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Exploring retinal ganglion cells encoding to multi-modal stimulation using 3D microelectrodes arrays
Microelectrode arrays (MEA) are extensively utilized in encoding studies of retinal ganglion cells (RGCs) due to their capacity for simultaneous recording of neural activity across multiple channels. However, conventional planar MEAs face limitations in studying RGCs due to poor coupling between ele...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434521/ https://www.ncbi.nlm.nih.gov/pubmed/37600306 http://dx.doi.org/10.3389/fbioe.2023.1245082 |
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author | Zhang, Kui Liu, Yaoyao Song, Yilin Xu, Shihong Yang, Yan Jiang, Longhui Sun, Shutong Luo, Jinping Wu, Yirong Cai, Xinxia |
author_facet | Zhang, Kui Liu, Yaoyao Song, Yilin Xu, Shihong Yang, Yan Jiang, Longhui Sun, Shutong Luo, Jinping Wu, Yirong Cai, Xinxia |
author_sort | Zhang, Kui |
collection | PubMed |
description | Microelectrode arrays (MEA) are extensively utilized in encoding studies of retinal ganglion cells (RGCs) due to their capacity for simultaneous recording of neural activity across multiple channels. However, conventional planar MEAs face limitations in studying RGCs due to poor coupling between electrodes and RGCs, resulting in low signal-to-noise ratio (SNR) and limited recording sensitivity. To overcome these challenges, we employed photolithography, electroplating, and other processes to fabricate a 3D MEA based on the planar MEA platform. The 3D MEA exhibited several improvements compared to planar MEA, including lower impedance (8.73 ± 1.66 kΩ) and phase delay (−15.11° ± 1.27°), as well as higher charge storage capacity (CSC = 10.16 ± 0.81 mC/cm(2)), cathodic charge storage capacity (CSCc = 7.10 ± 0.55 mC/cm(2)), and SNR (SNR = 8.91 ± 0.57). Leveraging the advanced 3D MEA, we investigated the encoding characteristics of RGCs under multi-modal stimulation. Optical, electrical, and chemical stimulation were applied as sensory inputs, and distinct response patterns and response times of RGCs were detected, as well as variations in rate encoding and temporal encoding. Specifically, electrical stimulation elicited more effective RGC firing, while optical stimulation enhanced RGC synchrony. These findings hold promise for advancing the field of neural encoding. |
format | Online Article Text |
id | pubmed-10434521 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-104345212023-08-18 Exploring retinal ganglion cells encoding to multi-modal stimulation using 3D microelectrodes arrays Zhang, Kui Liu, Yaoyao Song, Yilin Xu, Shihong Yang, Yan Jiang, Longhui Sun, Shutong Luo, Jinping Wu, Yirong Cai, Xinxia Front Bioeng Biotechnol Bioengineering and Biotechnology Microelectrode arrays (MEA) are extensively utilized in encoding studies of retinal ganglion cells (RGCs) due to their capacity for simultaneous recording of neural activity across multiple channels. However, conventional planar MEAs face limitations in studying RGCs due to poor coupling between electrodes and RGCs, resulting in low signal-to-noise ratio (SNR) and limited recording sensitivity. To overcome these challenges, we employed photolithography, electroplating, and other processes to fabricate a 3D MEA based on the planar MEA platform. The 3D MEA exhibited several improvements compared to planar MEA, including lower impedance (8.73 ± 1.66 kΩ) and phase delay (−15.11° ± 1.27°), as well as higher charge storage capacity (CSC = 10.16 ± 0.81 mC/cm(2)), cathodic charge storage capacity (CSCc = 7.10 ± 0.55 mC/cm(2)), and SNR (SNR = 8.91 ± 0.57). Leveraging the advanced 3D MEA, we investigated the encoding characteristics of RGCs under multi-modal stimulation. Optical, electrical, and chemical stimulation were applied as sensory inputs, and distinct response patterns and response times of RGCs were detected, as well as variations in rate encoding and temporal encoding. Specifically, electrical stimulation elicited more effective RGC firing, while optical stimulation enhanced RGC synchrony. These findings hold promise for advancing the field of neural encoding. Frontiers Media S.A. 2023-08-01 /pmc/articles/PMC10434521/ /pubmed/37600306 http://dx.doi.org/10.3389/fbioe.2023.1245082 Text en Copyright © 2023 Zhang, Liu, Song, Xu, Yang, Jiang, Sun, Luo, Wu and Cai. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Zhang, Kui Liu, Yaoyao Song, Yilin Xu, Shihong Yang, Yan Jiang, Longhui Sun, Shutong Luo, Jinping Wu, Yirong Cai, Xinxia Exploring retinal ganglion cells encoding to multi-modal stimulation using 3D microelectrodes arrays |
title | Exploring retinal ganglion cells encoding to multi-modal stimulation using 3D microelectrodes arrays |
title_full | Exploring retinal ganglion cells encoding to multi-modal stimulation using 3D microelectrodes arrays |
title_fullStr | Exploring retinal ganglion cells encoding to multi-modal stimulation using 3D microelectrodes arrays |
title_full_unstemmed | Exploring retinal ganglion cells encoding to multi-modal stimulation using 3D microelectrodes arrays |
title_short | Exploring retinal ganglion cells encoding to multi-modal stimulation using 3D microelectrodes arrays |
title_sort | exploring retinal ganglion cells encoding to multi-modal stimulation using 3d microelectrodes arrays |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434521/ https://www.ncbi.nlm.nih.gov/pubmed/37600306 http://dx.doi.org/10.3389/fbioe.2023.1245082 |
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