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Assessment of Toxoplasma gondii lytic cycle and the impact of a gene deletion using 3D label-free optical diffraction holotomography

Toxoplasma gondii is a widespread single-celled intracellular eukaryotic apicomplexan protozoan parasite primarily associated with mammalian foetal impairment and miscarriage, including in humans. Is estimated that approximately one third of the human population worldwide is infected by this parasit...

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Autores principales: Koutsogiannis, Zisis, Mina, John G. M., Suman, Rakesh, Denny, Paul William
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10433743/
https://www.ncbi.nlm.nih.gov/pubmed/37600951
http://dx.doi.org/10.3389/fcimb.2023.1237594
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author Koutsogiannis, Zisis
Mina, John G. M.
Suman, Rakesh
Denny, Paul William
author_facet Koutsogiannis, Zisis
Mina, John G. M.
Suman, Rakesh
Denny, Paul William
author_sort Koutsogiannis, Zisis
collection PubMed
description Toxoplasma gondii is a widespread single-celled intracellular eukaryotic apicomplexan protozoan parasite primarily associated with mammalian foetal impairment and miscarriage, including in humans. Is estimated that approximately one third of the human population worldwide is infected by this parasite. Here we used cutting-edge, label-free 3D quantitative optical diffraction holotomography to capture and evaluate the Toxoplasma lytic cycle (invasion, proliferation and egress) in real-time based on the refractive index distribution. In addition, we used this technology to analyse an engineered CRISPR-Cas9 Toxoplasma mutant to reveal differences in cellular physical properties when compared to the parental line. Collectively, these data support the use of holotomography as a powerful tool for the study of protozoan parasites and their interactions with their host cells.
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spelling pubmed-104337432023-08-18 Assessment of Toxoplasma gondii lytic cycle and the impact of a gene deletion using 3D label-free optical diffraction holotomography Koutsogiannis, Zisis Mina, John G. M. Suman, Rakesh Denny, Paul William Front Cell Infect Microbiol Cellular and Infection Microbiology Toxoplasma gondii is a widespread single-celled intracellular eukaryotic apicomplexan protozoan parasite primarily associated with mammalian foetal impairment and miscarriage, including in humans. Is estimated that approximately one third of the human population worldwide is infected by this parasite. Here we used cutting-edge, label-free 3D quantitative optical diffraction holotomography to capture and evaluate the Toxoplasma lytic cycle (invasion, proliferation and egress) in real-time based on the refractive index distribution. In addition, we used this technology to analyse an engineered CRISPR-Cas9 Toxoplasma mutant to reveal differences in cellular physical properties when compared to the parental line. Collectively, these data support the use of holotomography as a powerful tool for the study of protozoan parasites and their interactions with their host cells. Frontiers Media S.A. 2023-08-02 /pmc/articles/PMC10433743/ /pubmed/37600951 http://dx.doi.org/10.3389/fcimb.2023.1237594 Text en Copyright © 2023 Koutsogiannis, Mina, Suman and Denny https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Koutsogiannis, Zisis
Mina, John G. M.
Suman, Rakesh
Denny, Paul William
Assessment of Toxoplasma gondii lytic cycle and the impact of a gene deletion using 3D label-free optical diffraction holotomography
title Assessment of Toxoplasma gondii lytic cycle and the impact of a gene deletion using 3D label-free optical diffraction holotomography
title_full Assessment of Toxoplasma gondii lytic cycle and the impact of a gene deletion using 3D label-free optical diffraction holotomography
title_fullStr Assessment of Toxoplasma gondii lytic cycle and the impact of a gene deletion using 3D label-free optical diffraction holotomography
title_full_unstemmed Assessment of Toxoplasma gondii lytic cycle and the impact of a gene deletion using 3D label-free optical diffraction holotomography
title_short Assessment of Toxoplasma gondii lytic cycle and the impact of a gene deletion using 3D label-free optical diffraction holotomography
title_sort assessment of toxoplasma gondii lytic cycle and the impact of a gene deletion using 3d label-free optical diffraction holotomography
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10433743/
https://www.ncbi.nlm.nih.gov/pubmed/37600951
http://dx.doi.org/10.3389/fcimb.2023.1237594
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