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Long-Distance Electrical and Calcium Signals Evoked by Hydrogen Peroxide in Physcomitrella
Electrical and calcium signals in plants are some of the basic carriers of information that are transmitted over a long distance. Together with reactive oxygen species (ROS) waves, electrical and calcium signals can participate in cell-to-cell signaling, conveying information about different stimuli...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434737/ https://www.ncbi.nlm.nih.gov/pubmed/37233615 http://dx.doi.org/10.1093/pcp/pcad051 |
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author | Koselski, Mateusz Hoernstein, Sebastian N. W Wasko, Piotr Reski, Ralf Trebacz, Kazimierz |
author_facet | Koselski, Mateusz Hoernstein, Sebastian N. W Wasko, Piotr Reski, Ralf Trebacz, Kazimierz |
author_sort | Koselski, Mateusz |
collection | PubMed |
description | Electrical and calcium signals in plants are some of the basic carriers of information that are transmitted over a long distance. Together with reactive oxygen species (ROS) waves, electrical and calcium signals can participate in cell-to-cell signaling, conveying information about different stimuli, e.g. abiotic stress, pathogen infection or mechanical injury. There is no information on the ability of ROS to evoke systemic electrical or calcium signals in the model moss Physcomitrella nor on the relationships between these responses. Here, we show that the external application of hydrogen peroxide (H(2)O(2)) evokes electrical signals in the form of long-distance changes in the membrane potential, which transmit through the plant instantly after stimulation. The responses were calcium-dependent since their generation was inhibited by lanthanum, a calcium channel inhibitor (2 mM), and EDTA, a calcium chelator (0.5 mM). The electrical signals were partially dependent on glutamate receptor (GLR) ion channels since knocking-out the GLR genes only slightly reduced the amplitude of the responses. The basal part of the gametophyte, which is rich in protonema cells, was the most sensitive to H(2)O(2). The measurements carried out on the protonema expressing fluorescent calcium biosensor GCaMP3 proved that calcium signals propagated slowly (>5 µm/s) and showed a decrement. We also demonstrate upregulation of a stress-related gene that appears in a distant section of the moss 8 min after the H(2)O(2) treatment. The results help understand the importance of both types of signals in the transmission of information about the appearance of ROS in the plant cell apoplast. |
format | Online Article Text |
id | pubmed-10434737 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-104347372023-08-18 Long-Distance Electrical and Calcium Signals Evoked by Hydrogen Peroxide in Physcomitrella Koselski, Mateusz Hoernstein, Sebastian N. W Wasko, Piotr Reski, Ralf Trebacz, Kazimierz Plant Cell Physiol Regular Paper Electrical and calcium signals in plants are some of the basic carriers of information that are transmitted over a long distance. Together with reactive oxygen species (ROS) waves, electrical and calcium signals can participate in cell-to-cell signaling, conveying information about different stimuli, e.g. abiotic stress, pathogen infection or mechanical injury. There is no information on the ability of ROS to evoke systemic electrical or calcium signals in the model moss Physcomitrella nor on the relationships between these responses. Here, we show that the external application of hydrogen peroxide (H(2)O(2)) evokes electrical signals in the form of long-distance changes in the membrane potential, which transmit through the plant instantly after stimulation. The responses were calcium-dependent since their generation was inhibited by lanthanum, a calcium channel inhibitor (2 mM), and EDTA, a calcium chelator (0.5 mM). The electrical signals were partially dependent on glutamate receptor (GLR) ion channels since knocking-out the GLR genes only slightly reduced the amplitude of the responses. The basal part of the gametophyte, which is rich in protonema cells, was the most sensitive to H(2)O(2). The measurements carried out on the protonema expressing fluorescent calcium biosensor GCaMP3 proved that calcium signals propagated slowly (>5 µm/s) and showed a decrement. We also demonstrate upregulation of a stress-related gene that appears in a distant section of the moss 8 min after the H(2)O(2) treatment. The results help understand the importance of both types of signals in the transmission of information about the appearance of ROS in the plant cell apoplast. Oxford University Press 2023-05-26 /pmc/articles/PMC10434737/ /pubmed/37233615 http://dx.doi.org/10.1093/pcp/pcad051 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Regular Paper Koselski, Mateusz Hoernstein, Sebastian N. W Wasko, Piotr Reski, Ralf Trebacz, Kazimierz Long-Distance Electrical and Calcium Signals Evoked by Hydrogen Peroxide in Physcomitrella |
title | Long-Distance Electrical and Calcium Signals Evoked by Hydrogen Peroxide in Physcomitrella |
title_full | Long-Distance Electrical and Calcium Signals Evoked by Hydrogen Peroxide in Physcomitrella |
title_fullStr | Long-Distance Electrical and Calcium Signals Evoked by Hydrogen Peroxide in Physcomitrella |
title_full_unstemmed | Long-Distance Electrical and Calcium Signals Evoked by Hydrogen Peroxide in Physcomitrella |
title_short | Long-Distance Electrical and Calcium Signals Evoked by Hydrogen Peroxide in Physcomitrella |
title_sort | long-distance electrical and calcium signals evoked by hydrogen peroxide in physcomitrella |
topic | Regular Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434737/ https://www.ncbi.nlm.nih.gov/pubmed/37233615 http://dx.doi.org/10.1093/pcp/pcad051 |
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