Fused expression of Sm1-Chit42 proteins for synergistic mycoparasitic response of Trichoderma afroharzianum on Botrytis cinerea

Sm1 and Chit42 of Trichoderma have been universally confirmed as crucial biocontrol factors against pathogen infection through induced resistance and mycoparasitism, respectively. However, not enough work has been conducted to understand the novel function of fused expression of these two proteins i...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Hongyi, Wang, Shaoqing, Lang, Bo, Li, Yaqian, Wang, Xinhua, Chen, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10433591/
https://www.ncbi.nlm.nih.gov/pubmed/37592265
http://dx.doi.org/10.1186/s12934-023-02151-w
_version_ 1785091682868396032
author Liu, Hongyi
Wang, Shaoqing
Lang, Bo
Li, Yaqian
Wang, Xinhua
Chen, Jie
author_facet Liu, Hongyi
Wang, Shaoqing
Lang, Bo
Li, Yaqian
Wang, Xinhua
Chen, Jie
author_sort Liu, Hongyi
collection PubMed
description Sm1 and Chit42 of Trichoderma have been universally confirmed as crucial biocontrol factors against pathogen infection through induced resistance and mycoparasitism, respectively. However, not enough work has been conducted to understand the novel function of fused expression of these two proteins in Trichoderma. The results of this study demonstrated that Sm1-Chit42 protein (SCf) engineered T. afroharzianum strain OE:SCf exerted synergistic inhibition to Botrytis cinerea growth at multiple stages of mycoparasitic interaction of T. afroharzianum and B. cinerea including chemotropism sensing, hyphal coiling, hydrophobicity modulation, cell wall adhesion, virulence reduction and pathogen killing by ROS. These results highlight a novel mycoparasitic system in Trichoderma strains engineered with Sm1-Chit42 chimeric protein to combat B. cinerea growth and reproduction, which would lay a strong foundation for exploring a new engineered Trichoderma biofungicide created with chimeric proteins in the future. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12934-023-02151-w.
format Online
Article
Text
id pubmed-10433591
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-104335912023-08-18 Fused expression of Sm1-Chit42 proteins for synergistic mycoparasitic response of Trichoderma afroharzianum on Botrytis cinerea Liu, Hongyi Wang, Shaoqing Lang, Bo Li, Yaqian Wang, Xinhua Chen, Jie Microb Cell Fact Research Sm1 and Chit42 of Trichoderma have been universally confirmed as crucial biocontrol factors against pathogen infection through induced resistance and mycoparasitism, respectively. However, not enough work has been conducted to understand the novel function of fused expression of these two proteins in Trichoderma. The results of this study demonstrated that Sm1-Chit42 protein (SCf) engineered T. afroharzianum strain OE:SCf exerted synergistic inhibition to Botrytis cinerea growth at multiple stages of mycoparasitic interaction of T. afroharzianum and B. cinerea including chemotropism sensing, hyphal coiling, hydrophobicity modulation, cell wall adhesion, virulence reduction and pathogen killing by ROS. These results highlight a novel mycoparasitic system in Trichoderma strains engineered with Sm1-Chit42 chimeric protein to combat B. cinerea growth and reproduction, which would lay a strong foundation for exploring a new engineered Trichoderma biofungicide created with chimeric proteins in the future. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12934-023-02151-w. BioMed Central 2023-08-17 /pmc/articles/PMC10433591/ /pubmed/37592265 http://dx.doi.org/10.1186/s12934-023-02151-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Liu, Hongyi
Wang, Shaoqing
Lang, Bo
Li, Yaqian
Wang, Xinhua
Chen, Jie
Fused expression of Sm1-Chit42 proteins for synergistic mycoparasitic response of Trichoderma afroharzianum on Botrytis cinerea
title Fused expression of Sm1-Chit42 proteins for synergistic mycoparasitic response of Trichoderma afroharzianum on Botrytis cinerea
title_full Fused expression of Sm1-Chit42 proteins for synergistic mycoparasitic response of Trichoderma afroharzianum on Botrytis cinerea
title_fullStr Fused expression of Sm1-Chit42 proteins for synergistic mycoparasitic response of Trichoderma afroharzianum on Botrytis cinerea
title_full_unstemmed Fused expression of Sm1-Chit42 proteins for synergistic mycoparasitic response of Trichoderma afroharzianum on Botrytis cinerea
title_short Fused expression of Sm1-Chit42 proteins for synergistic mycoparasitic response of Trichoderma afroharzianum on Botrytis cinerea
title_sort fused expression of sm1-chit42 proteins for synergistic mycoparasitic response of trichoderma afroharzianum on botrytis cinerea
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10433591/
https://www.ncbi.nlm.nih.gov/pubmed/37592265
http://dx.doi.org/10.1186/s12934-023-02151-w
work_keys_str_mv AT liuhongyi fusedexpressionofsm1chit42proteinsforsynergisticmycoparasiticresponseoftrichodermaafroharzianumonbotrytiscinerea
AT wangshaoqing fusedexpressionofsm1chit42proteinsforsynergisticmycoparasiticresponseoftrichodermaafroharzianumonbotrytiscinerea
AT langbo fusedexpressionofsm1chit42proteinsforsynergisticmycoparasiticresponseoftrichodermaafroharzianumonbotrytiscinerea
AT liyaqian fusedexpressionofsm1chit42proteinsforsynergisticmycoparasiticresponseoftrichodermaafroharzianumonbotrytiscinerea
AT wangxinhua fusedexpressionofsm1chit42proteinsforsynergisticmycoparasiticresponseoftrichodermaafroharzianumonbotrytiscinerea
AT chenjie fusedexpressionofsm1chit42proteinsforsynergisticmycoparasiticresponseoftrichodermaafroharzianumonbotrytiscinerea

Ejemplares similares