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Integrated transcriptomic and metabolomic profiles reveal the protective mechanism of modified Danggui Buxue decoction on radiation-induced leukopenia in mice

Leukopenia caused by radiation hinders the continuous treatment of cancers. Danggui Buxue Decoction (DBD) has been widely used in clinical owing to low toxicity and definite therapeutic effects to increase leukocytes. Meanwhile, icaritin (ICT) has also been proved to have the effect of boosting peri...

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Autores principales: Chen, Wei, Xin, Jiayun, Wei, Xintong, Ding, Qianqian, Shen, Yunheng, Xu, Xike, Wei, Yanping, Lv, Yanhui, Wang, Jie, Li, Zhanhong, Zhang, Weidong, Zu, Xianpeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434993/
https://www.ncbi.nlm.nih.gov/pubmed/37601071
http://dx.doi.org/10.3389/fphar.2023.1178724
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author Chen, Wei
Xin, Jiayun
Wei, Xintong
Ding, Qianqian
Shen, Yunheng
Xu, Xike
Wei, Yanping
Lv, Yanhui
Wang, Jie
Li, Zhanhong
Zhang, Weidong
Zu, Xianpeng
author_facet Chen, Wei
Xin, Jiayun
Wei, Xintong
Ding, Qianqian
Shen, Yunheng
Xu, Xike
Wei, Yanping
Lv, Yanhui
Wang, Jie
Li, Zhanhong
Zhang, Weidong
Zu, Xianpeng
author_sort Chen, Wei
collection PubMed
description Leukopenia caused by radiation hinders the continuous treatment of cancers. Danggui Buxue Decoction (DBD) has been widely used in clinical owing to low toxicity and definite therapeutic effects to increase leukocytes. Meanwhile, icaritin (ICT) has also been proved to have the effect of boosting peripheral blood cells proliferation. However, there is no study to prove the efficacy of MDBD (Modified Danggui Buxue Decoction), a derivative herbal formula composed of DBD and ICT, in the treatment of radiation-induced leukopenia. In this study, we performed a model of 3.5 Gy whole-body radiation to induce leukopenia in mice. The results of pharmacodynamic studies demonstrated that MDBD could significantly increase the white blood cells in peripheral blood by improving the activity of bone marrow nuclear cells, reducing bone marrow damage, modulating spleen index, and regulating hematopoietic factors to alleviate leukopenia. We also analyzed the integrated results of metabolomics and transcriptomics and found that MDBD could relieve leukopenia and alleviate bone marrow damage by targeting steroid biosynthesis and IL-17 signaling pathway, in which the key genes are Jun, Cxcl2 and Egr1. Therefore, our study provides a basis for the effectiveness and compatibility in the combination of traditional Chinese medicine formula and small molecule drugs.
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spelling pubmed-104349932023-08-18 Integrated transcriptomic and metabolomic profiles reveal the protective mechanism of modified Danggui Buxue decoction on radiation-induced leukopenia in mice Chen, Wei Xin, Jiayun Wei, Xintong Ding, Qianqian Shen, Yunheng Xu, Xike Wei, Yanping Lv, Yanhui Wang, Jie Li, Zhanhong Zhang, Weidong Zu, Xianpeng Front Pharmacol Pharmacology Leukopenia caused by radiation hinders the continuous treatment of cancers. Danggui Buxue Decoction (DBD) has been widely used in clinical owing to low toxicity and definite therapeutic effects to increase leukocytes. Meanwhile, icaritin (ICT) has also been proved to have the effect of boosting peripheral blood cells proliferation. However, there is no study to prove the efficacy of MDBD (Modified Danggui Buxue Decoction), a derivative herbal formula composed of DBD and ICT, in the treatment of radiation-induced leukopenia. In this study, we performed a model of 3.5 Gy whole-body radiation to induce leukopenia in mice. The results of pharmacodynamic studies demonstrated that MDBD could significantly increase the white blood cells in peripheral blood by improving the activity of bone marrow nuclear cells, reducing bone marrow damage, modulating spleen index, and regulating hematopoietic factors to alleviate leukopenia. We also analyzed the integrated results of metabolomics and transcriptomics and found that MDBD could relieve leukopenia and alleviate bone marrow damage by targeting steroid biosynthesis and IL-17 signaling pathway, in which the key genes are Jun, Cxcl2 and Egr1. Therefore, our study provides a basis for the effectiveness and compatibility in the combination of traditional Chinese medicine formula and small molecule drugs. Frontiers Media S.A. 2023-08-03 /pmc/articles/PMC10434993/ /pubmed/37601071 http://dx.doi.org/10.3389/fphar.2023.1178724 Text en Copyright © 2023 Chen, Xin, Wei, Ding, Shen, Xu, Wei, Lv, Wang, Li, Zhang and Zu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Chen, Wei
Xin, Jiayun
Wei, Xintong
Ding, Qianqian
Shen, Yunheng
Xu, Xike
Wei, Yanping
Lv, Yanhui
Wang, Jie
Li, Zhanhong
Zhang, Weidong
Zu, Xianpeng
Integrated transcriptomic and metabolomic profiles reveal the protective mechanism of modified Danggui Buxue decoction on radiation-induced leukopenia in mice
title Integrated transcriptomic and metabolomic profiles reveal the protective mechanism of modified Danggui Buxue decoction on radiation-induced leukopenia in mice
title_full Integrated transcriptomic and metabolomic profiles reveal the protective mechanism of modified Danggui Buxue decoction on radiation-induced leukopenia in mice
title_fullStr Integrated transcriptomic and metabolomic profiles reveal the protective mechanism of modified Danggui Buxue decoction on radiation-induced leukopenia in mice
title_full_unstemmed Integrated transcriptomic and metabolomic profiles reveal the protective mechanism of modified Danggui Buxue decoction on radiation-induced leukopenia in mice
title_short Integrated transcriptomic and metabolomic profiles reveal the protective mechanism of modified Danggui Buxue decoction on radiation-induced leukopenia in mice
title_sort integrated transcriptomic and metabolomic profiles reveal the protective mechanism of modified danggui buxue decoction on radiation-induced leukopenia in mice
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434993/
https://www.ncbi.nlm.nih.gov/pubmed/37601071
http://dx.doi.org/10.3389/fphar.2023.1178724
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