Cargando…

Exploring retinal ganglion cells encoding to multi-modal stimulation using 3D microelectrodes arrays

Microelectrode arrays (MEA) are extensively utilized in encoding studies of retinal ganglion cells (RGCs) due to their capacity for simultaneous recording of neural activity across multiple channels. However, conventional planar MEAs face limitations in studying RGCs due to poor coupling between ele...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Kui, Liu, Yaoyao, Song, Yilin, Xu, Shihong, Yang, Yan, Jiang, Longhui, Sun, Shutong, Luo, Jinping, Wu, Yirong, Cai, Xinxia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434521/
https://www.ncbi.nlm.nih.gov/pubmed/37600306
http://dx.doi.org/10.3389/fbioe.2023.1245082
_version_ 1785091911587987456
author Zhang, Kui
Liu, Yaoyao
Song, Yilin
Xu, Shihong
Yang, Yan
Jiang, Longhui
Sun, Shutong
Luo, Jinping
Wu, Yirong
Cai, Xinxia
author_facet Zhang, Kui
Liu, Yaoyao
Song, Yilin
Xu, Shihong
Yang, Yan
Jiang, Longhui
Sun, Shutong
Luo, Jinping
Wu, Yirong
Cai, Xinxia
author_sort Zhang, Kui
collection PubMed
description Microelectrode arrays (MEA) are extensively utilized in encoding studies of retinal ganglion cells (RGCs) due to their capacity for simultaneous recording of neural activity across multiple channels. However, conventional planar MEAs face limitations in studying RGCs due to poor coupling between electrodes and RGCs, resulting in low signal-to-noise ratio (SNR) and limited recording sensitivity. To overcome these challenges, we employed photolithography, electroplating, and other processes to fabricate a 3D MEA based on the planar MEA platform. The 3D MEA exhibited several improvements compared to planar MEA, including lower impedance (8.73 ± 1.66 kΩ) and phase delay (−15.11° ± 1.27°), as well as higher charge storage capacity (CSC = 10.16 ± 0.81 mC/cm(2)), cathodic charge storage capacity (CSCc = 7.10 ± 0.55 mC/cm(2)), and SNR (SNR = 8.91 ± 0.57). Leveraging the advanced 3D MEA, we investigated the encoding characteristics of RGCs under multi-modal stimulation. Optical, electrical, and chemical stimulation were applied as sensory inputs, and distinct response patterns and response times of RGCs were detected, as well as variations in rate encoding and temporal encoding. Specifically, electrical stimulation elicited more effective RGC firing, while optical stimulation enhanced RGC synchrony. These findings hold promise for advancing the field of neural encoding.
format Online
Article
Text
id pubmed-10434521
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-104345212023-08-18 Exploring retinal ganglion cells encoding to multi-modal stimulation using 3D microelectrodes arrays Zhang, Kui Liu, Yaoyao Song, Yilin Xu, Shihong Yang, Yan Jiang, Longhui Sun, Shutong Luo, Jinping Wu, Yirong Cai, Xinxia Front Bioeng Biotechnol Bioengineering and Biotechnology Microelectrode arrays (MEA) are extensively utilized in encoding studies of retinal ganglion cells (RGCs) due to their capacity for simultaneous recording of neural activity across multiple channels. However, conventional planar MEAs face limitations in studying RGCs due to poor coupling between electrodes and RGCs, resulting in low signal-to-noise ratio (SNR) and limited recording sensitivity. To overcome these challenges, we employed photolithography, electroplating, and other processes to fabricate a 3D MEA based on the planar MEA platform. The 3D MEA exhibited several improvements compared to planar MEA, including lower impedance (8.73 ± 1.66 kΩ) and phase delay (−15.11° ± 1.27°), as well as higher charge storage capacity (CSC = 10.16 ± 0.81 mC/cm(2)), cathodic charge storage capacity (CSCc = 7.10 ± 0.55 mC/cm(2)), and SNR (SNR = 8.91 ± 0.57). Leveraging the advanced 3D MEA, we investigated the encoding characteristics of RGCs under multi-modal stimulation. Optical, electrical, and chemical stimulation were applied as sensory inputs, and distinct response patterns and response times of RGCs were detected, as well as variations in rate encoding and temporal encoding. Specifically, electrical stimulation elicited more effective RGC firing, while optical stimulation enhanced RGC synchrony. These findings hold promise for advancing the field of neural encoding. Frontiers Media S.A. 2023-08-01 /pmc/articles/PMC10434521/ /pubmed/37600306 http://dx.doi.org/10.3389/fbioe.2023.1245082 Text en Copyright © 2023 Zhang, Liu, Song, Xu, Yang, Jiang, Sun, Luo, Wu and Cai. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Zhang, Kui
Liu, Yaoyao
Song, Yilin
Xu, Shihong
Yang, Yan
Jiang, Longhui
Sun, Shutong
Luo, Jinping
Wu, Yirong
Cai, Xinxia
Exploring retinal ganglion cells encoding to multi-modal stimulation using 3D microelectrodes arrays
title Exploring retinal ganglion cells encoding to multi-modal stimulation using 3D microelectrodes arrays
title_full Exploring retinal ganglion cells encoding to multi-modal stimulation using 3D microelectrodes arrays
title_fullStr Exploring retinal ganglion cells encoding to multi-modal stimulation using 3D microelectrodes arrays
title_full_unstemmed Exploring retinal ganglion cells encoding to multi-modal stimulation using 3D microelectrodes arrays
title_short Exploring retinal ganglion cells encoding to multi-modal stimulation using 3D microelectrodes arrays
title_sort exploring retinal ganglion cells encoding to multi-modal stimulation using 3d microelectrodes arrays
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434521/
https://www.ncbi.nlm.nih.gov/pubmed/37600306
http://dx.doi.org/10.3389/fbioe.2023.1245082
work_keys_str_mv AT zhangkui exploringretinalganglioncellsencodingtomultimodalstimulationusing3dmicroelectrodesarrays
AT liuyaoyao exploringretinalganglioncellsencodingtomultimodalstimulationusing3dmicroelectrodesarrays
AT songyilin exploringretinalganglioncellsencodingtomultimodalstimulationusing3dmicroelectrodesarrays
AT xushihong exploringretinalganglioncellsencodingtomultimodalstimulationusing3dmicroelectrodesarrays
AT yangyan exploringretinalganglioncellsencodingtomultimodalstimulationusing3dmicroelectrodesarrays
AT jianglonghui exploringretinalganglioncellsencodingtomultimodalstimulationusing3dmicroelectrodesarrays
AT sunshutong exploringretinalganglioncellsencodingtomultimodalstimulationusing3dmicroelectrodesarrays
AT luojinping exploringretinalganglioncellsencodingtomultimodalstimulationusing3dmicroelectrodesarrays
AT wuyirong exploringretinalganglioncellsencodingtomultimodalstimulationusing3dmicroelectrodesarrays
AT caixinxia exploringretinalganglioncellsencodingtomultimodalstimulationusing3dmicroelectrodesarrays