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Insights into the genetic influences of the microbiota on the life span of a host
Escherichia coli (E. coli) mutant strains have been reported to extend the life span of Caenorhabditis elegans (C. elegans). However, the specific mechanisms through which the genes and pathways affect aging are not yet clear. In this study, we fed Drosophila melanogaster (fruit fly) various E. coli...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434519/ https://www.ncbi.nlm.nih.gov/pubmed/37601381 http://dx.doi.org/10.3389/fmicb.2023.1138979 |
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author | Zhang, Fang Wang, Liying Jin, Jiayu Pang, Yulu Shi, Hao Fang, Ziyi Wang, Han Du, Yujie Hu, Yufan Zhang, Yingchun Ding, Xiaoyue Zhu, Zuobin |
author_facet | Zhang, Fang Wang, Liying Jin, Jiayu Pang, Yulu Shi, Hao Fang, Ziyi Wang, Han Du, Yujie Hu, Yufan Zhang, Yingchun Ding, Xiaoyue Zhu, Zuobin |
author_sort | Zhang, Fang |
collection | PubMed |
description | Escherichia coli (E. coli) mutant strains have been reported to extend the life span of Caenorhabditis elegans (C. elegans). However, the specific mechanisms through which the genes and pathways affect aging are not yet clear. In this study, we fed Drosophila melanogaster (fruit fly) various E. coli single-gene knockout strains to screen mutant strains with an extended lifespan. The results showed that D. melanogaster fed with E. coli purE had the longest mean lifespan, which was verified by C. elegans. We conducted RNA-sequencing and analysis of C. elegans fed with E. coli purE (a single-gene knockout mutant) to further explore the underlying molecular mechanism. We used differential gene expression (DGE) analysis, enrichment analysis, and gene set enrichment analysis (GSEA) to screen vital genes and modules with significant changes in overall expression. Our results suggest that E. coli mutant strains may affect the host lifespan by regulating the protein synthesis rate (cfz-2) and ATP level (catp-4). To conclude, our study could provide new insights into the genetic influences of the microbiota on the life span of a host and a basis for developing anti-aging probiotics and drugs. |
format | Online Article Text |
id | pubmed-10434519 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-104345192023-08-18 Insights into the genetic influences of the microbiota on the life span of a host Zhang, Fang Wang, Liying Jin, Jiayu Pang, Yulu Shi, Hao Fang, Ziyi Wang, Han Du, Yujie Hu, Yufan Zhang, Yingchun Ding, Xiaoyue Zhu, Zuobin Front Microbiol Microbiology Escherichia coli (E. coli) mutant strains have been reported to extend the life span of Caenorhabditis elegans (C. elegans). However, the specific mechanisms through which the genes and pathways affect aging are not yet clear. In this study, we fed Drosophila melanogaster (fruit fly) various E. coli single-gene knockout strains to screen mutant strains with an extended lifespan. The results showed that D. melanogaster fed with E. coli purE had the longest mean lifespan, which was verified by C. elegans. We conducted RNA-sequencing and analysis of C. elegans fed with E. coli purE (a single-gene knockout mutant) to further explore the underlying molecular mechanism. We used differential gene expression (DGE) analysis, enrichment analysis, and gene set enrichment analysis (GSEA) to screen vital genes and modules with significant changes in overall expression. Our results suggest that E. coli mutant strains may affect the host lifespan by regulating the protein synthesis rate (cfz-2) and ATP level (catp-4). To conclude, our study could provide new insights into the genetic influences of the microbiota on the life span of a host and a basis for developing anti-aging probiotics and drugs. Frontiers Media S.A. 2023-08-03 /pmc/articles/PMC10434519/ /pubmed/37601381 http://dx.doi.org/10.3389/fmicb.2023.1138979 Text en Copyright © 2023 Zhang, Wang, Jin, Pang, Shi, Fang, Wang, Du, Hu, Zhang, Ding and Zhu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Zhang, Fang Wang, Liying Jin, Jiayu Pang, Yulu Shi, Hao Fang, Ziyi Wang, Han Du, Yujie Hu, Yufan Zhang, Yingchun Ding, Xiaoyue Zhu, Zuobin Insights into the genetic influences of the microbiota on the life span of a host |
title | Insights into the genetic influences of the microbiota on the life span of a host |
title_full | Insights into the genetic influences of the microbiota on the life span of a host |
title_fullStr | Insights into the genetic influences of the microbiota on the life span of a host |
title_full_unstemmed | Insights into the genetic influences of the microbiota on the life span of a host |
title_short | Insights into the genetic influences of the microbiota on the life span of a host |
title_sort | insights into the genetic influences of the microbiota on the life span of a host |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434519/ https://www.ncbi.nlm.nih.gov/pubmed/37601381 http://dx.doi.org/10.3389/fmicb.2023.1138979 |
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