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Substrate type and CO(2) addition significantly influence succinic acid production of Basfia succiniciproducens
Metabolic engineering has shown that optimizing metabolic pathways’ fluxes for industrial purposes requires a methodical approach. Accordingly, in this study, in silico metabolic modeling was utilized to characterize the lesser-known strain Basfia succiniciproducens under different environmental con...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10432361/ https://www.ncbi.nlm.nih.gov/pubmed/37395870 http://dx.doi.org/10.1007/s10529-023-03406-7 |
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author | Balázs, Márta Bartos, Hunor Lányi, Szabolcs Bodor, Zsolt Miklóssy, Ildikó |
author_facet | Balázs, Márta Bartos, Hunor Lányi, Szabolcs Bodor, Zsolt Miklóssy, Ildikó |
author_sort | Balázs, Márta |
collection | PubMed |
description | Metabolic engineering has shown that optimizing metabolic pathways’ fluxes for industrial purposes requires a methodical approach. Accordingly, in this study, in silico metabolic modeling was utilized to characterize the lesser-known strain Basfia succiniciproducens under different environmental conditions, followed by the use of industrially relevant substrates for succinic acid synthesis. Based on RT-qPCR carried out in flask experiments, we discovered a relatively large difference in the expression levels of ldhA gene compared to glucose in both xylose and glycerol cultures. In bioreactor-scale fermentations, the impact of different gas phases (CO(2), CO(2)/AIR) on biomass yield, substrate consumption, and metabolite profiles was also investigated. In the case of glycerol, the addition of CO(2) increased biomass as well as target product formation, while using CO(2)/AIR gas phase resulted in higher target product yield (0.184 mM⋅mM(−1)). In case of xylose, using CO(2) alone would result in higher succinic acid production (0.277 mM⋅mM(−1)). The promising rumen bacteria, B. succiniciproducens, has shown to be suitable for succinic acid production from both xylose and glycerol. As a result, our findings present new opportunities for broadening the range of raw materials used in this significant biochemical process. Our study also sheds light on fermentation parameter optimization for this strain, namely that, CO(2)/AIR supply has a positive effect on target product formation. |
format | Online Article Text |
id | pubmed-10432361 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-104323612023-08-18 Substrate type and CO(2) addition significantly influence succinic acid production of Basfia succiniciproducens Balázs, Márta Bartos, Hunor Lányi, Szabolcs Bodor, Zsolt Miklóssy, Ildikó Biotechnol Lett Original Research Paper Metabolic engineering has shown that optimizing metabolic pathways’ fluxes for industrial purposes requires a methodical approach. Accordingly, in this study, in silico metabolic modeling was utilized to characterize the lesser-known strain Basfia succiniciproducens under different environmental conditions, followed by the use of industrially relevant substrates for succinic acid synthesis. Based on RT-qPCR carried out in flask experiments, we discovered a relatively large difference in the expression levels of ldhA gene compared to glucose in both xylose and glycerol cultures. In bioreactor-scale fermentations, the impact of different gas phases (CO(2), CO(2)/AIR) on biomass yield, substrate consumption, and metabolite profiles was also investigated. In the case of glycerol, the addition of CO(2) increased biomass as well as target product formation, while using CO(2)/AIR gas phase resulted in higher target product yield (0.184 mM⋅mM(−1)). In case of xylose, using CO(2) alone would result in higher succinic acid production (0.277 mM⋅mM(−1)). The promising rumen bacteria, B. succiniciproducens, has shown to be suitable for succinic acid production from both xylose and glycerol. As a result, our findings present new opportunities for broadening the range of raw materials used in this significant biochemical process. Our study also sheds light on fermentation parameter optimization for this strain, namely that, CO(2)/AIR supply has a positive effect on target product formation. Springer Netherlands 2023-07-03 2023 /pmc/articles/PMC10432361/ /pubmed/37395870 http://dx.doi.org/10.1007/s10529-023-03406-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Research Paper Balázs, Márta Bartos, Hunor Lányi, Szabolcs Bodor, Zsolt Miklóssy, Ildikó Substrate type and CO(2) addition significantly influence succinic acid production of Basfia succiniciproducens |
title | Substrate type and CO(2) addition significantly influence succinic acid production of Basfia succiniciproducens |
title_full | Substrate type and CO(2) addition significantly influence succinic acid production of Basfia succiniciproducens |
title_fullStr | Substrate type and CO(2) addition significantly influence succinic acid production of Basfia succiniciproducens |
title_full_unstemmed | Substrate type and CO(2) addition significantly influence succinic acid production of Basfia succiniciproducens |
title_short | Substrate type and CO(2) addition significantly influence succinic acid production of Basfia succiniciproducens |
title_sort | substrate type and co(2) addition significantly influence succinic acid production of basfia succiniciproducens |
topic | Original Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10432361/ https://www.ncbi.nlm.nih.gov/pubmed/37395870 http://dx.doi.org/10.1007/s10529-023-03406-7 |
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