Vidjil add‐on for MRD quantification of samples processed using the EuroClonality‐NGS protocol

Assessment of minimal residual disease in acute lymphoblastic leukemia by immune repertoire NGS requires spiking CDR3 sequences at known quantities into the patient's sample. Recently, the EuroClonality‐NGS group released one of the most comprehensive protocols for this purpose. ARResT/Interrog...

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Detalles Bibliográficos
Autores principales: Giusti, Guilherme Navarro Nilo, Ribeiro, Antonio Vítor, Jotta, Patrícia Yoshioka, Thonier, Florian, Yunes, José Andrés, Meidanis, João
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Short Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10435716/
https://www.ncbi.nlm.nih.gov/pubmed/37601854
http://dx.doi.org/10.1002/jha2.749
Descripción
Sumario:Assessment of minimal residual disease in acute lymphoblastic leukemia by immune repertoire NGS requires spiking CDR3 sequences at known quantities into the patient's sample. Recently, the EuroClonality‐NGS group released one of the most comprehensive protocols for this purpose. ARResT/Interrogate is a closed‐source software for processing these NGS libraries, developed by this same group. Vidjil, an open‐source alternative, currently cannot handle libraries prepared using this protocol. Here, we present a Vidjil add‐on to solve this issue. EuroClonality‐NGS prepared samples analyzed with Vidjil and ARResT/Interrogate were highly concordant (r = 0.998) and presented low error (root‐mean‐square error, RMSE = 0.112).

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