Cargando…

Insights into the genetic influences of the microbiota on the life span of a host

Escherichia coli (E. coli) mutant strains have been reported to extend the life span of Caenorhabditis elegans (C. elegans). However, the specific mechanisms through which the genes and pathways affect aging are not yet clear. In this study, we fed Drosophila melanogaster (fruit fly) various E. coli...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Fang, Wang, Liying, Jin, Jiayu, Pang, Yulu, Shi, Hao, Fang, Ziyi, Wang, Han, Du, Yujie, Hu, Yufan, Zhang, Yingchun, Ding, Xiaoyue, Zhu, Zuobin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434519/
https://www.ncbi.nlm.nih.gov/pubmed/37601381
http://dx.doi.org/10.3389/fmicb.2023.1138979
_version_ 1785091911023853568
author Zhang, Fang
Wang, Liying
Jin, Jiayu
Pang, Yulu
Shi, Hao
Fang, Ziyi
Wang, Han
Du, Yujie
Hu, Yufan
Zhang, Yingchun
Ding, Xiaoyue
Zhu, Zuobin
author_facet Zhang, Fang
Wang, Liying
Jin, Jiayu
Pang, Yulu
Shi, Hao
Fang, Ziyi
Wang, Han
Du, Yujie
Hu, Yufan
Zhang, Yingchun
Ding, Xiaoyue
Zhu, Zuobin
author_sort Zhang, Fang
collection PubMed
description Escherichia coli (E. coli) mutant strains have been reported to extend the life span of Caenorhabditis elegans (C. elegans). However, the specific mechanisms through which the genes and pathways affect aging are not yet clear. In this study, we fed Drosophila melanogaster (fruit fly) various E. coli single-gene knockout strains to screen mutant strains with an extended lifespan. The results showed that D. melanogaster fed with E. coli purE had the longest mean lifespan, which was verified by C. elegans. We conducted RNA-sequencing and analysis of C. elegans fed with E. coli purE (a single-gene knockout mutant) to further explore the underlying molecular mechanism. We used differential gene expression (DGE) analysis, enrichment analysis, and gene set enrichment analysis (GSEA) to screen vital genes and modules with significant changes in overall expression. Our results suggest that E. coli mutant strains may affect the host lifespan by regulating the protein synthesis rate (cfz-2) and ATP level (catp-4). To conclude, our study could provide new insights into the genetic influences of the microbiota on the life span of a host and a basis for developing anti-aging probiotics and drugs.
format Online
Article
Text
id pubmed-10434519
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-104345192023-08-18 Insights into the genetic influences of the microbiota on the life span of a host Zhang, Fang Wang, Liying Jin, Jiayu Pang, Yulu Shi, Hao Fang, Ziyi Wang, Han Du, Yujie Hu, Yufan Zhang, Yingchun Ding, Xiaoyue Zhu, Zuobin Front Microbiol Microbiology Escherichia coli (E. coli) mutant strains have been reported to extend the life span of Caenorhabditis elegans (C. elegans). However, the specific mechanisms through which the genes and pathways affect aging are not yet clear. In this study, we fed Drosophila melanogaster (fruit fly) various E. coli single-gene knockout strains to screen mutant strains with an extended lifespan. The results showed that D. melanogaster fed with E. coli purE had the longest mean lifespan, which was verified by C. elegans. We conducted RNA-sequencing and analysis of C. elegans fed with E. coli purE (a single-gene knockout mutant) to further explore the underlying molecular mechanism. We used differential gene expression (DGE) analysis, enrichment analysis, and gene set enrichment analysis (GSEA) to screen vital genes and modules with significant changes in overall expression. Our results suggest that E. coli mutant strains may affect the host lifespan by regulating the protein synthesis rate (cfz-2) and ATP level (catp-4). To conclude, our study could provide new insights into the genetic influences of the microbiota on the life span of a host and a basis for developing anti-aging probiotics and drugs. Frontiers Media S.A. 2023-08-03 /pmc/articles/PMC10434519/ /pubmed/37601381 http://dx.doi.org/10.3389/fmicb.2023.1138979 Text en Copyright © 2023 Zhang, Wang, Jin, Pang, Shi, Fang, Wang, Du, Hu, Zhang, Ding and Zhu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhang, Fang
Wang, Liying
Jin, Jiayu
Pang, Yulu
Shi, Hao
Fang, Ziyi
Wang, Han
Du, Yujie
Hu, Yufan
Zhang, Yingchun
Ding, Xiaoyue
Zhu, Zuobin
Insights into the genetic influences of the microbiota on the life span of a host
title Insights into the genetic influences of the microbiota on the life span of a host
title_full Insights into the genetic influences of the microbiota on the life span of a host
title_fullStr Insights into the genetic influences of the microbiota on the life span of a host
title_full_unstemmed Insights into the genetic influences of the microbiota on the life span of a host
title_short Insights into the genetic influences of the microbiota on the life span of a host
title_sort insights into the genetic influences of the microbiota on the life span of a host
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434519/
https://www.ncbi.nlm.nih.gov/pubmed/37601381
http://dx.doi.org/10.3389/fmicb.2023.1138979
work_keys_str_mv AT zhangfang insightsintothegeneticinfluencesofthemicrobiotaonthelifespanofahost
AT wangliying insightsintothegeneticinfluencesofthemicrobiotaonthelifespanofahost
AT jinjiayu insightsintothegeneticinfluencesofthemicrobiotaonthelifespanofahost
AT pangyulu insightsintothegeneticinfluencesofthemicrobiotaonthelifespanofahost
AT shihao insightsintothegeneticinfluencesofthemicrobiotaonthelifespanofahost
AT fangziyi insightsintothegeneticinfluencesofthemicrobiotaonthelifespanofahost
AT wanghan insightsintothegeneticinfluencesofthemicrobiotaonthelifespanofahost
AT duyujie insightsintothegeneticinfluencesofthemicrobiotaonthelifespanofahost
AT huyufan insightsintothegeneticinfluencesofthemicrobiotaonthelifespanofahost
AT zhangyingchun insightsintothegeneticinfluencesofthemicrobiotaonthelifespanofahost
AT dingxiaoyue insightsintothegeneticinfluencesofthemicrobiotaonthelifespanofahost
AT zhuzuobin insightsintothegeneticinfluencesofthemicrobiotaonthelifespanofahost