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Protocol for detection of ferroptosis in cultured cells

Mammalian cells can die by apoptosis or by one of several non-apoptotic mechanisms, such as ferroptosis. Here, we present a protocol to distinguish ferroptosis from other cell death mechanisms in cultured cells. We describe steps for seeding cells, administering mechanism-specific cell death inducer...

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Detalles Bibliográficos
Autores principales: Murray, Magdalena B., Leak, Logan B., Lee, Weaverly Colleen, Dixon, Scott J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10432795/
https://www.ncbi.nlm.nih.gov/pubmed/37556320
http://dx.doi.org/10.1016/j.xpro.2023.102457
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author Murray, Magdalena B.
Leak, Logan B.
Lee, Weaverly Colleen
Dixon, Scott J.
author_facet Murray, Magdalena B.
Leak, Logan B.
Lee, Weaverly Colleen
Dixon, Scott J.
author_sort Murray, Magdalena B.
collection PubMed
description Mammalian cells can die by apoptosis or by one of several non-apoptotic mechanisms, such as ferroptosis. Here, we present a protocol to distinguish ferroptosis from other cell death mechanisms in cultured cells. We describe steps for seeding cells, administering mechanism-specific cell death inducers and inhibitors, and measuring cell death and viability. We then detail the use of molecular markers to verify mechanisms of cell death. This protocol can be used to identify and distinguish ferroptosis in 2D and 3D cultures. For complete details on the use and execution of this protocol, please refer to Ko, et al. (2019),(1) Magtanong, et al. (2022),(2) and Armenta, et al. (2022).(3)
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spelling pubmed-104327952023-08-18 Protocol for detection of ferroptosis in cultured cells Murray, Magdalena B. Leak, Logan B. Lee, Weaverly Colleen Dixon, Scott J. STAR Protoc Protocol Mammalian cells can die by apoptosis or by one of several non-apoptotic mechanisms, such as ferroptosis. Here, we present a protocol to distinguish ferroptosis from other cell death mechanisms in cultured cells. We describe steps for seeding cells, administering mechanism-specific cell death inducers and inhibitors, and measuring cell death and viability. We then detail the use of molecular markers to verify mechanisms of cell death. This protocol can be used to identify and distinguish ferroptosis in 2D and 3D cultures. For complete details on the use and execution of this protocol, please refer to Ko, et al. (2019),(1) Magtanong, et al. (2022),(2) and Armenta, et al. (2022).(3) Elsevier 2023-08-08 /pmc/articles/PMC10432795/ /pubmed/37556320 http://dx.doi.org/10.1016/j.xpro.2023.102457 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Murray, Magdalena B.
Leak, Logan B.
Lee, Weaverly Colleen
Dixon, Scott J.
Protocol for detection of ferroptosis in cultured cells
title Protocol for detection of ferroptosis in cultured cells
title_full Protocol for detection of ferroptosis in cultured cells
title_fullStr Protocol for detection of ferroptosis in cultured cells
title_full_unstemmed Protocol for detection of ferroptosis in cultured cells
title_short Protocol for detection of ferroptosis in cultured cells
title_sort protocol for detection of ferroptosis in cultured cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10432795/
https://www.ncbi.nlm.nih.gov/pubmed/37556320
http://dx.doi.org/10.1016/j.xpro.2023.102457
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