The large extracellular loop is important for recruiting CD63 to exosomes

Exosomes are small extracellular vesicles that are secreted from cells. To characterize exosome fraction marker proteins of the tetraspanin family in particular, CD9, CD63, and CD81 are routinely used. CD63 expression constructs were employed to investigate the influence of the large extracellular l...

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Detalles Bibliográficos
Autores principales: Ivanusic, Daniel, Denner, Joachim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10432940/
https://www.ncbi.nlm.nih.gov/pubmed/37602284
http://dx.doi.org/10.17912/micropub.biology.000842
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author Ivanusic, Daniel
Denner, Joachim
author_facet Ivanusic, Daniel
Denner, Joachim
author_sort Ivanusic, Daniel
collection PubMed
description Exosomes are small extracellular vesicles that are secreted from cells. To characterize exosome fraction marker proteins of the tetraspanin family in particular, CD9, CD63, and CD81 are routinely used. CD63 expression constructs were employed to investigate the influence of the large extracellular loop (LEL) of CD63 on sorting into exosomes. When the LEL of CD63 fused with mCherry was deleted, the protein was no longer found in the purified exosome fraction. This finding demonstrates the importance of the LEL sequence for the recruitment of CD63 into exosomes.
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spelling pubmed-104329402023-08-18 The large extracellular loop is important for recruiting CD63 to exosomes Ivanusic, Daniel Denner, Joachim MicroPubl Biol New Finding Exosomes are small extracellular vesicles that are secreted from cells. To characterize exosome fraction marker proteins of the tetraspanin family in particular, CD9, CD63, and CD81 are routinely used. CD63 expression constructs were employed to investigate the influence of the large extracellular loop (LEL) of CD63 on sorting into exosomes. When the LEL of CD63 fused with mCherry was deleted, the protein was no longer found in the purified exosome fraction. This finding demonstrates the importance of the LEL sequence for the recruitment of CD63 into exosomes. Caltech Library 2023-08-03 /pmc/articles/PMC10432940/ /pubmed/37602284 http://dx.doi.org/10.17912/micropub.biology.000842 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Ivanusic, Daniel
Denner, Joachim
The large extracellular loop is important for recruiting CD63 to exosomes
title The large extracellular loop is important for recruiting CD63 to exosomes
title_full The large extracellular loop is important for recruiting CD63 to exosomes
title_fullStr The large extracellular loop is important for recruiting CD63 to exosomes
title_full_unstemmed The large extracellular loop is important for recruiting CD63 to exosomes
title_short The large extracellular loop is important for recruiting CD63 to exosomes
title_sort large extracellular loop is important for recruiting cd63 to exosomes
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10432940/
https://www.ncbi.nlm.nih.gov/pubmed/37602284
http://dx.doi.org/10.17912/micropub.biology.000842
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