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Effect of Stenotrophomonas maltophilia on Tuberculosis

Tuberculosis (TB) is an important infectious disease suffered by many countries, including China. In this stage, accurate diagnosis and treatment are key measures for the prevention and control of TB. Stenotrophomonas maltophilia is a global emerging Gram-negative, multidrug-resistant (MDR) organism...

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Autores principales: Li, Yue, Zhao, Ailan, Yu, Qin, Yu, Nan, Cui, Yao, Ma, Xiaohan, Liu, Haican, Wang, Ruibai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10433947/
https://www.ncbi.nlm.nih.gov/pubmed/37306591
http://dx.doi.org/10.1128/spectrum.00944-23
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author Li, Yue
Zhao, Ailan
Yu, Qin
Yu, Nan
Cui, Yao
Ma, Xiaohan
Liu, Haican
Wang, Ruibai
author_facet Li, Yue
Zhao, Ailan
Yu, Qin
Yu, Nan
Cui, Yao
Ma, Xiaohan
Liu, Haican
Wang, Ruibai
author_sort Li, Yue
collection PubMed
description Tuberculosis (TB) is an important infectious disease suffered by many countries, including China. In this stage, accurate diagnosis and treatment are key measures for the prevention and control of TB. Stenotrophomonas maltophilia is a global emerging Gram-negative, multidrug-resistant (MDR) organism characterized by its high contribution to the increase in crude mortality rates. By single cell preparation and strain identification, we isolated S. maltophilia from stored cultures of Mycobacterium tuberculosis (Mtb). We found that S. maltophilia could not be removed from sputum by alkali treatment or inhibited by antibiotic mixture added to MGIT 960 indicator tubes. When co-cultured with Mtb on a Löwenstein-Jensen (L-J) slant, it could inhibit the growth of Mtb and liquefy the medium. More seriously, it was resistant to 10 of the 12 anti-TB drugs, including isoniazid and rifampin, and made the mixed samples display multidrug-resistant Mtb (MDR-TB) results in the drug sensitivity test, which might change a treatment regimen and increase disease burden. Following, we conducted a small-scale surveillance which showed that the isolation rate of S. maltophilia in TB patients was 6.74%, but these patients had no special characteristics and the presence of S. maltophilia was hidden. The effect of S. maltophilus on TB and its mechanism are unclear and require more attention. IMPORTANCE China is a high-burden country for tuberculosis (TB), multidrug-resistant/rifampicin-resistant tuberculosis (MDR/RR-TB), and HIV-associated TB. Increasing the positive rate of culture and the accuracy of antibiotic susceptibility testing (AST) are important for diagnosis, treatment, and control of TB. In our study, we found that the isolation rate of Stenotrophomonas maltophilia in TB patients was not neglectable and that this bacterium affects the isolation and AST results of TB. Due to a lack of relevant research, the impact of S. maltophilia on the course and outcome of TB is unclear. However, the characteristics of S. maltophilia that increase disease mortality require attention. Therefore, in the clinical testing of TB, in addition to mycobacteria, it is recommended to increase the detection of co-infected bacteria and improve the awareness of TB clinicians of these bacteria.
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spelling pubmed-104339472023-08-18 Effect of Stenotrophomonas maltophilia on Tuberculosis Li, Yue Zhao, Ailan Yu, Qin Yu, Nan Cui, Yao Ma, Xiaohan Liu, Haican Wang, Ruibai Microbiol Spectr Research Article Tuberculosis (TB) is an important infectious disease suffered by many countries, including China. In this stage, accurate diagnosis and treatment are key measures for the prevention and control of TB. Stenotrophomonas maltophilia is a global emerging Gram-negative, multidrug-resistant (MDR) organism characterized by its high contribution to the increase in crude mortality rates. By single cell preparation and strain identification, we isolated S. maltophilia from stored cultures of Mycobacterium tuberculosis (Mtb). We found that S. maltophilia could not be removed from sputum by alkali treatment or inhibited by antibiotic mixture added to MGIT 960 indicator tubes. When co-cultured with Mtb on a Löwenstein-Jensen (L-J) slant, it could inhibit the growth of Mtb and liquefy the medium. More seriously, it was resistant to 10 of the 12 anti-TB drugs, including isoniazid and rifampin, and made the mixed samples display multidrug-resistant Mtb (MDR-TB) results in the drug sensitivity test, which might change a treatment regimen and increase disease burden. Following, we conducted a small-scale surveillance which showed that the isolation rate of S. maltophilia in TB patients was 6.74%, but these patients had no special characteristics and the presence of S. maltophilia was hidden. The effect of S. maltophilus on TB and its mechanism are unclear and require more attention. IMPORTANCE China is a high-burden country for tuberculosis (TB), multidrug-resistant/rifampicin-resistant tuberculosis (MDR/RR-TB), and HIV-associated TB. Increasing the positive rate of culture and the accuracy of antibiotic susceptibility testing (AST) are important for diagnosis, treatment, and control of TB. In our study, we found that the isolation rate of Stenotrophomonas maltophilia in TB patients was not neglectable and that this bacterium affects the isolation and AST results of TB. Due to a lack of relevant research, the impact of S. maltophilia on the course and outcome of TB is unclear. However, the characteristics of S. maltophilia that increase disease mortality require attention. Therefore, in the clinical testing of TB, in addition to mycobacteria, it is recommended to increase the detection of co-infected bacteria and improve the awareness of TB clinicians of these bacteria. American Society for Microbiology 2023-06-12 /pmc/articles/PMC10433947/ /pubmed/37306591 http://dx.doi.org/10.1128/spectrum.00944-23 Text en Copyright © 2023 Li et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Li, Yue
Zhao, Ailan
Yu, Qin
Yu, Nan
Cui, Yao
Ma, Xiaohan
Liu, Haican
Wang, Ruibai
Effect of Stenotrophomonas maltophilia on Tuberculosis
title Effect of Stenotrophomonas maltophilia on Tuberculosis
title_full Effect of Stenotrophomonas maltophilia on Tuberculosis
title_fullStr Effect of Stenotrophomonas maltophilia on Tuberculosis
title_full_unstemmed Effect of Stenotrophomonas maltophilia on Tuberculosis
title_short Effect of Stenotrophomonas maltophilia on Tuberculosis
title_sort effect of stenotrophomonas maltophilia on tuberculosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10433947/
https://www.ncbi.nlm.nih.gov/pubmed/37306591
http://dx.doi.org/10.1128/spectrum.00944-23
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