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Protocol for detection of ferroptosis in cultured cells
Mammalian cells can die by apoptosis or by one of several non-apoptotic mechanisms, such as ferroptosis. Here, we present a protocol to distinguish ferroptosis from other cell death mechanisms in cultured cells. We describe steps for seeding cells, administering mechanism-specific cell death inducer...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10432795/ https://www.ncbi.nlm.nih.gov/pubmed/37556320 http://dx.doi.org/10.1016/j.xpro.2023.102457 |
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author | Murray, Magdalena B. Leak, Logan B. Lee, Weaverly Colleen Dixon, Scott J. |
author_facet | Murray, Magdalena B. Leak, Logan B. Lee, Weaverly Colleen Dixon, Scott J. |
author_sort | Murray, Magdalena B. |
collection | PubMed |
description | Mammalian cells can die by apoptosis or by one of several non-apoptotic mechanisms, such as ferroptosis. Here, we present a protocol to distinguish ferroptosis from other cell death mechanisms in cultured cells. We describe steps for seeding cells, administering mechanism-specific cell death inducers and inhibitors, and measuring cell death and viability. We then detail the use of molecular markers to verify mechanisms of cell death. This protocol can be used to identify and distinguish ferroptosis in 2D and 3D cultures. For complete details on the use and execution of this protocol, please refer to Ko, et al. (2019),(1) Magtanong, et al. (2022),(2) and Armenta, et al. (2022).(3) |
format | Online Article Text |
id | pubmed-10432795 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-104327952023-08-18 Protocol for detection of ferroptosis in cultured cells Murray, Magdalena B. Leak, Logan B. Lee, Weaverly Colleen Dixon, Scott J. STAR Protoc Protocol Mammalian cells can die by apoptosis or by one of several non-apoptotic mechanisms, such as ferroptosis. Here, we present a protocol to distinguish ferroptosis from other cell death mechanisms in cultured cells. We describe steps for seeding cells, administering mechanism-specific cell death inducers and inhibitors, and measuring cell death and viability. We then detail the use of molecular markers to verify mechanisms of cell death. This protocol can be used to identify and distinguish ferroptosis in 2D and 3D cultures. For complete details on the use and execution of this protocol, please refer to Ko, et al. (2019),(1) Magtanong, et al. (2022),(2) and Armenta, et al. (2022).(3) Elsevier 2023-08-08 /pmc/articles/PMC10432795/ /pubmed/37556320 http://dx.doi.org/10.1016/j.xpro.2023.102457 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Murray, Magdalena B. Leak, Logan B. Lee, Weaverly Colleen Dixon, Scott J. Protocol for detection of ferroptosis in cultured cells |
title | Protocol for detection of ferroptosis in cultured cells |
title_full | Protocol for detection of ferroptosis in cultured cells |
title_fullStr | Protocol for detection of ferroptosis in cultured cells |
title_full_unstemmed | Protocol for detection of ferroptosis in cultured cells |
title_short | Protocol for detection of ferroptosis in cultured cells |
title_sort | protocol for detection of ferroptosis in cultured cells |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10432795/ https://www.ncbi.nlm.nih.gov/pubmed/37556320 http://dx.doi.org/10.1016/j.xpro.2023.102457 |
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