Pre-maturational culture promotes the developmental competence of bovine oocytes derived from an 8-day in vitro growth culture system

In this study, we evaluated the effects of pre-maturational culture (pre-IVM) on the developmental competence of bovine oocytes derived from an 8-day in vitro growth (IVG) culture system. IVG oocytes were subjected to 5 h pre-IVM before in vitro maturation, followed by in vitro fertilization (IVF)....

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Autores principales: CHELENGA, Madalitso, YANAGAWA, Yojiro, KATAGIRI, Seiji, NAGANO, Masashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2023
Materias:
Technology Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10435529/
https://www.ncbi.nlm.nih.gov/pubmed/37197977
http://dx.doi.org/10.1262/jrd.2023-022
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author CHELENGA, Madalitso
YANAGAWA, Yojiro
KATAGIRI, Seiji
NAGANO, Masashi
author_facet CHELENGA, Madalitso
YANAGAWA, Yojiro
KATAGIRI, Seiji
NAGANO, Masashi
author_sort CHELENGA, Madalitso
collection PubMed
description In this study, we evaluated the effects of pre-maturational culture (pre-IVM) on the developmental competence of bovine oocytes derived from an 8-day in vitro growth (IVG) culture system. IVG oocytes were subjected to 5 h pre-IVM before in vitro maturation, followed by in vitro fertilization (IVF). The proportion of oocytes that progressed to the germinal vesicle breakdown stage was similar between groups with and without pre-IVM. Although metaphase II oocytes and cleavage rates after IVF were similar regardless of pre-IVM culture, the blastocyst rate was significantly higher in the group with pre-IVM (22.5%) than without pre-IVM (11.0%, P < 0.05). In conclusion, pre-IVM culture improved the developmental competence of bovine oocytes derived from an 8-day IVG system.
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spelling pubmed-104355292023-08-19 Pre-maturational culture promotes the developmental competence of bovine oocytes derived from an 8-day in vitro growth culture system CHELENGA, Madalitso YANAGAWA, Yojiro KATAGIRI, Seiji NAGANO, Masashi J Reprod Dev Technology Report In this study, we evaluated the effects of pre-maturational culture (pre-IVM) on the developmental competence of bovine oocytes derived from an 8-day in vitro growth (IVG) culture system. IVG oocytes were subjected to 5 h pre-IVM before in vitro maturation, followed by in vitro fertilization (IVF). The proportion of oocytes that progressed to the germinal vesicle breakdown stage was similar between groups with and without pre-IVM. Although metaphase II oocytes and cleavage rates after IVF were similar regardless of pre-IVM culture, the blastocyst rate was significantly higher in the group with pre-IVM (22.5%) than without pre-IVM (11.0%, P < 0.05). In conclusion, pre-IVM culture improved the developmental competence of bovine oocytes derived from an 8-day IVG system. The Society for Reproduction and Development 2023-05-18 2023-08 /pmc/articles/PMC10435529/ /pubmed/37197977 http://dx.doi.org/10.1262/jrd.2023-022 Text en ©2023 Society for Reproduction and Development https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Technology Report
CHELENGA, Madalitso
YANAGAWA, Yojiro
KATAGIRI, Seiji
NAGANO, Masashi
Pre-maturational culture promotes the developmental competence of bovine oocytes derived from an 8-day in vitro growth culture system
title Pre-maturational culture promotes the developmental competence of bovine oocytes derived from an 8-day in vitro growth culture system
title_full Pre-maturational culture promotes the developmental competence of bovine oocytes derived from an 8-day in vitro growth culture system
title_fullStr Pre-maturational culture promotes the developmental competence of bovine oocytes derived from an 8-day in vitro growth culture system
title_full_unstemmed Pre-maturational culture promotes the developmental competence of bovine oocytes derived from an 8-day in vitro growth culture system
title_short Pre-maturational culture promotes the developmental competence of bovine oocytes derived from an 8-day in vitro growth culture system
title_sort pre-maturational culture promotes the developmental competence of bovine oocytes derived from an 8-day in vitro growth culture system
topic Technology Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10435529/
https://www.ncbi.nlm.nih.gov/pubmed/37197977
http://dx.doi.org/10.1262/jrd.2023-022
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