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Molecular characterization and genetic diversity of parvoviruses prevalent in cats in Central and Eastern China from 2018 to 2022

Cats are a potential source of genetic diversity for parvoviruses. Herein, 134 samples were collected from cats with clinical gastroenteritis and analyzed for the presence of viral DNA via polymerase chain reaction, which revealed 48 positive samples. Identity analysis of VP2 nucleotide sequences in...

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Autores principales: Pan, Shunshun, Jiao, Ruiqi, Xu, Xin, Ji, Jun, Guo, Ge, Yao, Lunguang, Kan, Yunchao, Xie, Qingmei, Bi, Yingzuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434225/
https://www.ncbi.nlm.nih.gov/pubmed/37601752
http://dx.doi.org/10.3389/fvets.2023.1218810
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author Pan, Shunshun
Jiao, Ruiqi
Xu, Xin
Ji, Jun
Guo, Ge
Yao, Lunguang
Kan, Yunchao
Xie, Qingmei
Bi, Yingzuo
author_facet Pan, Shunshun
Jiao, Ruiqi
Xu, Xin
Ji, Jun
Guo, Ge
Yao, Lunguang
Kan, Yunchao
Xie, Qingmei
Bi, Yingzuo
author_sort Pan, Shunshun
collection PubMed
description Cats are a potential source of genetic diversity for parvoviruses. Herein, 134 samples were collected from cats with clinical gastroenteritis and analyzed for the presence of viral DNA via polymerase chain reaction, which revealed 48 positive samples. Identity analysis of VP2 nucleotide sequences indicated that these 48 strains, belonging to feline panleukopenia virus (FPV) and canine parvovirus type-2 (CPV-2; including new CPV-2a and CPV-2c genotypes), shared 94.59–99.94% nucleotide identity with the reference strains. The FPV strain F8 (isolated from Vietnam) appeared to be a recombinant of strains HB2003 and JS1901, whereas the Chinese CPV-2b strain BM-(11) isolated in 2011 was believed to be a recombinant of strains AH2008 and JS1901. In phylogenetic tree analysis based on VP2 nucleotide sequences, all obtained FPV strains and most reference FPV strains were clustered together, except strain BJ-22, which originated from monkeys. Further, two new CPV-2a strains (AH2005 and AH2008) were close to the newly reported Chinese CPV-2a strains but were distant from the other CPV-2a strains, namely CPV-339 (from the United States) and K022 (from South Korea). Additionally, the FPV and CPV-2 strains had high mutation rates in the antigenic regions of the VP2 protein. According to model prediction of the CPV–VP2 protein, these mutations may cause changes in the tertiary structure of VP2. The findings of this study can be used to improve the pre-evaluation of vaccination efficacy against diseases caused by FPV and CPV-2 in domestic cats and understand their genotypic transmission and mutation trends.
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spelling pubmed-104342252023-08-18 Molecular characterization and genetic diversity of parvoviruses prevalent in cats in Central and Eastern China from 2018 to 2022 Pan, Shunshun Jiao, Ruiqi Xu, Xin Ji, Jun Guo, Ge Yao, Lunguang Kan, Yunchao Xie, Qingmei Bi, Yingzuo Front Vet Sci Veterinary Science Cats are a potential source of genetic diversity for parvoviruses. Herein, 134 samples were collected from cats with clinical gastroenteritis and analyzed for the presence of viral DNA via polymerase chain reaction, which revealed 48 positive samples. Identity analysis of VP2 nucleotide sequences indicated that these 48 strains, belonging to feline panleukopenia virus (FPV) and canine parvovirus type-2 (CPV-2; including new CPV-2a and CPV-2c genotypes), shared 94.59–99.94% nucleotide identity with the reference strains. The FPV strain F8 (isolated from Vietnam) appeared to be a recombinant of strains HB2003 and JS1901, whereas the Chinese CPV-2b strain BM-(11) isolated in 2011 was believed to be a recombinant of strains AH2008 and JS1901. In phylogenetic tree analysis based on VP2 nucleotide sequences, all obtained FPV strains and most reference FPV strains were clustered together, except strain BJ-22, which originated from monkeys. Further, two new CPV-2a strains (AH2005 and AH2008) were close to the newly reported Chinese CPV-2a strains but were distant from the other CPV-2a strains, namely CPV-339 (from the United States) and K022 (from South Korea). Additionally, the FPV and CPV-2 strains had high mutation rates in the antigenic regions of the VP2 protein. According to model prediction of the CPV–VP2 protein, these mutations may cause changes in the tertiary structure of VP2. The findings of this study can be used to improve the pre-evaluation of vaccination efficacy against diseases caused by FPV and CPV-2 in domestic cats and understand their genotypic transmission and mutation trends. Frontiers Media S.A. 2023-07-31 /pmc/articles/PMC10434225/ /pubmed/37601752 http://dx.doi.org/10.3389/fvets.2023.1218810 Text en Copyright © 2023 Pan, Jiao, Xu, Ji, Guo, Yao, Kan, Xie and Bi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Pan, Shunshun
Jiao, Ruiqi
Xu, Xin
Ji, Jun
Guo, Ge
Yao, Lunguang
Kan, Yunchao
Xie, Qingmei
Bi, Yingzuo
Molecular characterization and genetic diversity of parvoviruses prevalent in cats in Central and Eastern China from 2018 to 2022
title Molecular characterization and genetic diversity of parvoviruses prevalent in cats in Central and Eastern China from 2018 to 2022
title_full Molecular characterization and genetic diversity of parvoviruses prevalent in cats in Central and Eastern China from 2018 to 2022
title_fullStr Molecular characterization and genetic diversity of parvoviruses prevalent in cats in Central and Eastern China from 2018 to 2022
title_full_unstemmed Molecular characterization and genetic diversity of parvoviruses prevalent in cats in Central and Eastern China from 2018 to 2022
title_short Molecular characterization and genetic diversity of parvoviruses prevalent in cats in Central and Eastern China from 2018 to 2022
title_sort molecular characterization and genetic diversity of parvoviruses prevalent in cats in central and eastern china from 2018 to 2022
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434225/
https://www.ncbi.nlm.nih.gov/pubmed/37601752
http://dx.doi.org/10.3389/fvets.2023.1218810
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