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Local phytochrome signalling limits root growth in light by repressing auxin biosynthesis

In nature, plant shoots are exposed to light whereas the roots grow in relative darkness. Surprisingly, many root studies rely on in vitro systems that leave the roots exposed to light whilst ignoring the possible effects of this light on root development. Here, we investigated how direct root illum...

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Autores principales: Spaninks, Kiki, Offringa, Remko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10433924/
https://www.ncbi.nlm.nih.gov/pubmed/37140032
http://dx.doi.org/10.1093/jxb/erad163
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author Spaninks, Kiki
Offringa, Remko
author_facet Spaninks, Kiki
Offringa, Remko
author_sort Spaninks, Kiki
collection PubMed
description In nature, plant shoots are exposed to light whereas the roots grow in relative darkness. Surprisingly, many root studies rely on in vitro systems that leave the roots exposed to light whilst ignoring the possible effects of this light on root development. Here, we investigated how direct root illumination affects root growth and development in Arabidopsis and tomato. Our results show that in light-grown Arabidopsis roots, activation of local phytochrome A and B by far-red or red light inhibits respectively PHYTOCHROME INTERACTING FACTORS 1 or 4, resulting in decreased YUCCA4 and YUCCA6 expression. As a result, auxin levels in the root apex become suboptimal, ultimately resulting in reduced growth of light-grown roots. These findings highlight once more the importance of using in vitro systems where roots are grown in darkness for studies that focus on root system architecture. Moreover, we show that the response and components of this mechanism are conserved in tomato roots, thus indicating its importance for horticulture as well. Our findings open up new research possibilities to investigate the importance of light-induced root growth inhibition for plant development, possibly by exploring putative correlations with responses to other abiotic signals, such as temperature, gravity, touch, or salt stress.
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spelling pubmed-104339242023-08-18 Local phytochrome signalling limits root growth in light by repressing auxin biosynthesis Spaninks, Kiki Offringa, Remko J Exp Bot Research Papers In nature, plant shoots are exposed to light whereas the roots grow in relative darkness. Surprisingly, many root studies rely on in vitro systems that leave the roots exposed to light whilst ignoring the possible effects of this light on root development. Here, we investigated how direct root illumination affects root growth and development in Arabidopsis and tomato. Our results show that in light-grown Arabidopsis roots, activation of local phytochrome A and B by far-red or red light inhibits respectively PHYTOCHROME INTERACTING FACTORS 1 or 4, resulting in decreased YUCCA4 and YUCCA6 expression. As a result, auxin levels in the root apex become suboptimal, ultimately resulting in reduced growth of light-grown roots. These findings highlight once more the importance of using in vitro systems where roots are grown in darkness for studies that focus on root system architecture. Moreover, we show that the response and components of this mechanism are conserved in tomato roots, thus indicating its importance for horticulture as well. Our findings open up new research possibilities to investigate the importance of light-induced root growth inhibition for plant development, possibly by exploring putative correlations with responses to other abiotic signals, such as temperature, gravity, touch, or salt stress. Oxford University Press 2023-05-04 /pmc/articles/PMC10433924/ /pubmed/37140032 http://dx.doi.org/10.1093/jxb/erad163 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Spaninks, Kiki
Offringa, Remko
Local phytochrome signalling limits root growth in light by repressing auxin biosynthesis
title Local phytochrome signalling limits root growth in light by repressing auxin biosynthesis
title_full Local phytochrome signalling limits root growth in light by repressing auxin biosynthesis
title_fullStr Local phytochrome signalling limits root growth in light by repressing auxin biosynthesis
title_full_unstemmed Local phytochrome signalling limits root growth in light by repressing auxin biosynthesis
title_short Local phytochrome signalling limits root growth in light by repressing auxin biosynthesis
title_sort local phytochrome signalling limits root growth in light by repressing auxin biosynthesis
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10433924/
https://www.ncbi.nlm.nih.gov/pubmed/37140032
http://dx.doi.org/10.1093/jxb/erad163
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