step in the emergence of complex biological systems. |
format | Online Article Text |
id | pubmed-10431678 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-104316782023-08-17 Emergence of linkage between cooperative RNA replicators encoding replication and metabolic enzymes through experimental evolution Ueda, Kensuke Mizuuchi, Ryo Ichihashi, Norikazu PLoS Genet Research Article The integration of individually replicating genes into a primitive chromosome is a key evolutionary transition in the development of life, allowing the simultaneous inheritance of genes. However, how this transition occurred is unclear because the extended size of primitive chromosomes replicate slower than unlinked genes. Theoretical studies have suggested that a primitive chromosome can evolve in the presence of cell-like compartments, as the physical linkage prevents the stochastic loss of essential genes upon division, but experimental support for this is lacking. Here, we demonstrate the evolution of a chromosome-like RNA from two cooperative RNA replicators encoding replication and metabolic enzymes. Through their long-term replication in cell-like compartments, linked RNAs emerged with the two cooperative RNAs connected end-to-end. The linked RNAs had different mutation patterns than the two unlinked RNAs, suggesting that they were maintained as partially distinct lineages in the population. Our results provide experimental evidence supporting the plausibility of the evolution of a primitive chromosome from unlinked gene fragments, an important step in the emergence of complex biological systems. Public Library of Science 2023-08-04 /pmc/articles/PMC10431678/ /pubmed/37540715 http://dx.doi.org/10.1371/journal.pgen.1010471 Text en © 2023 Ueda et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Ueda, Kensuke Mizuuchi, Ryo Ichihashi, Norikazu Emergence of linkage between cooperative RNA replicators encoding replication and metabolic enzymes through experimental evolution |
title | Emergence of linkage between cooperative RNA replicators encoding replication and metabolic enzymes through experimental evolution |
title_full | Emergence of linkage between cooperative RNA replicators encoding replication and metabolic enzymes through experimental evolution |
title_fullStr | Emergence of linkage between cooperative RNA replicators encoding replication and metabolic enzymes through experimental evolution |
title_full_unstemmed | Emergence of linkage between cooperative RNA replicators encoding replication and metabolic enzymes through experimental evolution |
title_short | Emergence of linkage between cooperative RNA replicators encoding replication and metabolic enzymes through experimental evolution |
title_sort | emergence of linkage between cooperative rna replicators encoding replication and metabolic enzymes through experimental evolution |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10431678/ https://www.ncbi.nlm.nih.gov/pubmed/37540715 http://dx.doi.org/10.1371/journal.pgen.1010471 |
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