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Assembly and analysis of the mitochondrial genome of Prunella vulgaris

Prunella vulgaris (Lamiaceae) is widely distributed in Eurasia. Former studies have demonstrated that P. vulgaris has a wide range of pharmacological effects. Nevertheless, no complete P. vulgaris mitochondrial genome has been reported, which limits further understanding of the biology of P. vulgari...

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Autores principales: Sun, Zhihao, Wu, Ya, Fan, Pengyu, Guo, Dengli, Zhang, Sanyin, Song, Chi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10433383/
https://www.ncbi.nlm.nih.gov/pubmed/37600185
http://dx.doi.org/10.3389/fpls.2023.1237822
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author Sun, Zhihao
Wu, Ya
Fan, Pengyu
Guo, Dengli
Zhang, Sanyin
Song, Chi
author_facet Sun, Zhihao
Wu, Ya
Fan, Pengyu
Guo, Dengli
Zhang, Sanyin
Song, Chi
author_sort Sun, Zhihao
collection PubMed
description Prunella vulgaris (Lamiaceae) is widely distributed in Eurasia. Former studies have demonstrated that P. vulgaris has a wide range of pharmacological effects. Nevertheless, no complete P. vulgaris mitochondrial genome has been reported, which limits further understanding of the biology of P. vulgaris. Here, we assembled the first complete mitochondrial genome of P. vulgaris using a hybrid assembly strategy based on sequencing data from both Nanopore and Illumina platforms. Then, the mitochondrial genome of P. vulgaris was analyzed comprehensively in terms of gene content, codon preference, intercellular gene transfer, phylogeny, and RNA editing. The mitochondrial genome of P. vulgaris has two circular structures. It has a total length of 297, 777 bp, a GC content of 43.92%, and 29 unique protein-coding genes (PCGs). There are 76 simple sequence repeats (SSRs) in the mitochondrial genome, of which tetrameric accounts for a large percentage (43.4%). A comparative analysis between the mitochondrial and chloroplast genomes revealed that 36 homologous fragments exist in them, with a total length of 28, 895 bp. The phylogenetic analysis showed that P. vulgaris belongs to the Lamiales family Lamiaceae and P. vulgaris is closely related to Salvia miltiorrhiza. In addition, the mitochondrial genome sequences of seven species of Lamiaceae are unconservative in their alignments and undergo frequent genome reorganization. This work reports for the first time the complete mitochondrial genome of P. vulgaris, which provides useful genetic information for further Prunella studies.
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spelling pubmed-104333832023-08-18 Assembly and analysis of the mitochondrial genome of Prunella vulgaris Sun, Zhihao Wu, Ya Fan, Pengyu Guo, Dengli Zhang, Sanyin Song, Chi Front Plant Sci Plant Science Prunella vulgaris (Lamiaceae) is widely distributed in Eurasia. Former studies have demonstrated that P. vulgaris has a wide range of pharmacological effects. Nevertheless, no complete P. vulgaris mitochondrial genome has been reported, which limits further understanding of the biology of P. vulgaris. Here, we assembled the first complete mitochondrial genome of P. vulgaris using a hybrid assembly strategy based on sequencing data from both Nanopore and Illumina platforms. Then, the mitochondrial genome of P. vulgaris was analyzed comprehensively in terms of gene content, codon preference, intercellular gene transfer, phylogeny, and RNA editing. The mitochondrial genome of P. vulgaris has two circular structures. It has a total length of 297, 777 bp, a GC content of 43.92%, and 29 unique protein-coding genes (PCGs). There are 76 simple sequence repeats (SSRs) in the mitochondrial genome, of which tetrameric accounts for a large percentage (43.4%). A comparative analysis between the mitochondrial and chloroplast genomes revealed that 36 homologous fragments exist in them, with a total length of 28, 895 bp. The phylogenetic analysis showed that P. vulgaris belongs to the Lamiales family Lamiaceae and P. vulgaris is closely related to Salvia miltiorrhiza. In addition, the mitochondrial genome sequences of seven species of Lamiaceae are unconservative in their alignments and undergo frequent genome reorganization. This work reports for the first time the complete mitochondrial genome of P. vulgaris, which provides useful genetic information for further Prunella studies. Frontiers Media S.A. 2023-08-02 /pmc/articles/PMC10433383/ /pubmed/37600185 http://dx.doi.org/10.3389/fpls.2023.1237822 Text en Copyright © 2023 Sun, Wu, Fan, Guo, Zhang and Song https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Sun, Zhihao
Wu, Ya
Fan, Pengyu
Guo, Dengli
Zhang, Sanyin
Song, Chi
Assembly and analysis of the mitochondrial genome of Prunella vulgaris
title Assembly and analysis of the mitochondrial genome of Prunella vulgaris
title_full Assembly and analysis of the mitochondrial genome of Prunella vulgaris
title_fullStr Assembly and analysis of the mitochondrial genome of Prunella vulgaris
title_full_unstemmed Assembly and analysis of the mitochondrial genome of Prunella vulgaris
title_short Assembly and analysis of the mitochondrial genome of Prunella vulgaris
title_sort assembly and analysis of the mitochondrial genome of prunella vulgaris
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10433383/
https://www.ncbi.nlm.nih.gov/pubmed/37600185
http://dx.doi.org/10.3389/fpls.2023.1237822
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