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A Streptomyces tendae Specialized Metabolite Inhibits Quorum Sensing in Group A Streptococcus

Quorum sensing (QS) is a means of bacterial communication accomplished by microbe-produced signals and sensory systems. QS systems regulate important population-wide behaviors in bacteria, including secondary metabolite production, swarming motility, and bioluminescence. The human pathogen Streptoco...

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Autores principales: Nepomuceno, Vanessa M., Tylor, Kaitlyn M., Carlson, Skylar, Federle, Michael J., Murphy, Brian T., Perez Morales, Tiara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434017/
https://www.ncbi.nlm.nih.gov/pubmed/37284782
http://dx.doi.org/10.1128/spectrum.05279-22
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author Nepomuceno, Vanessa M.
Tylor, Kaitlyn M.
Carlson, Skylar
Federle, Michael J.
Murphy, Brian T.
Perez Morales, Tiara
author_facet Nepomuceno, Vanessa M.
Tylor, Kaitlyn M.
Carlson, Skylar
Federle, Michael J.
Murphy, Brian T.
Perez Morales, Tiara
author_sort Nepomuceno, Vanessa M.
collection PubMed
description Quorum sensing (QS) is a means of bacterial communication accomplished by microbe-produced signals and sensory systems. QS systems regulate important population-wide behaviors in bacteria, including secondary metabolite production, swarming motility, and bioluminescence. The human pathogen Streptococcus pyogenes (group A Streptococcus [GAS]) utilizes Rgg-SHP QS systems to regulate biofilm formation, protease production, and activation of cryptic competence pathways. Given their reliance on small-molecule signals, QS systems are attractive targets for small-molecule modulators that would then affect gene expression. In this study, a high-throughput luciferase assay was employed to screen an Actinobacteria-derived secondary metabolite (SM) fraction library to identify small molecule inhibitors of Rgg regulation. A metabolite produced by Streptomyces tendae D051 was found to be a general inhibitor of GAS Rgg-mediated QS. Herein, we describe the biological activity of this metabolite as a QS inhibitor. IMPORTANCE Streptococcus pyogenes, a human pathogen known for causing infections such as pharyngitis and necrotizing fasciitis, uses quorum sensing (QS) to regulate social responses in its environment. Previous studies have focused on disrupting QS as a means to control specific bacterial signaling outcomes. In this work, we identified and described the activity of a naturally derived S. pyogenes QS inhibitor. This study demonstrates that the inhibitor affects three separate but similar QS signaling pathways.
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spelling pubmed-104340172023-08-18 A Streptomyces tendae Specialized Metabolite Inhibits Quorum Sensing in Group A Streptococcus Nepomuceno, Vanessa M. Tylor, Kaitlyn M. Carlson, Skylar Federle, Michael J. Murphy, Brian T. Perez Morales, Tiara Microbiol Spectr Research Article Quorum sensing (QS) is a means of bacterial communication accomplished by microbe-produced signals and sensory systems. QS systems regulate important population-wide behaviors in bacteria, including secondary metabolite production, swarming motility, and bioluminescence. The human pathogen Streptococcus pyogenes (group A Streptococcus [GAS]) utilizes Rgg-SHP QS systems to regulate biofilm formation, protease production, and activation of cryptic competence pathways. Given their reliance on small-molecule signals, QS systems are attractive targets for small-molecule modulators that would then affect gene expression. In this study, a high-throughput luciferase assay was employed to screen an Actinobacteria-derived secondary metabolite (SM) fraction library to identify small molecule inhibitors of Rgg regulation. A metabolite produced by Streptomyces tendae D051 was found to be a general inhibitor of GAS Rgg-mediated QS. Herein, we describe the biological activity of this metabolite as a QS inhibitor. IMPORTANCE Streptococcus pyogenes, a human pathogen known for causing infections such as pharyngitis and necrotizing fasciitis, uses quorum sensing (QS) to regulate social responses in its environment. Previous studies have focused on disrupting QS as a means to control specific bacterial signaling outcomes. In this work, we identified and described the activity of a naturally derived S. pyogenes QS inhibitor. This study demonstrates that the inhibitor affects three separate but similar QS signaling pathways. American Society for Microbiology 2023-06-07 /pmc/articles/PMC10434017/ /pubmed/37284782 http://dx.doi.org/10.1128/spectrum.05279-22 Text en Copyright © 2023 Nepomuceno et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Nepomuceno, Vanessa M.
Tylor, Kaitlyn M.
Carlson, Skylar
Federle, Michael J.
Murphy, Brian T.
Perez Morales, Tiara
A Streptomyces tendae Specialized Metabolite Inhibits Quorum Sensing in Group A Streptococcus
title A Streptomyces tendae Specialized Metabolite Inhibits Quorum Sensing in Group A Streptococcus
title_full A Streptomyces tendae Specialized Metabolite Inhibits Quorum Sensing in Group A Streptococcus
title_fullStr A Streptomyces tendae Specialized Metabolite Inhibits Quorum Sensing in Group A Streptococcus
title_full_unstemmed A Streptomyces tendae Specialized Metabolite Inhibits Quorum Sensing in Group A Streptococcus
title_short A Streptomyces tendae Specialized Metabolite Inhibits Quorum Sensing in Group A Streptococcus
title_sort streptomyces tendae specialized metabolite inhibits quorum sensing in group a streptococcus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434017/
https://www.ncbi.nlm.nih.gov/pubmed/37284782
http://dx.doi.org/10.1128/spectrum.05279-22
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